Ngaba usebenzisa i-vils ye-HPLC kwilabhoratri yakho?
Ngaba unayo eminye imibuzo malunga ne-vials ye-HPLC kodwa awazi ukuba uqale phi ukufuna iimpendulo?
Kweli nqaku, siqulunqwe ama-50 eyona mibuzo ebuzwa rhoqo malunga I-HPLC Vials Ukukunceda uhambise ilizwe le-chromatography.
ISAHLUKO 1
Zithini i-vials ze-hplc?
Ii-vials ze-HPLC zizinto ezincinci ezisetyenziselwa ukubamba iisampulu ze-romat-right chromatographycy (HLPC).
I-HPLC Vials, okanye i-vial-nty yokusebenza kwe-vials ye-vials, dlala indima ebalulekileyo kuhlalutyo lwe-chemistry kunye nophando lwenzululwazi. Zincinci izikhongozeli ze-cylindrical ezenzelwe ukugcina iisampulu ze-windows zinikwe uhlalutshelo lwetekhnoloji ye-HLPC-indlela eqhubekayo ekwakheni ngokuchanekileyo nangokwahlula-hlula, kunye nokwenza izinto ezingaphakathi kumxube wolwelo.
I-vials esetyenziselwa uhlalutyo ngokwesiqhelo kwivolumu ukusuka kwi-milliliters ezininzi ukuya kwii-milliliters ezininzi kwaye zeza kwiglasi kunye nezinto zeplastiki kunye neplastiki; Yeyiphi oyikhethayo ixhomekeke kwiimfuno zakho ezithile kunye nokuhambelana nesampulu yokuvavanywa.
Yintoni injongo ye-vials ye-HPLC?
I-HPLC Vials zisetyenziselwa ukufumana iisampulu ze-windows zohlalutyo lwe-HPLC, ukuqinisekisa iziphumo ezichanekileyo.
Zenziwe ntoni i-HLPC?
I-vials ye-HPLC yenziwa ngezinto ezahlukeneyo kubandakanya iglasi, i-polypropylene, kunye ne-polythylene.
I-vials ye-hplczidla ngokwenziwa kwiglasi ye-borossilicate, eyaziwa ngokuba yixhathiso eliphezulu le-therm kunye nereyithi ephantsi yokwandiswa. Olu hlobo lweglasi lunokunciphisa umngcipheko woxinzelelo lwe-shirmal ngexesha lokuhlalutya kwe-HLLC. I-vials yeglasi iyabonakala, ivumela uhlolo olubonakalayo lwesampulu kwaye iququzelele ukucoceka okusebenzayo phakathi kokusetyenziswa.
I-vials yeplastiki ye-HPPLzihlala zakhiwa kwizinto ezinjenge-polypropylene, enika ukumelana nemichiza kunye nokuqina. Ezinye ii-vials zeplastiki zenzelwe ukuba ziyi-Intert kwaye zikhululekile kwiizongezo ezinokuthi zikhuphele kwisampula. I-Vials yeplastiki iluncedo xa ujongene neesampulu ezibonakalayo okanye ezisebenzayo, njengoko zinokuxhathisa iichemicals ezithile ezinokunxibelelana neglasi.
Zombini iglasi kunye neplastiki ye-HPLC yeza nezinto ezahlukeneyo zokuvalwa, kubandakanyaI-CAPS CAPS, I-crmimp caps, kwayeI-Snap CAPS, ukubonelela ngokuguquguquka kwiimfuno ezahlukeneyo zelebhu. Ukukhetha kwezinto kunye nokuvalwa kuxhomekeka kwiimfuno ezithile zohlalutyo, ubunjani beesampuli, kunye neenjongo zovavanyo olupheleleyo.
Ndinomdla malunga nezibonelelo zeglasi ye-chromatography yeglasi ngaphezulu kweplastiki? Fumana iimpendulo kweli nqaku lifundisayo:Izizathu ezi-3 eziphezulu zokuba kutheni iGlasi chromatography ye-vials ilunge ngakumbi kune-vials yeplastiki
Zithini iindidi ezahlukeneyo ze-vials ze-HPLC?
Iindidi ezahlukeneyo ze-vials ze-HPLC zibandakanya ukucaciswa, i-amber, i-crimp ephezulu, i-screw ephezulu, iflethi esezantsi, ijikeleze ezantsi, iqumrhu, kwaye ithe ngqo.
Nguwuphi umahluko phakathi kwe-vials ye-hpls ye-hpls?
I-VPLC ye-HPLC ivumela uvavanyo olubonakalayo lwesampulu ngelixa i-amber vials ikhusela iisampulu ezibuthathaka zokukhanya okufihlayo.
Yintoni ubungakanani obuqhelekileyo ye-vial ye-HPPC?
I-vials ye-HPLC iza ngobukhulu obahlukeneyo ukusuka kwi-1.5 ml ukuya kwi-4 ml.
Loluphi olona xinzelelo luphezulu lunokumelana ne-HPPC?
I-vials ye-HPLC inokumelana neengcinezelo ukuya kuthi ga kwi-6000 psi.
Leliphi iqondo lobushushu eliphezulu le-HPPL enokumelana nayo?
I-vials ye-HPLC inokumelana namaqondo obushushu ukuya kwi-121 ° C.
Luthini ubomi beshelf ye-HPLC?
I-vials ye-HPLC inobomi beshelup yeminyaka engama-3-5.
Ndifanele ndigcine njani i-vials ye-HPLC? Amanqaku ayi-10
I-vials ye-HPLC kufuneka igcinwe kwindawo epholileyo, eyomileyo kude nelanga ngqo.
Ukugcinwa ngokuchanekileyo kwe-chromatography ye-chromatography (HPLC) kubalulekile ukukhusela ingqibelelo yesampulu kwaye uphephe ungcoliseko. Nazi ezinye izikhokelo malunga nendlela yokugcina i-vials ye-HPPL:
1.KeNgaphambi kokuba usebenzise, qinisekisa ukuba i-vials kunye ne-CAPS icocekile kwaye ayinamsebenzi. Bahlambe ukusebenzisa izinto ezifanelekileyo zeenyikima kunye neendlela zokuqinisekisa ukucoceka.
2.Utyala le-CAPS efanelekileyo: Khetha i-CAPS efanelekileyo ye-vials yakho - ihlala i-CAPS ye-CAPS okanye i-CRIMP ye-CRPP isetyenziselwa i-vials ye-HPLC-ukubonelela ngokutyhilelwa ngokwaneleyo ngokuchasene ne-evaporation okanye ungcoliseko. Jonga ukuba imeko yabo ihlala ilungile ukuqinisekisa itywina elithintela ukuvuza okanye ungcoliseko.
Ukuphila kakuhle:Emva kokugcwalisa, qinisekisa ukuba i-VPLC yakho ye-HPLC itywinwe ngokuqinileyo ukuthintela ukuphulukana namacandelo okuguqula kunye nongcoliseko. Jonga ukuba nayiphi na iSepta ikhuselekile kwi-caps yabo ngaphandle kweziphene okanye imingxunya ebonakalayo.
Ubushushu obu-4.Xa kuziwa kwi-HPPC yogcino, landela iimeko zokugcina ezicetyiswayo kwisimo sesampulu nganye. Ngokwesiqhelo ezi ziya kuba nobushushu begumbi kodwa abanye banokufuna ifriji okanye ukukhululeka kuxhomekeka kuzinzo lwawo. Landela nayiphi na imeko yokugcina ecetyiswayo esebenza ngokuthe ngqo.
5. Imvakalelo yokukhanya:Ezinye iisampulu zinokuthathela ingqalelo ukukhanya; Kule meko, i-amber okanye izikhongozeli ezifanayo ezibonisa ukukhanya kufuneka zisetyenziselwe ukugcina i-vials yazo ukuze zikhusele ekutyhilekeni ngokuthe ngqo.
6.Lebhu:Ukunceda ukugcina umkhondo kwaye uphephe ukuxuba phakathi kweesampulu, phawula ngokucacileyo i-vial nganye efana negama lesampulu, umhla wokulungiselela, kunye nalo naluphi na ulwazi olongezelelekileyo. Oku kunceda ukugcina yonke into icwangcisiwe.
7.Storage Indawo:Ukunciphisa umngcipheko wokungcola, gcina i-vials yakho ye-HPPC ehlelwe kwindawo ecocekileyo kwaye ilungelelaniswe. Sebenzisa izikhongozeli okanye iiracks zokukhusela ngokuthe tye.
8.Nqanda uzinzo:Gcina iliso kukuzinza kweesampulu zakho. Ezinye iisampulu zinokusona ngokuhamba kwexesha, zenza ukuba ziyimfuneko ukuba zihlalutye ngokukhawuleza ukuze kufezekiswe iziphumo ezichanekileyo.
9.Fifu (kuqala, kuqala):Xa usebenza ngee-vials ezininzi zesampulu enye, zisebenzise ngokulandelelana ngokulandelelana ngokuqalisa i-vial ye-vial kwaye isebenza indlela yakho phambili de kube kuvavanywa zonke iisampulu ngaphambi kokuba zithathelwe ingqalelo okanye zinciphile.
10.Ukuhlolwa:Yenza iitsheki rhoqo kwi-vials yakho egciniweyo ye-HPLC yeempawu zongcoliseko, ukuthunzi okanye ukonakala kwi-caps yazo okanye iSepta. Faka i-vials okanye izinto ezibonisa ukunxiba okanye ukonakala.
Ukugcina okufanelekileyo kunye nokuphatha i-vials ye-HPLC kubalulekile ekufezekisweni kuhlalutyo lwe-chromatographic ngokuchanekileyo, ke nceda ulandele ezi zikhokelo ukukhusela umgangatho kunye nengqibelelo yazo.
ISAHLUKO 2. Ukukhetha i-vials ye-hplc
Zeziphi izinto endifanele ndiziqwalasele xa ndikhetha i-vials ye-hplc?
Xa ukhetha i-vils vials, qwalasela ivolumu yesampulu, ungqinelana nesinyibilikisi, nokuba isampulu kukukhanya.
Ukuqulunqwa kwezixhobo:
Izinto ze-HPPC vial yinto ebalulekileyo. Uninzi lwe-vials ze-HPLC lwenziwe ngeglasi okanye ngeplastiki, nganye inezibonelelo zayo. I-vials yeglasi yi-InErt kwaye ilungele uluhlu olubanzi lweesampulu, ngelixa ii-vials zeplastiki zihlala zikhethwa kuba zikhaphukhaphu kwaye zincinci ekuqaliseni. Ukukhetha phakathi kweglasi kunye neplastiki kuxhomekeke kubunjani besampulu kunye neemfuno zohlalutyo.
Iindidi zeVeni:
Kukho ezahlukeneyoIindidi ze-vials ze-HPPC, kubandakanya i-screw chys vials,I-crimpp ephezulu ye-vials, kwaye i-vials ye-vials. Ukukhetha kuxhomekeke kwisixhobo esithile se-HPPC esisetyenzisiweyo. Umzekelo, ezinye izixhobo zinokufuna uhlobo oluthile lwendlela yokuvala, eya kuba nefuthe kokhetho phakathi kwe-screaw cap kunye ne-crimps ephezulu ye-vials.
Izixhobo zeSeptum:
I-Septum, okanye i-cap liner, licandelo elibalulekileyo elitywina i-vial. Kubalulekile ukuqwalasela umxholo weSeptum, odla ngokusenziwa nge-silicone okanye ptfe. Ukukhetha kuxhomekeke kwizinto ezinje ngokuhambelana nesampula, ukuxhathisa amachiza, kunye neqondo lokuhlaziywa.
Ngaba unomdla wokuqonda i-HPPLC ye-vial ye-vial? Jonga eli nqaku lokuqonda ezibanzi kwimiba ephembelela ekukhetheni uSepta kunye nefuthe lazo kuhlalutyo lweChromatographic:Yintoni i-vial vial sepga?
Ubuninzi beSeptum:
Ubungqingqwa be-Septum bunokuchaphazela amandla okungena kwinaliti kwaye ke ngokunyaniseka kwisampulu. Ukukhetha ubuncwane obufanelekileyo base-Septum kubalulekile ukuqinisekisa itywina elifanelekileyo ngelixa uvumela ukuthotyelwa kwesampulu esebenzayo ngexesha lenaliti.
I-VULUY:
Ithamu le-Vial yimeko ebalulekileyo esekwe kubungakanani besampulu kunye neemfuno ze-HPPC. Ukusetyenziswa kwee-vials ze-vials zesampulu kunciphisa inkunkuma yesampulu kwaye kuqinisekiswe iziphumo ezichanekileyo nezibuyisiweyo.
Ndizi njani ukuba loluphi uhlobo lwe-vial ye-HPPL ukuba uyisebenzise isicelo sam?
Khetha uhlobo lwe-vial ye-HPPC esekwe kwivolumu yesampulu, ihambelana nesinyibilikisi, kwaye nokuba isampulu kukukhanya.
Nguwuphi umahluko phakathi kwe-screw caps kunye ne-crmimp cap vials vials?
I-vials ekhutshiweyo ye-HPLC Funa isixhobo sokuqhekeza ukutywina i-vial ngelixa i-screw-cap ye-vials inokuthi itywinwe ngesandla.
Nguwuphi umahluko phakathi kweflethi kunye ne-vials ejikeleziweyo ye-HPLC?
Iflethi-ezantsi i-vials ye-HIPLC iZinzile ngakumbi ngelixa i-HPLC ye-HPLC ilula ukuyiphatha.
Iflethi-ezantsi i-vials vialszidume ngokuqina kwazo. Iindlela zabo zeflethi zabo ziqinisekisa ukuba bahlala ngokuqinileyo kuwo nawuphi na umphezulu othe tyaba, ubenza ukuba babe nokhetho oluhle lweenkqubo zokuphatha ngesampulu, isampulu yerobhothi, iitreyitha zokugcina, kunye neetreyitha zesampulu. Indawo yabo ekhuselekileyo kodwa yendawo yokuhlala inciphisa umngcipheko wengcebiso ye-vial-ngaphezulu-into ebaluleke ngakumbi kwiilebhu eziphezulu zokwenza izinto ezifuna ukuchaneka.
Ngokwahlukileyo, umjikelo we-HPLC vils uyathandwa ngenxa yokuphatha kwabo. Iziseko zazo ezijikelezileyo zenza ukuba kubekwe indawo egudileyo kwii-vial racks okanye izixhobo kunye nokulayisha ngokukhululekileyo kunye nokulayisha; Ukwenza ukuba ezi vils zilungele izicelo zokuphatha ngesandla zesandla apho ukusetyenziswa ngokulula kunye nokulula lula kubaluleke kakhulu.
Nguwuphi umahluko phakathi kwe-vials ye-HPPC?
I-Coyinical Vials Yiba nobume obuqhelekileyo ngelixa ngqo i-vials ye-HPPC inemilo ethe tye.
Nguwuphi umahluko phakathi kwento esebenzayo kwaye engasebenziyo ye-HPLC?
I-vials ehleliweyo ye-HPLC ine-caials ekhethekileyo yokunciphisa isampulu ngelixa i-vials engasebenziyo ye-HPPL ayisebenzi.
Nguwuphi umahluko phakathi kwe-pre-slit kunye ne-plit ye-plit ye-septa?
I-Pre-Slit Septa Yiba ne-slit embindini ukuze ungenise inaliti elula ngelixa uSept-Slit Septa ufuna inaliti yokuphosa iSepta.
Isikhokelo sobuchwephesha ekukhetheni phakathi kwesilayidi esingaphambi kwesilayidi okanye esingayityisiyo septa, sukuphoswa kweli nqaku:Ungayikhetha njani i-Septa Pal-Slit okanye hayi?
Ithini indima ye-septa kwi-vials ye-HPLC?
SeptaInika umqobo phakathi kwesampulu kunye nendawo esingqongileyo, ukuthintela ungcoliseko kunye nokuphuma.
Ngaba zikho ezinye iinkalo ezithile zokukhetha i-vials ye-HPPC yesampulu eguqukayo?
Khetha i-vils ye-HPPL ene-septum ephahlayo ye-septum yeesampulu eziguqukayo.
Ngaba zikho naziphi na iimbono ezikhethekileyo ngokukhetha i-vials ye-biolsc kwizicelo ze-byphapharmacemaster?
Khetha i-vials ye-HPLC enendawo ye-adsorction ye-Biophapharmication.
ISAHLUKO 3. Sebenzisa i-HPLC Vials
Ndiyilungiselela njani i-vial ye-HPPC ukuze isetyenziswe?
Coca i-vial enesiseko esifanelekileyo, faka i-septum, kwaye ugcwalise i-vial ngesampula.
Ukuba unomdla wokungafikeleli kwi-HPLC yesampulu ye-HPLC, ungaphoswa ukuqonda okunikezwe kweli nqaku:I-HLLC yesampulu yokulungiselela ukulungiselela iziphumo ezilungileyo
Ndiyikhetha njani inaliti efanelekileyo ye-HPPC yam?
Khetha inaliti ene-gauge kunye nobude obude bomthamo kunye novimba.
Ndikhetha njani isirinji elungileyo kwi-vial yam ye-hplc?
Khetha isirinji ngevolumu efanelekileyo kunye nokuhambelana nesinyibilikisi.
Ndifaka njani i-septum kwi-vial ye-HPPL?
Sebenzisa isixhobo esifakelweyo se-septum ukufaka i-septum kwi-vial vial vial.
Ndiyisusa njani i-septum kwi-vial vial vial?
Sebenzisa isixhobo sokususa iSeptum ukususa i-septum kwi-vial vial vial.
Ndicoca njani i-hplc vial?
Coca i-vial ye-HPPC Ngokuthoba nge-solvent efanelekileyo kwaye ivumele yomile ngokupheleleyo.
Ukufumana ulwazi olupheleleyo malunga nokucoca isampulu ye-chiatography ye-vials, ungaphoswa linqaku eliqinileyo:Iyasebenza kakuhle! Iindlela ezi-5 zokucoca iimodeli ze-chromatographys
Ngaba i-HLPC i-vials ingasetyenziswa?
Akucetyiswa ukuba uqhubeke kwakhona i-vials vials njengoko inokukhokelela kwiziphumo ezichanekileyo ngenxa yongcoliseko.Ngelixa kunokubonakala ngathi yinto ekhuselekileyo yeendleko, kukho izizathu ezininzi ezibalulekileyo zokuphepha ukuphinda ziphinde ziphinde ziphinde ziphinde ziphinde ziphindezezi:
Umngcipheko wongcoliseko:I-vialc vials kufuneka icoceke kwaye ingenazintsalela okanye iintsalela. Nditsho nezixa zereyiti zesampulu ezahlalutyo ngaphambili zingakhokelela ekungcoliseni ukungcoliseka, kuchaphazele ukuchaneka kweziphumo zakho.
Impendulo yesampulu:Ukuqinisekisa ukuthembeka kweesampulu zakho, kubalulekile ukusebenzisa i-vials ecocekileyo, engacocekanga yohlalutyo ngalunye. Ukuqesha i-Vials kunokwazisa ukungafezeki okanye izinto zangaphandle, okunokwenzeka ekusebenziseni ukwakhiwa kweesampulu zakho.
Ukuphulukana nokuchaneka:I-HPLC yindlela yokuhlalutywa kwe-HPLC. Ukuqesha i-vills kunokukhokelela kumahluko okwimo yesampulu, utshintsho kwi-PH, okanye ukungeniswa kokungcola, konke okunokukhokelela ekunciphiseni ngokuchanekileyo nangokuzala.
Ixesha kunye nokusebenza kwexabiso:Ngelixa ukuqesha ukuqesha i-vials kunokubonakala kusebenza ngendleko, ixesha kunye nomzamo ofunekayo wokucoca ngokucokisekileyo kunye nokuqinisekiswa kococeko lwe-Vial kunokwenza ukuba loo nto ingagciniweyo.
Ulawulo loMgangatho kunye nokuthotyelwa komthetho:Iilebhu ezininzi zilandela ngqo kulawulo olungqongqo ngokungqongqo kunye nemigangatho yolawulo engathintela ukusetyenziswa kwakhona kwee-vills ngenxa yemingcipheko enxulumene noko. Ukuthobela le migangatho kubalulekile ekuthembekeni nasekuthembekeni kophando lwakho.
Mangaphi amaxesha endinokusebenzisa i-vial vial vial?
Sebenzisa i-vial entsha ye-HPPL kuhlalutyo ngalunye ukunqanda ungcoliseko kunye nokuqinisekisa iziphumo ezichanekileyo.
Ndilahla njani i-vials esetyenziswe njani?
Lahla i-vials esetyenzisiweyo ye-HPLC ngokwemigaqo yendawo yenkunkuma eyingozi.
Ndiluthintela njani ungcoliseko lwe-vials yam ye-HPPL?
Ukunciphisa ukubonakaliswa kwe-HPPC ye-HPPC kwindalo kwaye uphatha i-vial ngezandla ezicocekileyo.
Kutheni le nto sisebenzisa i-vial yeplastiki yokuchongwa kwe-ceracy kwi-hplc?
I-vials zeplastiki zihlala zisetyenziselwa ukufunyanwa kwe-ceration kwi-chromatography ye-chromatography (i-HPPC) ngenxa yezizathu ezininzi:
Ukuhambelana kwamachili: I-vials zeplastiki zihlala zenziwa zizixhobo ezinjenge-polyproplene okanye i-polybethylene, i-Inert ye-Inert kwaye ihambelane noluhlu olubanzi lwee-slinyis kunye nematriki esetyenziswe kuhlalutyo lwe-HPPC. Oku kuqinisekisa ukuba izinto ze-Vial aziphazamisi uhlalutyo okanye ziphendule kwiinkampu ezahlukeneyo.
I-adsorption esezantsi: I-Vials yePlasikhi yePlasiine ineempawu ezisezantsi ze-adsorption xa kuthelekiswa nee-vials zeglasi. I-Adsorvotion ingakhokelela ekubeni ilahleko yesampulu okanye ukutshintsha kohlalutyo, ngakumbi ekuhlalutyeni ngohlalutyo okanye xa ujongene nohlalutyo olunobuthathaka. Ngokusebenzisa i-vials yeplastiki, umngcipheko we-adsorption incitshisiwe, okukhokelela kwiziphumo ezichanekileyo nezichanekileyo.
Ukuncitshiswa komngcipheko wesaphulelo: I-vials zeplastiki azinantsingiselo yokwahlukana xa kuthelekiswa ne-vials yeglasi. Oku kuyingenelo ngakumbi kwiilebhu eziphezulu zokufaka apho iinkqubo zesampula zibandakanya ukuphathwa ngokuphathwa rhoqo kwe-vial kunye nezothutho. Ukuqina kwe-vials zeplastiki kunciphisa amathuba okulahleka kwesampulu ngenxa yokwaphuka, ngaloo ndlela kuphucule ukusebenza kakuhle kunye nokunciphisa iindleko.
Ukusebenza kweendleko: I-Vials zeplastiki zihlala zixabisa kakhulu kune-vials yeglasi, ibenza babe ngabakhethiweyo kwii-vials ze-HPPC ze-HPPC apho kusetyenziswa khona amanani amakhulu eevisha. Ukuqwalaselwa kweendleko kubaluleke kakhulu kwiilebhu ezisebenza phantsi kwezithintelo zohlahlo-lwabiwo mali okanye ukwenza uhlalutyo oluqhelekileyo.
Ukupheliswa kokujongana nokugcinwa: I-vials zeplastiki zilula kwaye zilula ukusingatha, zibenza zibe lula kubasebenzi belebhu ngexesha ukulungiselela isampulu, inaliti, kunye nokugcinwa. Ukongeza, i-vials yeplastiki ihlala ifumaneka kwiifomathi ezahlukeneyo, kubandakanya i-snap-cap okanye i-screw-cap yengqokelela, enika ukuguquguquka kwiindlela zokukhetha kunye nokusetyenziswa ngokulula.
Ngokubanzi, ukusetyenziswa kwee-vials zeplastiki zokuchongwa kwe-CLPC kubonelela ngeendlela ezininzi, kubandakanya nokuhambelana nemichiza, ukuncitshiswa komngcipheko, ukuncitshiswa kwexabiso, kunye nokugcina iilebhu ezininzi ezithandwayo.
ISAHLUKO 4
Kutheni le nto i-vials yam ye-hplc ivuza? Izinto ezisi-7
Qinisekisa ukuba i-septum ifakwe ngokufanelekileyo kwaye i-vial ayigcwaliswanga.
1. Itywina le-cap:Esona sizathu siqhelekileyo sokuvuza i-vial yitywina elingalunganga phakathi kwe-vial kunye ne-cap. Oku kunokwenzeka ngenxa yezizathu ezininzi:
1.1 Ukuqinisa ngaphezulu:Ukuba uqinisekisa i-cap kakhulu, inokuqhekeka kwe-septum, okobangela ukuvuza. Sebenzisa i-torque ecetyiswayo okanye ekrwada kwindibaniselwano yakho ye-vial kunye ne-cap.
1.2 Ukuqina okungaphantsi kwe-1.2Ukuba i-cap ayiqinisekanga ngokukhuselekileyo, isenokunganikezeli itywina elaneleyo, ivumela ukuvuza ukuba kwenzeke. Qinisekisa ukuba ufaka uxinzelelo olufanelekileyo xa ukutywina i-vial.
1.3 I-CAPS eyonakeleyo:Jonga nawuphi na umonakalo obonakalayo okanye isiphene kwiCAPS, ezinje ngee-rocks okanye izitenxo, ezinokuthi zithintele itywina elifanelekileyo.
I-2.SETMPM esemgangathweni:I-Septum, inxalenye yerabha okanye i-silicone inxalenye ye-cap, idlala indima ebalulekileyo ekuthinteleni ukuvuza. Imiba enoSeptum inokukhokelela ekuvuzaneni, njenge:
2.1 Ukungahambi kakuhle:Ukuba i-septum ihlatywe amaxesha amaninzi, inokuhlakulela imingxunya kwaye iphulukane nokukwazi ukutywina ngokufanelekileyo.
2.2 nxiba kunye nokukrakra:Ixesha elingaphezulu, iSepta inokude inciphise okanye inciphise i-elastic, inciphisa ukusebenza kwawo. Guqula i-septa rhoqo okanye njengoko kucetyiswa ngumenzi.
I-3. Umonakalo we-vial:Umonakalo kwi-vil ngokwayo, njengokuqhekeka okanye iitshipsi, kungalalanisa ukuthembeka kwetywina, okukhokelela ekuvuzaneni. Vavanya i-vials yeziphene ezibonakalayo kwaye zithathe indawo eyonakalisiweyo.
4. Ungcoliseko:Ungcoliseko kwi-Vial okanye kwindawo ye-cap inokuphazamisa itywina. Qinisekisa ukuba zombini ii-vials kunye nee-CAPS zicocekile kwaye zikhululekile kuzo naziphi na iintsalela okanye ingcoliseko.
I-5. Isampulu yoxinzelelo:Ezinye iisampulu zinokwenza uxinzelelo ngaphakathi kwe-vial ngenxa yobukho beegesi okanye izinto eziguquguqukayo. Ukuba i-cap ayitywinwa ngokuqinileyo okanye ukuba i-septum ayihambelani nomgangatho ophezulu, olu uxinzelelo lunokukhokelela ekuvuzaneni.
6. TUnxibelelwano lwe-IDUtshintsho lobuchule obunobunkumelo lunokubangela umoya ngaphakathi kwe-vial yokwandisa okanye ikhontrakthi, inokubandakanya itywina. Vumela i-vials ukulinganisa ubushushu belebhu ngaphambi kohlalutyo.
I-7. Vial kunye ne-capQinisekisa ukuba i-vial nentloko oyisebenzisayo iyahambelana. Sebenzisa izinto ezivela kubavelisi abahlukeneyo kunokubangela ukuba amatywina ahluphekayo.
Ndikuthintela njani i-vials yam ye-hplc yokuqhekeza?
Phatha i-vial vial vial ngononophelo kwaye uphephe ukulahla okanye ukuyiphosa.
Ndikuthintela njani i-vials yam ye-hplc evela ekuqhekekeni?
Gcina i-vils ye-HPPC kwisikhongozeli efanelekileyo kwaye kuthintele ukubonakaliswa kwamaqondo obushushu agqithisileyo.
Ndikuthintela njani i-vials yam ye-HPLC ukuba ingabinamathe kunye?
Gcina i-vils ye-HPPC kwindawo eyomileyo kude nomswakama.
Kutheni le nto i-vials yam ye-HPPC ibambelele kwi-autosamampler?
Qinisekisa i I-HPPC vial iyahambelana ne-autosampler kwaye i-vial ihleli ngokufanelekileyo.
Ndiyisusa njani i-vial ye-hplc ye-hplc evela kwi-autosampler?
Sebenzisa isixhobo sokususa i-vial ukususa i-vial ye-HPPC evela kwi-autosampler.
Ndithintela njani i-vials yam ye-HPLC evela ekufumaneni umnqamlezo?
Sebenzisa i-vial entsha ye-HPLC kwisampulu nganye kwaye uphephe ukuba ne-septum.
Ndithintela njani i-vials yam ye-hplc evela ekuphathweni konakaliswe ngexesha lothutho?
Sebenzisa isitya esifanelekileyo sezothutho kunye nokujongana nononophelo.
Ndikuthintela njani i-vials yam ye-HLPC ekubeni iphulukane netywina labo?
Qinisekisa ukuba i-septum ifakwe ngokufanelekileyo kwaye i-vial ayigcwaliswanga.
Kutheni i-vials yam ye-hplc ivaliwe?
I-vials evaliweyo ye-HPLC inokubangelwa kukungcolisa isampula okanye ungcoliseko.
Icandelo 5. Ukulawulwa komgangatho we-HPPC
Ubungakanani be-HPLC ye-HPLC?
I-HLLC esemgangathweni ye-HLLC (i-deightie-i-chromatography) i-vials ihlala iza ngobukhulu obubini obuqhelekileyo,I-2 ml kunye ne-1.5 ml. Ngelixa ezi zixhobo zisetyenziswa kakhulu kwizicelo ze-HLLC, obunye ubukhulu obufana ne-4 ml kunye ne-0.5 ml nayo inokulunga kuxhomekeka kwivolumu yesampulu okanye isixhobo esisetyenziselwa uhlalutyo.
Ndilulawulo olusemgangathweni kwi-HPLC Vials?
Yenza iimvavanyo zokulawula ezifana nokuvavanywa ngokuvuza kunye novavanyo loxinzelelo.
Zithini iimvavanyo zolawulo lomgangatho we-HLLC? Iinkalo ezili-13
Iimvavanyo zokulawula umgangatho eziqhelekileyo ziquka iimvavanyo zokuvuza, iimvavanyo zoxinzelelo, kunye neemvavanyo zokuhambelana.
1. Ukuchaneka kweQinisekisa ubungakanani be-vials, ukuqinisekisa ukuba bahlangabezana nemigangatho emiselweyo, enjengomhlaba wangaphakathi nowangaphandle, ubude kunye nentambo igqityiwe.
2. Umahluko wobunzima:Jonga ukufana kwesisindo se-vial ukuqinisekisa ubungakanani besampulu kunye nenaliti.
3. Ubunzima be-Wall:Linganisa ubukhulu beendonga ze-Vial ukuqinisekisa ukuba bayayifezekisa imigaqo-nkqubo efunekayo, enokuchaphazela ukuqina kunye nengqibelelo yesampulu.
4. Umgangatho weThrico:Vavanya intambo yokugqiba ukuchaneka, ukuqinisekisa ukutywina okufanelekileyo ngeCAPS kunye nokunciphisa umngcipheko wongcoliseko lwesampulu.
5. Iglasi icocekile:Vavanya ukuqulunqwa kweglasi ukuba ubunyulu kunye nokungabikho kokungcola okunokuphelisa iisampulu.
6. Umgangatho womphezulu:Vavanya i-vial yangaphakathi kunye nendawo engaphandle yeziphene, ukukrala, okanye amasuntswana anokuphazamisa ingqibelelo okanye uhlalutyo.
7. Ukuxhathisa kwamachiza:Vavanya ukuxhathisa kwe-vials kwiinyikima eziqhelekileyo kunye neeRegentnts ezisetyenziswe kwi-HPPC ukuqinisekisa ukuba abasabi senzekile okanye bangcolise iisampulu.
8. Ukunyaniseka:Jonga umgangatho we-Septa kunye nentsebenzo yokuqinisekisa ukuba badala itywina elithembekileyo ngaphandle kokuvuza okanye ukuphola kwesampulu.
9. Uvavanyo lokuvuza:Yenza uvavanyo lokuvuza ukuqinisekisa ukuba i-vials igcina itywina elifanelekileyo phantsi koxinzelelo okanye iimeko ze-vacuum, ukuthintela isampulu yesampulu okanye ungcoliseko.
10. I-cap yokuthengisa i-cap:Vavanya i-CAPS 'Ukwazi ukutywina, ukuqiniseka ukuba bayenza itywina nelitywili elivuzayo xa lisetyenziswa kwi-vials.
11. Ucoceko oluqinisekisiweyo:Qinisekisa ukuba i-vials icocwe kwaye ipakishwe kwindawo ecocekileyo yokuthintela ungcoliseko ngaphambi kokusetyenziswa.
I-12. Ukungqinelana kwe-batch:Vavanya ukungqinelana kwee-vials ngaphakathi kwe-batch yemveliso ukuqinisekisa umgangatho kunye nentsebenzo.
13. Ukuhlanjwa:Kwiimeko apho kufuneka khona ii-vials eziphalazelayo, qinisekisa ukuba ii-vials zichithwe kwaye zigcine ukugula kwazo de zisebenzise.
Ndazi njani ukuba i-vials yam ye-HPPC inomgangatho olungileyo?
Khetha i-vills ye-HPPL evela kumthengisi ongenakuphikiswa kwaye wenze iimvavanyo zolawulo lomgangatho.
Ndingakuqinisekisa njani ukuhambelana kwe-vials yam ye-HPPC ngesixhobo sam?
Nxibelelana nencwadana yezixhobo okanye uqhagamshelane noMvelisi wesixhobo wokufumanisa ukuba ulungelelwaniso lwe-viss ye-HPLC ngesixhobo.
Ndingaqinisekisa njani ukungangqinelani kwe-vials yam ye-HPPC ukusuka kwi-batch ukuya kwi-batch?
Khetha i-vills ye-HPLC evela kumthengisi ongenakuphikiswa kwaye wenze uvavanyo lolawulo lomgangatho kwi-batch nganye.
ISAHLUKO 6. Ukuthenga i-vialc vials
Ithini ixabiso le-HPPC?
I-HPLC (i-chromatography ye-chromatography) yamaxabiso e-vial inokwahluka kakhulu kuxhomekeka kwizinto ezininzi. I-villa esemgangathweni ye-HLLC ngaphandle kweempawu ezizodwa okanye iziqinisekiso ngokubanzi zixabisa malunga ne- $ 0.10- $ 2. Nangona kunjalo, ezo zinto zenzelwe ngokukodwa ukuhlangabezana nezicelo okanye iimfuno ezisemgangathweni zinokubiza naphi na ukusuka kwi- $ 2-10 + ngokuxhomekeke kwixabiso lezinto ezibonakalayo, ngenxa yoko kunobuchule bokubonisana neemfuno zakho kunye nezinto ozithandayo.
Ndingayithenga phi i-vials ye-HPPL?
I-HPLC Vials inokuthengwa kwiinkampani zokubonelela amalabhoratri okanye abathengisi abakwi-intanethi.
Zeziphi izinto endifanele ndiziqwalasele xa kuthengwa i-HLPC Vials?
Cinga ngexabiso, umgangatho, kunye nokuhambelana nesicelo sakho xa uthenga i-vialc.
Ndiwathelekisa njani amaxabiso e-vills ye-HPLC evela kubathengisi abahlukeneyo?
Thelekisa ixabiso leyunithi nganye kunye neendleko zokuhambisa ezivela kubathengisi abahlukeneyo.
Ndivavanya njani umgangatho we-HPLC evela kubathengisi abahlukeneyo?
Khetha i-vills ye-HPPL evela kumthengisi ongenakuphikiswa kwaye wenze iimvavanyo zolawulo lomgangatho.
Ndikhetha njani umthengisi onokuphindaphindiweyo we-HPLC?
Khetha umthengisi ngodumo oluhle, uphononongo olululo, kunye nembali yokubonelela ngeemveliso ezisemgangathweni.
Ukuququmbela, I-HPLC Vials yinto ebalulekileyo kuyo nayiphi na i-chromatography ye-chromatography. Ngokuqonda iziseko ze-HPLC, ukukhetha uhlobo olufanelekileyo lwe-vial, luzisebenzisa ngokuchanekileyo iingxaki eziqhelekileyo, ukwenza iimvavanyo zolawulo olusemgangathweni, kunokuqinisekisa kubathengisi abafanelekileyo, unokuqinisekisa iziphumo ezichanekileyo kuhlalutyo lwakho lwe-HPPC.
Nxibelelana nathi ngoku
Ukuba ufuna ukuthenga I-HPLC VialsI-AijIren, nceda unxibelelane nathi ngezi ndlela ezintlanu ezilandelayo. Siza kukuphendula ngokukhawuleza.
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