2. Iko kukwirira kwepamusoro kweiyo yako HPLC column
3. Kurongeka kwakanyanya
4. Mashoma mafirita
5. Spell Background ruzha
6.no leachable
Matanho mana eHPLC Sample Kugadzirira
Ngatipeizve kuti iwe unogona sei kuchengetedza nguva nemhinduro dzedu uye panguva imwecheteyo wedzera kubereka kwemhedzisiro yako:
Nhanho 1. Kuongorora kwemvura mhinduro
Hurombo hwemvura mhando ndeimwe yezvinhu zvakakura zvinobatsira kune matambudziko panguva yeHPLC inomhanya. Kusvibiswa kwemvura mufoni mbozha kunogona kudyidzana nekhoramu uye kukonzerwa nekusagadzikana kwekugadzirisa uye kunzwa kusingatarisirwi kana kutarisirwa kutarisirwa muChromatogram, kuwanzotaurwa seghost peaks. Kushandisa mvura yekuwedzera inoderedza njodzi yekutsamwiswa neiyo organic carbons, microorganisms, endotoxins, rnases, uye dnasisi. Mvura yechibereko inowanikwa yekutenga, zvisinei, uchishandisa system yekunatsa yemvura mune yako nzira iri nyore ndiyo nzira yakakwirira yekuve nechokwadi chekupedzisira mvura. Type 1 Reagent mvura yemvura inosangana neASTM, NCCL, ISO, uye USP zviyero zvakanakisa sarudzo yeHPLC application.
Nhanho yechipiri. Kugadzirira kweMitemo
Kugadzirira zviyero zvekuzivikanwa uye huwandu hunogona kuve hwekutya uye hunoparadza nguva. Zvakanyatsoyera solid solix yeiyo soliketi ye solvent inogona kushandiswa yakaoma, uye kutambisa nekuda kwekukanganisa kunogona kuve kunodhura-kunyanya kana uchishanda nemishonga yemishonga. Zvimwe zvinhu zvakadai sekupisa zvakanyanya kugadzirira kugadzirira kugadzirira. Tembiricha yekudziya inoshandura iyo solvent llensity uye inobatsira kusiyana kunoshamisa mukusangana.
Kushandisa chiyero chinogonesa kurongeka kunoitika kunzvimbo dzinoverengeka dzekusvika uye otomatiki maitiro nekubatanidza chiyero kune iyo solvent dispenser inovimbisa zvakanyanya. Uchishandisa software yakadai seiyo yapp16 doosing q-app inogonesa otomatiki kuverenga uye gravimetric yekutarisa tsika dzakayerwa. Kunyora tembiricha uye density yeiyo solvent zvakare inovimbisa kutarisisa kwakakwana, kunyanya kana uchigadzirira zviyero pamazuva akatevera. Direct data kuendesa kubva kuYApp16 Weighing application kune iyo Thermo esainzi ™ 7.2 Chromatography Dha data
Nhanho 3
Kukosha kweruzivo uye chaicho pombi inowanzo kufuratirwa uye kutorwa. Iyo Mushandisi Unyanzvi Level uye ruzivo runogona kuve nehunyanzvi hwakakosha hwekuti unzwe kuongororwa senge HPLC. Kusarudza pipeti kurudyi kunogona kuderedza kukanganisa kwevanhu uye mari yakabatana yemitengo yakatambisa sol.
Mechanika pombi nehoriyamu yekukiya inopa mhinduro iri nyore yekudzivirira mukana wekudzivirira tsaona shanduko, nepo mamwe maitiro emagetsi emagetsi anobvisa mushandisi wekushandiswa kuti aenderere mberi nekuvhurwa. Iyo pipette tipi zvakare yakakosha ruzivo rwekufunga nezveHPLC inoongorora. Yepazasi Yekutungamira matipi akanakisa kana ichishanda pamwe nemari yakasiyana zvevakasiyana, pavanowedzera sampula kupora. Sampula yakasvibiswa inogona zvakare kuitika semhedzisiro yekusvitsa kubva pipette tip. Nekudaro, mazano ane yakakwirira yemakemikari kurwisa kuchengetedza kutendeka kwemuenzaniso.
Nhanho 4. Filter yekunyatsojeka uye Longevity
Kuwedzera kumwe kuriridzwa sampuli isati yanyatso kuongorora kunokonzeresa kuvimbika uye kunogona kuwedzera hupenyu hweHPLC mutsara nekudzivirira macustomer. Syringe mafirita ndiwo mhedziso yakapusa, inokurumidza yekugadzirisa samples dzishoma kuColumn chromatography. Sezvineiwo nePipette matipi, iyo solvent inoshandiswa muHPLC inoda kuti ishandise mafirita ekudzivirira kusimuka uye sampula yakasvibiswa.
Cellulose membranes vanopesana neDimethyl Sulfoxide (DMSO), makiyi, Kotoni, Ester, uye ether makomendi. Zvekunonyanya kuoma sol sol sol sol sol sol sol sol. SAMPLE FILTRATION inova nguva chaiyo-inodya iyo nhamba yemienzaniso inowedzera. Multi-sample filt mariti, senge iyo claristep ® filtration filt system, inogona kuita samples pasina syringes uye nekukurumidza-track-track sampula pasina kubayira pasina kubayira mhando.
Matambudziko mashanu aunofanirwa kutarisisa!
1. Ndeipi hurukuro yakakura kwazvo yakatarisana nematambudziko akatarisana nemaLabs?
Laboratories ari kuwedzeredza kumanikidzwa maererano nenguva uye mutengo, kazhinji kubata kwete chete nehuwandu hwema samples asi zvakare samples dzesangano dzakasiyana siyana. Kugadzirira kweMuenzaniso inzvimbo iyo nzvimbo isinganzwisisike pakati pemhando dzekuenzanisira kana nzira dzekugadzirira nzira uye zvinhu zvinoshandiswa zvinogona kukanganisa mhedzisiro yakawanikwa. Kuverengera kuwirirana pakati pezvishandiso zviri muFiltration mudziyo uye solvent inogona kuderedza nyaya; semuenzaniso, mazinga ezvinyorwa zvinowanikwa izvo zvinogona kukanganisa nekuona kwekuonekwa.
2. Chii chinoitika kana sampuli prep inokanganisa?
Pamberi pekuongorora yakakwira-performance Asingabatsiriro yekuenzanisira inogona kutungamira mukudzikiswa kwehupenyu hwechirume, zvinowedzera zvinodiwa zvehupenyu, zvinogona kuitika zvekushandisa, kuderedzwa mu chromatogration yekubudirira uye kubereka, uye zvimwe zvinetso zvakawanda.
3. Pakutanga kutarisa, filtation inoita kunge iri nyore maitiro ...
Kunyangwe filtration iri inoita kunge chikamu chidiki chebasa rakakura, kukanganiswa kwekusarudza iyo yekurudyi faira yekunyorera inogona kuve yakakura. Semuenzaniso, nzira dzisingabvise chinhu chakaringana chakakosha chinogona kukonzera column kuvharira. Kusarudza zvisina kufanira kwemuenzaniso wekugadzirira kwemaitiro kunogona, pasi pemamwe mamiriro ezvinhu, kusunungura zvinowanikwa mumuenzaniso, kusuma kuongororwa kwekunyepedzera kuburikidza nekutsvuka kurasikirwa - zvese zvinokanganisa kunaka uye kubereka kwemhedzisiro yakaonekwa. Kana iwe uine chivimbo chekuti yako yekupera kwekutadza haisi kupa zvikanganiso izvo zvinoda kuraswa kana kudzima midzi, iwe uchaponesa mari uye uzvipe rugare rwepfungwa.
4. Ndezvipi zvinonyanya kuitika sampuli prep 'inokundikana'?
Chisarudzo chisina kunaka chefaira chesarudzo ndiro dambudziko rimwe ratinoona zvakawanda. Nyaya dzinogona kuoneka seyekunze mapepa kubva kuKutambanudzwa, iyo inogona kukanganisa nekuonekwa kwekuongororwa kwekufarira. Iko kusakwanisa kweiyo firita media ine sampu kana solvent maererano nemakemikari kurwisa hakusi kungogumira chete pakuputsika midhiya. Solvents inogona kukonzera kudhonzwa kushoma kwePolymeric membrane zvinhu kana isingakwanisi kuenderana, iyo inogona kukanganisa maitiro ekushandiswa, zvichitungamira kune zvisina basa zvakachena-kumusoro kwemasampuli. Imwe nzira yekugadzirisa iyi nyaya ndeyekusarudza membrane iyo inoenderana zvakanyanya neese mese uye organic sol sol. Zvekunyatsogadzirira HPLC Kugadzirira, Syringe uye Syringes Mafirita ane Regenerated Cellulose Membranes isarudzo yakanaka nekuda kwekuita kwavo kwakakura solvent.
Iko kushandiswa kwechishandiso chefaira kwakakura pane kudiwa kune vhoriyamu kugadzirira ndeye imwe yakajairika pundutso. Kushandiswa kweimwe-mahombe midziyo yefaira zvinoreva kuti huwandu hwacho hushoma hweMuenzaniso ucharamba uri mufaira, kunyangwe mushure mekutangisa zvinotorwa kuti udzingwe zvakanyanya sezvinobvira.
5. Ndezvipi zvakanakira kutora filtration danho zvakanyanya?
Chinangwa chakakosha chemarabhorari mazhinji akabatanidzwa mukuongororwa kwekuongorora ndeyekudzidzira maitiro anowanzoitika anowanzoitika mhedzisiro - uye izvo zvinoda kutendeuka. Kusarudzwa kweiyo filtration mudziyo nehurongwa hwekuremekedza uye kuchengetwa kwehunyanzvi hunobatsira kuve nekunyatso bvuma kubviswa, nepo sarudzo yehukuru hwakakodzera uye dhizaini yefaira inogona kubatsira kuderedza kurasikirwa kwemuviri. Kufaira kwakanaka kunobatsira zvakare kuchengetedza ikholamu uye zvinogona kutambanudza hupenyu hwehupenyu hwepasi rose nejekiseni.
Mhedziso
HPLC inzira yakakosha yehupenyu hwenyenyenzi uye biopharmaceutical kutsvagirwa sezvo ichinunura mhedzisiro chaiyo, inobereka. Kujeka uku kunoenderana nekuvimbika kweHPLC sampuli, uye nzira yakanakisa yekuve nechokwadi chekutanga-mhando samples kutanga nemidziyo yakanaka. Nemidziyo yeiyo yekuwedzera mvura, kugadzirira kugadzirira, mapipi, matipi, uye mafirita, iyo HPLC sampu yekugadzirira mutsara kune zvese zvakadzama. Otomatiki mhinduro uye software zvakare inobatsira zvakare kuyerera kwechidimbu kugadzirira samples dzakachena dzemhedzisiro dzakachena.
Kukurukurirana kwakawanda nezveHPLC Kuenzanisira Kugadziriswa Solutions inogamuchirwa!
Chirungu
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