Chromatography ndi njira yowunikira yowunikira kwambiri ndi mafakitale ambiri, monga mankhwala ambiri, kusanthula kwachilengedwe ndi chakudya ndi chakumwa. CromaCHography imalola asayansi ndi ofufuza kuti adzipatule, azindikire komanso kuchulukana magawo osiyanasiyana pazinthu zosakanikirana kuti athe kumvetsetsa bwino za zitsanzo za sampu. Mbali imodzi yofunika ya chromatoraphy imaphatikizapo kukonzekera kwamasamu - ntchito yomwe idasewera kudzeraMbale.

Kuzindikira Chromatography ndi Chikumbutso:

Chromachgraphy imagwira ntchito panjira yosinthira mitundu yosiyanasiyana kudzera m'magawo am'manja (monga madzi kapena mpweya) pamisempha yokhazikika (yomwe ingaphatikize zinthu zolimba kapena zamadzi). Izi zimathandiza kuti zikhale zitsanzo zolekanitsidwa kuti zigawo zawo zithe kupyola pamitengo yosiyanasiyana kudzera pamagawo okhazikika ngati ma chromatography (LC), rimbiki iliyonse potengera chikondwerero chawo.

Tanthauzo la kukonzekera kwa zitsanzo:

Asanafufuze pogwiritsa ntchito ma chromatotography, zitsanzo zimayenera kukonzekera zitsanzo zoyenera kuti zitsimikizire zotsatira zabwino komanso zodalirika. Prep imaphatikizapo njira monga kuchotsera, kusefera, kuchepa komanso kudzipatula ngati pakufunika kutengera chikhalidwe chawo.

Njira zokonzekera zachikhalidwe zokonzekera sizingakhale zokwanira poyendetsa votile kapena malo osasunthika; Ndipamene kuwunika kwa mutu kumabwera kumathandiza.

Kusanthula Mutu ndi Mbale Zam'mutu:

Kusanthula kwam'mutu ndi njira ina yowunikitsira mankhwala osasunthika popanda chizolowezi cholowetsa mwachindunji. Zimakhala zothandiza kwambiri pochita ndi mankhwala omwe amawola, kuchitira, kapena kukhala ovuta kusiyanitsa njira wamba.

Mbaleali ndi ziweto zopangidwa mwapadera kuti agwire zitsanzo zonse zamadzimadzi ndi gawo la nthunzi kumwamba. Okonzeka ndi septum ndi kapu yosindikiza molimba motsutsana ndi votiging yolimba motsutsana ndi osasunthika, Mbale Zosasinthika (malo opanda kanthu kuposa madzi) pomwe amadziunjikira. Singano ya syringe ikhoza kugunda septum iyi kuti iduleni zomwe zili mwachindunji mu chromatophy yowunika.

5 Ubwino wa Mbale Mbale Mu chromatography:

Kusunga Chitsanzo:Technology yam'mutu imalepheretsa jakisoni wachifundo chovuta kapena momwe angakhalire ndi zitsanzo za zitsanzo, zomwe zimasunga umphumphu mukamapewa kuipitsidwa.

Chidwi:Chifukwa cha kuchuluka kwawo komwe kumayambitsa mitu yamadzimadzi, mankhwala osasunthika amakonda kuzindikira bwino komwe kumakhalapo m'matauni.

Kulondola Kwakukulu:Kusanthula kwa mutu kumapereka kuchuluka kwa zinthu zosasunthika ndi zovuta zomwe zimagwirizana ndi kupatsirana kosagwirizana ndi matrix a matrix.

Zochepetsedwa matrix zotsatira:Mankhwala osasunthika amatha kuchotsedwa ku matrix awo kuti achepetse zosokoneza ndi zosokoneza matrix mu kusanthula kwa chromatographic.

Ntchito yonse:Mbale zam'madzi zimakhala ndi zovuta zambiri m'mafakitale osiyanasiyana, kuchokera pakuwunikira zinthu zosinthika mu chilengedwe pakuzindikira zotsalira pazakudya ndi zakumwa zina zam'madzi.

Mbalendi chinthu chofunikira kwambiri mu chromatography mu chromatography mu chromatography mu chromatography. Mwa kugwiritsa ntchito Mbale Mpaka Pakafukufuku wawo, ofufuzawo angatsimikize kuti mwakuwona, kuzindikira, ndi kudalirika m'mayeso awo; Kupereka kumvetsetsa kwakukulu mu mtundu wa zitsanzo m'magulu.

4 Mavuto ndi Maganizo a Kusanthula kwa Mutumbe:

Ngakhale kuwunika kwa mutu kumapereka mwayi waukulu, ofufuza ayenera kuthana ndi mavuto ndi malingaliro ena akamawunika:

Kufanana:Kukhazikitsa kufanana pakati pa gawo la nthunzi ndi zitsanzo kumatha kuwononga nthawi ndikuwononga zolondola, zomwe zimathandiza ofufuza kuti adziwe nthawi yayitali ya zitsanzo.

Sampu:Zotsatira za matrix ziyenera kuganiziridwa mukamachita zolondola. Zotsatira za matrix zimatha kukhala ndi vuto la momwe limasinthira masamba osungunulira amasulidwa ndikugawidwa pakati pa madzi ndi nthunzi. Ayenera kupezedwetsa mosamala kuti muchepetse ndikuwonetsetsa kuti ndi chiwerengero chotani.

Kusintha kwa Vial:Mukamasankha mbale yoyenera yowunikira, malingaliro ambiri monga voliyumu, zakuthupi ndi kapu septum uyenera kuzilingalira. Mwachitsanzo, zitsanzo zokhala ndi zipsinjo zapamwamba zitha kukufunirani mavoliyumu akuluakulu kuti mupewe kuthana ndi zitsanzo zawo.

Vesi Vesi Vesi:Kuchuluka kwa zitsanzo zomwe zimayikidwa muzovuta zazovuta za zinthu zomwe zimakhazikika pamtima pamutu pamutu pamutu pawokha, ndikupanga kuchuluka kwa zitsanzo ndi fungulo la kusanthula kwambiri kuti mukwaniritse chidwi chabwino.

Zinthu 6 ndi maluso a kusanthula kwa mutu:

Kusanthula kwam'mutu sikuti kulangidwa; M'malo mwake, chisinthiko chake chimadziwika ndi chidziwitso chopitilira muyeso ndi njira zamakono zamakono zokumana ndi zovuta zingapo:

Mphamvu yamphamvu:Maluso aposachedwa amaphatikizapo kuyeretsa mutu wokhala ndi mpweya wambiri kuti muchotse mankhwala osasunthika, motero nthawi yowonjezera kusanthula mwachangu ndikuwonjezera zitsanzo.

Chokhazikika-chokhazikika cha microxaxtraction (spme):Spme ndi njira yodziwitsa anthu osasunthika molunjika kuchokera pa nsonga pogwiritsa ntchito fiber yokutidwa ndi gawo lokhazikika. Izi zimachotsa kufunika kwa madzi zitsanzo pokonzekera.

Pangani gawo lokongoletsa (SBSE):SBSE imaphatikizapo kugonjetsedwa ndi bala yokhotakhota mu zitsanzo zanu kuti athetse zinthu zosasunthika, kenako ndikuyikanso mu mutu wanu wowunikira - kupereka chidziwitso chachikulu cha matrix zotsatira.

Pakatikati pa Mitundu iwiri yamitundu iwiri (HS-GCXGC):Njira yodziwika bwinoyi imaphatikiza mawonekedwe a masamba awiri okhala ndi nsonga zam'mutu, zomwe zimakulitsa kupatukana bwino kwa zitsanzo zovuta ndi madera ambiri. Njirayi ndiyofunika makamaka mukamachita ndi zitsanzo zokhala ndi zochulukirapo.

Nsonga yokhazikika yokhazikika (HS-SPD):HS-Spde ndi njira yomwe imaphatikiza njira yokhazikika yokhazikika komanso yolimba yam'mutu, ikuwonjezera mphamvu yowonjezera ndi kuchuluka kwa nthawi yowunikira mofulumira ndi nthawi yotembenukira kwambiri.

Maluso Obwezeretsa Mutu:Njira zotere zimaphatikizapo kutchera kusasunthika mankhwala pazinthu zowoneka bwino kenako kuzimangirirani asanasanthule. Kusaka kwa Crortologenic kumathandizanso kumveketsa malire.

Tsegulani chidziwitso ndikuwonjezera chidziwitso chanu cha chromatography.Pezani mayankho omveka bwino kwa mbale 50 hplc faqs mu nkhani iyi: 50 mafunso ambiri omwe amafunsidwa kawiri pa HPLC Mbale

Kugwirizana ndi Kuwongolera Kwabwino:

Kusanthula kwam'mutu kumakhudza gawo lofunikira pakutsimikizira kutsatira malamulo omwe amaperekedwa ndi mafakitale opanga mafakitale. Ndi mabungwe owongolera omwe amakhazikitsa miyezo yapamwamba ya chitetezo chamankhwala ndi mtundu, mawonekedwe owunikira, nsonga yam'mutu imachita gawo lofunikira pakukwaniritsa miyezo imeneyi:

Makampani opanga mankhwala:Kusanthula Kotsalira ndi kiyi yotsatira malangizo a mankhwala. Kusanthula kwa mutu kumalola kuti opanga azindikire mwachangu ndikupanga chilichonse chotsalira chakupanga komwe kwatsala kuti mupange mankhwala omwe sangakhale osatetezeka.

Chitetezo Chakudya ndi Ubwino:Zowonongeka ndi zosintha zachilengedwe monga momwe ma vacs angachititsere zonse zotetezeka komanso zabwino, koma ntchentcheys trends amathandiza kudziwa zodetsa zoterezi kuti zitheke chifukwa cha manja a ogula.

Malangizo Achilengedwe:Kuwunika kwa mpweya ndi madzi ndi mbali zofunika kwambiri kuteteza zachilengedwe, ndi kusanthula kwa mutu kupereka zofunika pazinthu zofunika kuzisankha.

Kugwiritsa ntchito m'makampani osiyanasiyana:

Mbaleapeza kugwiritsa ntchito mafakitale angapo:

Mankhwala:Kusanthula kwamutu ndikofunikira pakuwona ma sodi yotsalira omwe amapezeka mu mankhwala osokoneza bongo ndikuthandizira chitetezo chokwanira ngati mutakhala ngati owopsa amakhala ovomerezeka.

Chakudya ndi Chakumwa:Makina onunkhira amatenga gawo lofunikira popanga zokumana nazo zomwe timasonkhana ndi zakumwa, ndipo chimawunika, ndipo chimakhala cham'mutu chimapereka njira yodziwira ndikupanga zowongolera zazogulitsa komanso zowongolera zoyenera.

Kuwunika zachilengedwe: MbalePerekani njira yabwino yoyesera okhazikika okhazikika (vocs) yomwe ilipo mu mpweya, madzi, ndi zitsanzo za dothi - kuwunika kwangwiro komanso magwiridwe azachilengedwe.

Zokopa: Kusanthula kwam'mutu kumatha kuthandizira ofufuza omwe akuyang'aniridwa pakupezeka kwa zinthu zosasunthika pazinthu zokhudzana ndi zolakwa. Imachita mbali yofunika kwambiri yofufuzira, chizindikiritso chazowonjezera komanso kusanthula mankhwala osokoneza bongo.

Zonunkhira ndi zonunkhira:Kununkhira ndi mafuta ofunikira mafuta nthawi zambiri kumakhala kodabwitsa, nthawi zambiri imakhala ndi masanjidwe mazana ambiri. Kusanthula kwa mutu kumathandizira onunkhira ndipo makola ena amamvetsetsa zovuta zomwe zosakanikirana.

Kupita patsogolo ndi mayendedwe amtsogolo:

Kupita patsogolo kwaukadaulo kwasintha kusintha kwa chromatography, kuphatikiza kuwunika kwa mutu. Kupitiliza Kutha Zinthu Zopangira VIC, Zogwirizanitsa ndi Kusanthula Kwachidziwitso zikuwongolera kulondola ndi kuchita bwino m'matumba; Kuphatikiza apo, kuphatikiza mutu ndi njira zina za chromatographic ngati misa zowoneka bwino.

Khazikitso kafukufuku limafunanso kuthana ndi zovuta monga nthawi yofanana ndi nthawi yofanana, ndikupanga ma protocol osiyanasiyana a mitundu yosiyanasiyana yamitundu yosiyanasiyana imabweretsa zotsatira zodalirika komanso zosasinthasintha.

Kudekha

Mbaleasinthiratu momwe kusatsikiramo zinthu zosasunthika amasanthuliridwa kudzera mu chromatotography. Kufunika kwawo Kusunga Umphumphu ndi Kupititsa patsogolo Kuzindikira sikungakuthandizeni kwambiri, pomwe kugwiritsa ntchito kwawo kumapitilirabe kuthokoza chifukwa cha maphunziro a mafakitale ndi maphunziro asayansi ofanana.

Patsani maluso anu a chromatography pokonzekera maluso lero! Phunzirani maluso agawanika ndi kufooketsa mbale zam'mutu mu nkhani iyi:Zonse za olakwa mphete: kufotokozedwa kwa 13mmm & 20mmm

M'mbuyomu:

Kodi ndingasankhe bwanji septim yoyenera ya HPLC yanga?

Ena:

Mitengo yotsika mtengo ya cromatography ya petrochemistry