Ekuhlaziyweni kwe-HPLC, usayizi wezinhlayiyana we-Chromatographic Colog Packing mncane futhi kulula ukuvalwa yizinhlayiya zokungcola. Ngakho-ke, amasampula nama-solvents kudingeka ahlungwe kusengaphambili ukuze asuse ukungcola okungcolile futhi avikele insimbi. I-ION Chromatography, evame ukusetshenziswa ekuhlaziyweni kwemvelo, nayo idinga ukuthi akukho okungcoliswa kokungcola okungabonakali okwethulwe ekunciphisweni kwesampula. Izihlungi zesirinji zingasetshenziswa ekuhlaziyweni kwe-HPLC kanye nokuhlaziywa kwe-IC ukuthola izixazululo zesampula, okuyisinyathelo esibalulekile kwinqubo yokuhlekisa yesampula.
