I-HPLC yenye yezona ndlela ziqhelekileyo zokuhlalutya. Eyona njongo iphambili: Ukuhambisa iziphumo kunye nezifundo ezithile. Isampulu kufuneka ilungiselelwe ngokufanelekileyo ukuyifaka ngqo kwikholamu ye-HPPC.

Ukuphumeza oku, isampulu yakho ayifanekisi kuphela kwi-solvent efanelekileyo. Eyona nto ibaluleke ngakumbi, kufuneka ingakhululeki kumasuntswana ukuze akwazi ukuphazamisa indlela enokubakho ngexesha lokufumana kunye nokuthintela ubukhulu bekholamu yakho. Le PREAL-IPREURE-SPUNTA SPREAMURE i-PREAMP ihlala ingumsebenzi odangelweyo ochitha ixesha.

Isisombululo se-HPLC Sup

Ukulungiswa kwesampulu kubalulekile kwi-chromatographic kunye ne-spectroscopic hlaluts. Inkqubo yenzelwe ukubonelela ngommeli, isisombululo esifudumeleyo, nesiphithizelayo esilungele inala kwikholamu ye-Chromatographic, kwi-ICP-MS \ / i-cundette okanye i-cubette okanye i-cubette okanye i-cubette okanye i-cubette okanye i-cubette okanye i-cubette okanye i-cubette okanye i-cubette. Injongo yelungiselelo lesampulu kukubonelela nge-aliquot yesampulu ukuba (a) ayinakwenzeka kwikholamu, (b) ayiyi konakalisa ikholamu okanye isixhobo, kwaye (c) ihambelana nendlela ecetywayo.

Xa kuthelekiswa nesampulu ye-prepper kunye nengaphezulu

I-PREAPMP YOKUGQIBELA

I-Chromatogram ngengxolo engasemva kunye ne-peak tailing
Isisombululo se-HPLC Sup

Uhlalutyo lwe-HPLC, iingxaki ezivela kumalungiselelo esampulu zinokwenzeka.

I-1. Ibhloko enokwenzeka yeKholamu yakho ye-HPLC

I-2. Imvakalelo ephantsi yekholamu yakho ye-HPPC

I-3. I-Peak Tailing

4. Iincopho ezinobuxoki ezingathandekiyo

I-5. Ingxolo ephezulu yemvelaphi

I-6. Ngena

Isampulu yesandi esifanelekileyo

I-Chromatogram ene-chromatom ene-chillline yesiseko kunye ne-symetrical peask

Isisombululo se-HPLC Sup

Ukusebenzisa iimveliso zikaSartorius ukulungiselela iisampulu ze-HPPC kuthintela iingxaki eziqhelekileyo ukuba zenzekile kwaye zivumele ukuchaneka kohlalutyo.

I-Blob yekholamu yakho ye-HPLC

2. Imvakalelo ephezulu yekholamu yakho ye-HPPC

3. Ukuchaneka okuphezulu

I-4. Zimbalwa iincopho ezintle

5. Ingxolo engasemva

6.Akwabalekayo

Amanyathelo amane olungiselelo lwesampulu ye-HPLC

Masiqiniseke ukuba ungakongeza njani ixesha kunye nezisombululo zethu kwaye kwangaxeshanye unyusa ukuzala kweziphumo:

Inyathelo loku-1. Uhlalutyo lwezisombululo ezikhulu

Umgangatho wamanzi ahluphekayo yenye yezona zinto zinegalelo elikhulu kwiingxaki ngexesha le-HPPC. Ukungcola kwamanzi kwiSigaba seSoftware kunokunxibelelana nekholamu kwaye kukhokelele kwisisombululo esingalunganga kunye neemvakalelo okanye iincopho ezingalindelekanga kwi-chromatom, ebizwa ngokuba ziincopho zesiporho. Sebenzisa amanzi ophuluko kakhulu kunciphisa kakhulu umngcipheko wokungcolisa iikhabhoni zezinto eziphilayo, i-microorganisms, i-endotoxin, kunye neednases. Amanzi okugqibela ayafumaneka ukuze kuthengwe, nangona kunjalo, esebenzisa inkqubo yokucoca amanzi kwilabhoratri yakho yeyona ndlela ilula yokuqinisekisa amanzi aphezulu ophumeleleyo. Chwetheza iBanga le-1 leBanga lokuHlanganisa elihlanganisana i-ASTM, i-NCCLS, i-ISO, kunye nemigangatho ye-USP yeyona ndlela ilungileyo kwizicelo ze-HPLC.

Inyathelo 2. Ukulungiswa kwemigangatho

Ukulungiselela imigangatho yokwazisa kunye nokuxabana kunokuba yinkqubo edinisayo kunye nexesha lokuchitha. Ngokuchanekileyo ukulinganisa isiseko ukuze ivolumu echazwe kwangaphambili ye-Solvent inokusetyenziswa kunzima, kwaye inkunkuma ngenxa yempazamo inokuxabisa kakhulu-ngakumbi xa isebenza ngamayeza. Ezinye izinto ezinje ngamaqondo obushushu aqhubekeka ngakumbi. Ukuguquguquka kobushushu kuguqula ukungongabi kwesinyibilikihla kwaye ube negalelo kumahluko ophawuleka kakhulu.

Isisombululo se-HPLC Sup

Sebenzisa ibhalansi eyenza ukuba kulunge njengokuthintela ukuchaneka kwiindawo ezininzi zokude kunye nokuzenzela inkqubo ngokudibanisa ibhalansi kwi-dispent yokulahla i-Solvent Shintral ichaza eyona iphambili. Sebenzisa isoftware njenge-YApp16 Doss Q Ukufaka amaqondo obushushu kunye noxinano lwe-solvent iqinisekisa ukugxininisa okuqinisekileyo, ngakumbi xa belungiselela imigangatho kwiintsuku ezilandelayo. Ukudluliselwa kwedatha ngqo kwi-YApp16 Ukulinganisa isicelo kwiNkqubo yeNzululwazi ye-chromeleon ™ 7.2 Inkqubo yeDatha yeDatha (CDS) isoftware yokuphathwa kwedatha engenamaphepha.

Inyathelo 3. Buyela kwiziseko ngombhobho olungileyo

Ukubaluleka komgangatho ochanekileyo kunye nokuchanekileyo kuhlala kujongwa kwaye kuthathwe kancinci. Inqanaba lezakhono zomsebenzisi kunye namava anokuba neempembelelo ezibalulekileyo ekuphonisweni ezibuthathaka njenge-hplc. Ukukhetha umbhobho ofanelekileyo kunokunciphisa impazamo yomntu kunye neendleko ezinxulumene noko zenziwayo.

Isisombululo se-HPLC Sup

Imibhobho yoomatshini eneqhosha levolumu linika isisombululo esilula sokuthintela utshintsho lwe-imeyile, ngelixa imibhobho ye-elektroniki engakumbi ishenxisa umahluko womsebenzisi ngokusebenzisa iivolumu zomsebenzi. Ingcebiso ye-Pipette ineinkcukacha ezibalulekileyo ekufuneka uziqwalasele kuhlalutyo lwe-HLLC. Iingcebiso zokugcina eziphantsi zilunge kakhulu xa zisebenza nezicoci zokuphila ezahlukeneyo, njengoko zikhuthaza ukuphola isampula. Imogaqo-ntle yesampulu inokwenzeka njengokukhokelela kwi-Pipette Tiphu. Kananjalo, iingcebiso ezinesixhathiso esiphezulu sekhemikhali Gcina ingqibelelo yesampulu.

Inyathelo 4. Isihluzo sokuchaneka kunye nobomi

Ukuthatha ukhathalelo olongezelelekileyo kwiisampulu zangaphambi kohlalutyo kuphuhliswa ngokuthembekileyo kwaye kungayindisa ubomi bekholamu ye-HPPC ngokuthintela iziphumo zokuvala. Izihluzo zesirinji sisisombululo esilula, esikhawulezayo sokucoca iisampulu zangaphambi kwekholamu yeKholamu yeCromatography. Njengakwiingcebiso zepatte, izinyibilikisi ezisetyenziswa kwi-HPLC inyanzelisa izihluzo ezithile ukuthintela i-aach kunye nesampulu ungcoliseko.

Isisombululo se-HPLC Sup

I-Selsulose membranes ayinyangeki kwi-DIMETHINE SULFOXID (DMSO), i-Amines, iKeokses, ester, kunye ne-ether. Kukuko ngakumbi izinyibilikisi, i-Polyetheherfone engaqhelekanga (PTFO) i-membrane ithintela ukulelisa kunye nesampulu ungcoliseko. Ukufakela isampulu kuya kuba nexesha lokuthatha ixesha xa inani lesampulu liyanda. Iiyunithi zolimo ezahlukeneyo zesampulu, ezinje ngenkqubo yokuphela i-claristep®, inokucwangcisa iisampulu ngaphandle kwesithole kunye nokulungiswa kwesampulu yesampulu yesampulu yesampulu ngaphandle kokuncama umgangatho.

Iingxaki ezintlanu kufuneka unakekele!

1. Zeziphi ezona ngxaki zikhulu ze-prep ezijongene nee-labs?

Iilebhu ziphantsi koxinzelelo lokwanda ngokwexesha kunye neendleko, zihlala zijongana kuphela ngamanani eesampulu kuphela kodwa neesampulu zamagqabi ohlukeneyo. Ukulungiswa kwesampulu yindawo apho umahluko obufihlakeleyo phakathi kweentlobo zesampulu okanye iindlela zokulungiselela iisampulu kunye nezixhobo ezisetyenzisiweyo zinokuba nefuthe iziphumo ezifunyenweyo. Ukuqinisekisa ukungqinelana phakathi kwezixhobo kwisixhobo esineflling kunye nesinyibilikisi kunokunciphisa imiba; Umzekelo, amanqanaba eendawo ezikhutshiweyo ezinokuthi ziphazamise ihlaziyi.

Isisombululo se-HPLC Sup

2. Kwenzeka ntoni xa i-PAPPPP ihamba iphosakele?

Ngaphambi kokusebenza kwe-chromatography ye-chromatography (i-HLCC), iisampulu zilungiselelwe ngeendlela ezahlukeneyo, zonke ezinokubakho ukuphembelela iziphumo zohlalutyo ngokuchaphazela iSampulukweni. Ukulungiswa kwesampulu engasebenziyo kunokukhokelela ekunciphiseni kobomi, ukunyuka kweemfuno zenkonzo zamacandelo, ixesha elinokubakho lesixhobo, ukunciphisa ukusebenza ngokukuko ngempumelelo kunye nokuzala, nakwezinye iingxaki ezininzi.

3. Ekuqaleni, i-filting ibonakala ngathi yinkqubo elula ...

Nangona ukufihlakele kuyinxalenye ebonakala incinci yokusebenza komsebenzi omkhulu, ifuthe lokukhetha isihluzo sasekunene sesicelo esinikiweyo sinokuba yinto enkulu. Umzekelo, iindlela ezingasusi inxaxheba ngokwaneleyo amathamthane zinokubangela ukuba kuvinjelwe ikholamu. Ukukhetha okungafanelekanga kwezixhobo zesampulu zesampulu, khupha imigaqo-nkqubo ekhutshiweyo kwisampulu, ukuhlalutya inzala okanye ukuzisa iimpazamo kwilahleko yesampulu-yonke imiphumo ebonisiweyo. Ukuba uqinisekile ukuba izidumbu zakho zingafakwanga iimpazamo ezifuna ukusebenzela okanye iingcambu zengca, uya kuyigcina imali, uya kuzinika uxolo lwengqondo.

Isisombululo se-HPLC Sup

4. Yeyiphi eyona meko ixhaphakileyo yesampulu 'iyasilela'?

Ukukhetha okungalunganga kwe-filiter ye-filter ye-filiter yingxaki enye esiyibona kakhulu. Imicimbi inokuvela njengencopho ezingaphezulu kwizinto ezikhutshiweyo, ezinokuphazamisa ukufunyanwa kohlalutyi. Ukungahambelani nemithombo yeendaba yokucoca kunye nesampulu okanye i-solvent ngokuchasene nemichiza ayikabi kuphela kwimpembelelo yokuchitheka kwimidiya yefizitha. Izinyibilikisi zinokubangela ukungcungcutheka okuncinci kwezixhobo ze-polymeric ye-polymeric xa kungahambelani ngokupheleleyo, nto leyo inokuchaphazela ukusebenza okufihlakeleyo, okhokelela ekucoceni ukucoca iisampulu zesampulu. Enye indlela yokujongana nalo mba kukukhetha i-membrane ehambelana ngokubanzi nezinyibilikisi ezinamanzi nezihlathi. I-HLLC yesampulu ye-HPLC, iSiringe kunye neefilitha ezingenasirinji enee-memfines zeselflose ziyindlela elungileyo yendlela elungileyo ngenxa yokuhambelana kwawo okubanzi.

Ukusetyenziswa kwesixhobo sokucoca esikhulu kunokufuneka ivolumu elungiselelwe lolunye umgibe oqhelekileyo. Ukusetyenziswa kwezixhobo zokucoca izihluzo nezincinci kuthetha ukuba isampulu yesampulu iya kuhlala ikwi-filter, nokuba emva kokuthatha amanyathelo okhuseleko kuthathwa ukugxotha kangangoko kunokwenzeka.

Isisombululo se-HPLC Sup

5. Zeziphi izibonelelo zokuthatha inyathelo eliliqinileyo?

Injongo esisiseko yelebhu yelebhu enomdla kuvavanyo lohlalutyo kukuphuhlisa iinkqubo ezixhaphakileyo ezixhasa iziphumo ezingapheliyo- kwaye ifuna ukucoca okungaguqukiyo. Ukukhethwa kwesixhobo esoyikisayo kunye nobukhulu obuchanekileyo besona sixhobo sokugcina ilungelo lokuqinisekisa ukususwa kweempawu ezisebenzayo, ngelixa ukhetho lwesayizi olufanelekileyo kunye noyilo lwecandelo lokucoca lunokunciphisa ukunciphisa ilahleko. Ukuvisa kakuhle kuyanceda ukukhusela ikholamu kwaye kunokubangela ubomi bonke be-ijector kunye nekholamu.

Ukuqukumbela

I-HPLC yindlela ebalulekileyo yobomi kwisayensi yobomi kunye ne-biophamarutical njengoko ihambisa iziphumo ezichanekileyo, ezibuyisiweyo. Oku kuchani kuxhomekeke ekunyanisekeni kwesampulu ye-HPPC, kunye neyona ndlela ilungileyo yokuqinisekisa iisampulu ezikumgangatho ophezulu kukuqalisa ngezixhobo ezilungileyo. Ngezisombululo zamanzi ophulukene, ukulungiselela okusemgangathweni, imibhobho, iingcebiso, kunye neefiltha, i-HPLC ye-akhawunti ye-HPLC ye-akhawunti yazo zonke iinkcukacha. Izisombululo ezenzekelayo kunye nesoftware ikwanceda ukulungisa isampulu yokulungiselela ukuhambisa iisampulu ezicocekileyo kwiziphumo ezicocekileyo.

Iingxoxo ezingaphezulu malunga ne-HLLC SOSMST SOSOSTION ZIYAMKELWA!

Ngaphambili:

Ngaba ukhetha i-cap elungileyo ye-vial yentloko yakho?

Okulandelayo:

Iifayile zeSirinji uya kusetyenziswa?