I-Chromatography ephezulu ye-Iquid Chromatography (HPLC) iyindlela yokuhlaziya ebaluleke kakhulu esetshenziswa emikhakheni ehlukahlukene yesayensi, kusuka kumakhemikhali kanye namakhemikhali ekuqapheni kwezemvelo. Ukunemba nokuthembeka kuncike ezintweni eziningi; Enye into evame ukunganakwa kodwa i-Key Element ikhetha ukufakwa kwama-HPLC amaVials akho; Lokhu kudlala indima ebalulekile ekwakheni kokulungiswa kwesampula nokuhlaziywa, ngakho-ke ukukhetha ukufaka okusebenzayo kubalulekile ukuze uthole i-chromatography ephumelelayo - Lo mbhalo uhlola ukucatshangelwa kokhiye lapho ukhethaIfaka ama-HPLC vials.

Ukuqonda kwemisebenzi '

Ukufakwa yizakhi ezincane ze-cylindrical ezibekwe ngaphakathi kwe-HPLC VIALS ukuzosebenzisa inqwaba yezinjongo ezibalulekile:

1. Ukuqukethwe kwamasampula:Ukufakwa kulungele okuqukethe amasampula akhawulelwe futhi ayigugu, kusiza ukunciphisa ivolumu yazo ngenkathi kusanikeza isivikelo esidingekayo.

2. Yehlisa ivolumu efile:Ukufaka kungasiza ukunciphisa ivolumu efile kuma-vials, okungaphazamisa ukunemba nokuhlaziywa kokulimala.

3. Ukuvimbela ukuhwamuka kwesampula:Ukufakwa kungasiza ekuqinisekiseni ukuhlaziya okunembile ngokukhawulela i-headpace ngaphakathi kwe-vial futhi ngaleyo ndlela kusiza ukuvimbela ukuhwamuka kwesampula. Benza njalo ngokukhawulela isikhala sayo sokuvula ngaphakathi, ngaleyo ndlela bagcina noma yimaphi amaconsi e-liquid anenhloso yokuhlaziywa.

4. Ukuvumelana:Ukufakwa kungakhiwa kusuka ezintweni ezahlukahlukene ukuze kuqinisekiswe ukuhambisana namasampula ahlukile nama-sol.

Ukucatshangelwa okubalulekile okungu-6 lapho ukhetha ukufakwa

Ukukhetha ukufakwa kwe-HPLC kwe-Vial okuhlangabezana nazo zonke izidingo zakho ezithile kubalulekile ukuthola imiphumela enembile nethembekile. Nazi izinto ezimbalwa okufanele uzikhumbule:

1. Isampula yesampula:Ukugcina amasampula ngokufanele, okokuqala thola ivolumu yabo. Ukufakwa kuza ngosayizi abahlukahlukene abamukela amavolumu ahlukahlukene wesampula; Ukukhetha usayizi wokufaka ngokufanele kuzokuvimbela ekuchitheni amasampula ayigugu noma alayishe ngokweqile.

2. Ukuvumelana okubonakalayo:Cabanga ngesampula yakho no-solvent ngaphambi kokukhetha izinto zokufaka ezihambelana. Ukufakwa kungakhiwa ngaphandle kwengilazi, i-polyproplene, noma i-polytetrafluoroethylene (PTFE), phakathi kwabanye; Ukuqinisekisa ukuthi kuzosebenza nakho konke nokuthi noma yikuphi ukuhlangana akubangeli imiphumela engafuneki.

3. Uhlobo lokuhlaziywa:Uhlobo lwakho lokuhlaziywa luneqhaza elinomthelela okufanele lidlalwe lapho ukhetha izinto zokufaka. Isibonelo, ukuhlaziya ukuzwela okuphezulu kungadinga ukungenela ukufakwa kwengilazi ukuze kunciphise ukusebenzisana komhlaba wonke futhi kuqinisekiswe ubumsulwa obuphezulu.

4. Yehlisiwe ivolumu efile:Ukufakwa kuza ngemiklamo ehlukahlukene, njengokufakwa okune-conical neflethi. Ukufakwa kwe-conical kuvame ukunciphisa ivolumu efile ngenkathi kuthuthukisa ukululama kwesampula ngesikhathi umjovo.

5. Ukuvumelana kwesilinganiso:Ngemiphumela efanelekile, qinisekisa ukwakhiwa kwe-Insert ukufana nohlelo lokuvalwa uphawu lwe-vial ukugwema ukuvela noma izindaba zokungcola.

6. Impule Yesampula:Khetha ukufaka okuvikela ubuqotho besampula yakho, ngokwesibonelo uma isihloko saso sokuhwamuka bekungaba ukuhlakanipha ukukhetha eyodwa ngophawu lwezinto ezibonakalayo.

Ukugcina

Ukukhetha iI-HPLC VIAL INSERTLokho kuhlangabezana nazo zonke izidingo zakho zokuhlaziya kubalulekile ekuqinisekiseni ukuhlaziya okunembile nangokwethembekile. Ngokucabanga ngokucophelela izinto ezifana nevolumu yesampula, ukuhambisana kwezinto ezibonakalayo, uhlobo lokuhlaziya, ukuncipha kwevolumu efile kanye nezinkinga zesampula zentengiso ungahle ulungiselele ukulungiswa kwesampula kanye nemijovo ye-HPLC ethembekile. Ungakunaki ukubaluleka kwako; Izakhi ezibonakala zibonakala zincane zinjenge zingenza isitatimende esinomthelela omkhulu ngekhwalithi yedatha ekhiqizwa kusuka ku-HPLC iyahlaziya.

Unentshisekelo ku-HPLC Vial Exents? Ungaphuthelwa le ndatshana efundisayo:I-HPLC Vial Exernts: Ukuthuthukisa Ukunemba Nobuqotho Besampula

Okwedlule:

Umphumela wevolumu ye-vial ekuhlungelweni kwesampula ekuhlaziyweni kwe-chromatography

Olandelayo:

Yini i-cap ye-pre-slit?