Aug. 20, 2024
Yakakura Performance Liquid chromatography (HPLC) ishandare yakashandiswa imbudzi inoongorora inotora basa rakakosha muminda yakati wandei kusanganisira misheni, uye kuchengetedza kwezvakatipoteredza, uye kuchengetedzwa kwekudya. Nekudaro, kubudirira kweHPLC ongororo kunoenderana zvakanyanya pane kunaka kwekuenzanisa. Kugadzirira kwakakodzera kwekuenzanisa kunoenderana nekureba kwechokwadi kweanenge aongorora uye kuderedza kupindira. Ichi chinyorwa chakazara chinovhara zvese zvaunoda kuziva nezve HPLC semuenzaniso kugadzirira, kusanganisira kukosha kwayo, maitiro, maitiro akanakisa, uye matipi ekugadzirisa matambudziko.
Unoda kuziva zvakawanda nezve zvikumbiro zve chromatography vials, ndapota tarisa ichi chinyorwa: 15 zvikumbiro zve chromatography vials muminda dzakasiyana
Kukosha kwekugadzirira kwekuenzanisa muHPLC
Kugadzirira kweMuenzaniso idanho rakaoma muHPLC kuongororwa kwehuwandu hwezvikonzero.
Kuderedza kupindira:Sampuli dzinowanzove nehunhu uye matrix zvikamu zvinogona kukanganisa nekuonekwa kwekuongororwa kwekuongorora. Kubudirira Kwekutanga Maitiro Ekugadziriswa kunogona kubatsira kubvisa izvi zvisingadiwe zvinhu uye kuvandudza kuongororwa kwekuongorora.
Kuvandudza Ongorora Kudzoreredza:Kugadzirira kwakakodzera kunovandudza kuongororwa kwekuwana kupora uye kunozivisa kuti mhedzisiro inoratidza kusvinudzwa kweiyo yekutanga muenzaniso. Izvi zvinonyanya kukosha pakuongorora kwekuongorora uko fungiri zviripo pane yakaderera kutarisisa.
Yakavandudzwa Peak chimiro:Zvakakodzera ma samples anobatsira anobatsira kuumba manyoro uye akanyatsotsanangurwa mapeki mune chromatorm. Izvi zvakakosha kuti uwane ruzivo rwakakwana. Kugadzirira kwakashata kugadzirira kunogona kukonzera kupinda mumwekukura uye kuwedzeredza, kunoenderana nedudziro yedata.
Dzivirira yako HPLC system:Zvinosvibisa mumuenzaniso wako unogona kukuvadza makoramu uye zvimwe zvikamu mune yako HPLC system, inotungamira kuwedzera mari yekugadzirisa uye nguva yekugara. Kugadzirira kwakakodzera kwekuenzanisa kunoderedza njodzi yehurongwa hwekuvhara uye kupfeka.
Matanho Akakosha muHPLC Sample Kugadzirira
1. Muenzaniso muunganidzwa
Nhanho yekutanga mukugadzirira kwekutanga kuunganidza yako sampuro nenzira kwayo. Izvi zvinosanganisira:
Shandisa midziyo yakachena:Gara uchishandisa yakachena, inert midziyo kuti udzivise kusvibiswa. Girazi kana yakakwirira-mhando mapurasitiki midziyo inokurudzirwa.
Dzivisa kusvibiswa:Shandisa magurovhosi uye zvishandiso zvakachena kudzivirira kusvibiswa kubva kune sebum uye zvimwe zvinowanikwa.
Kunyora kwakakodzera:Zvakajeka zvinongedzo samples kudzivirira musanganiswa-ups uye uve nechokwadi chekutengeserana.
2. Sampule Homogenization
Sample Homogenization inosimbisa homogeneity, iyo yakakosha kumubereki mhedzisiro. Homogezation matekiniki anosanganisira:
Mechanical Homogenizization: Kusanganisa yakasimba kana semi-solid samples uchishandisa dhiza kana homogegenizer.
Ultrasonic homogenizization:Kushandiswa kweiyo Ultrasound kuparadzira zvimedu mukati mechikamu chemvura.
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Zvichienderana nezvakatarisirwa kusungwa kwekuongorora, samples dzinogona kunge dzinofanira kuiswa kana kuiswa. Izvi zvinogona kuwanikwa ne:
DILULItion:Dilute sampuli kune iyo yaunoda kusimbisa uchishandisa iyo yakafanana solvent seye mbozha chikamu.
Kusimbisa:Ongorora dzinogona kuongororwa uchishandisa maitiro akadai sekubuda kana kusimbisa chikamu chekubvisa (spe).
4. Kufefetera
Filtration yakakosha kubvisa zvinhu zvakaringana zvinogona kuvhara HPLC makoramu. Nzira dzakajairika dzekuputika dzinosanganisira:
Membrane Filtration:Shandisa0.2μm kana 0.45μm mafiritakubvisa zvimedu. Kazhinji polyethersulfone (PES) kana nylon mafirita anoshandiswa.
Prefiltration:Zvemasampuli ane yakakwira chikamu chemukati, preftration kuburikidza neakakura pore size size firita inogona kudzivirira kuvhara.
50 Mibvunzo inowanzo bvunzwa nezve syringe mafirita, ita shuwa yekuongorora ichi chinyorwa chinodzidzisa: Iyo musoro we "syringe filter" 50 mibvunzo inowanzo bvunzwa
5. Sampuli kugadzikana
Funga kuti kudzikamisa muenzaniso wako kuti udzivise kusvibiswa kwekuziva.
ph kugadzirisa:Kugadzirisa iyo pH inogona kubatsira kusimbisa mamwe ekuongorora. Semuenzaniso, maActic makomputa anogona kuda acidization yekudzivirira kusvibiswa.
Kuwedzera:Kuwedzera stabilizer kana kuchengetedza kunogona kubatsira kuchengetedza kutendeka kwemuenzaniso panguva yekuchengetedza uye kuongororwa.
6. Solvent Sarudzo
Izvo zvakakosha kusarudza iyo chaiyo solvent yekunyungudza yako sampula uye ive nechokwadi chekuita neyako HPLC system. Funga nezve zvinotevera:
Solvent Polarity:Kuti uwane kupaza kwakazara, solvent inofanira kuenderana nehurongwa hwehuwandu hwekuziva.
Nhare chikamu kuenzanisa: Shandisa solvent iyo inoenderana neye mobile chikamu kuti uderedze zvinogona kukanganisika.
7. Kugadzirira zviyero
Kugadzirira kwemari yeCalibration yakakosha kuti udzikise kuongororwa mune sampuli. Izvi zvinosanganisira:
Kugadzirira kweCalibration curve:Gadzirirai nhevedzano yekuzivikanwa kwekuzivikanwa kwekuziva kuti ivire calibration curve yehuwandu.
Shandisa iyo yakafanana solvent:Kuti uchengetedze kusawirirana, gara uchigadzirira zviyero zvako uchishandisa iyo yakafanana solvent uye mamiriro ezvinhu seyako samples.
Zviitiko Zvakanakisa zveHPLC Sampule Kugadzirira
Tevedza Standard Inoshanda Maitiro (SOPS):Simbisa uye kuomerera kumasonheji yekuenzanisa kwekuenzanisa kuve nechokwadi chekuenderana uye kubereka.
Shandisa yakakwirira-mhando reagents uye solvent:Gara uchishandisa HPLC-giredhi soles uye yakakwirira-yekuchena reagents kuti ideredze kusvibiswa.
Chengetedza yako Workspace Yakachena:Chengetedza yako yekugadzirira yekugadzirira nzvimbo yakachena uye yakarongedzwa kuderedza njodzi yekusvibiswa.
Nyora zvese:Chengetedza zvinyorwa zvakadzama zvemafungiro ako ekugadzirira maitiro, kusanganisira chero kutsauka kubva ku standard protocol.
Inosimbisa nzira:Simbisa nzira dzekugadzirira nguva dzose kuti uve nechokwadi chekuti vanopa akavimbika uye mubereki mhedzisiro.
Kugadzirisa dambudziko zvakajairika sampu yekugadzirira matambudziko
Murombo Peak chimiro:Kana mapundu akapamhama kana muswe, izvi zvinogona kuratidza kusakwana kwemukati kana kuvapo kwezvinhu zvechikamu. Ita shuwa kuti sampu yako yakanyatsosanganiswa uye yakasviba.
Ghost Peaks:Ghost Peaks inogona kukonzerwa nekusvibiswa mune solvent kana sampuli, shandisa yakakwirira-yekuchena soles uye simbisa kuti ese egirazi nemidziyo yakachena.
Kunzwa kwakaderera:Kana kunzwa kuderera kwakaderera, funga kuti optimizing nzira yako yekubvisa kana kuwedzera kusunganidzwa kwekuziva kwako.
Column Clogging:Kugara uchigura kunogona kuratidza kuti sampu yako ine akawanda akanyanya kugadzirwa kana kusiri kusekwa zvakakwana. Ongorora-kuongorora yako yekuyedza maitiro uye funga nezve-pre-filtration.
Migumisiro isingaenderane:Kusiyana mumhedzisiro kunogona kutsva kubva mukugadzirira kusapindirana. Gadzirisa maitiro ese uye uve nechokwadi kuti vashandi vanobatanidzwa mukugadzirira kwemuenzaniso vanodzidziswa zvakanaka.
Advanced Sampule Kugadzirira Maitiro
Sezvo ongororo dzekuongorora dzinowedzera, marebarendi ari kutora zvakanyanya sampuli yekugadzirira maitiro ekugadzirisa maitiro uye kunyatso:
1. Solid phase yekubvisa (SPE)
Spe is all shandiswa maitiro anobvumidza iyo yekusarudzika yekubvisa yekuongorora kubva ku yakaoma matrices. Zvinosanganisira kupfuudza sampuli yemvura kuburikidza neyakaipisisa adsorbent zvinhu izvo zvinokonzeresa ana ana ana kana vachibvumira kusvibiswa kuti vapfuure.
2. Liquid-mvura yekubvisa (lle)
LLL ndeye nzira iyo inoparadzanisa yekuongorora inoenderana neyavo solubility mune maviri asina kujairika zvinwiwa. Iyi nzira inoshanda mukuparadzanisa makomputa nehurombo hwakasiyana uye hunowanzoshandiswa mukuongororwa kwezvakatipoteredza uye nemishonga.
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Quechers inzira yakakurumbira yekugadzirira chikafu uye nharemamire yemagariro inosanganisa kubviswa uye kuchenesa nhanho kuita maitiro imwe, zvichiita kuti zvive zvakanaka uye zvinodhura.
4. Automation
Kuburikidza uye kubereka kunogona kuve kwakagadziridzwa zvakanyanya ne otomatiki yekugadzirira masisitimu, iyo inogona kuita mabasa, kupera kwesarudzo, uye pfus nekupindira kwevanhu kushoma, nekudaro kuderedza njodzi yekukanganisa.
Unoda kuziva zvakawanda nezveHPLC Sampula, ndapota tarisa chinyorwa ichi: HPLC yemuenzaniso yekugadzirira mhinduro dzemhedzisiro yakanaka
Mhedziso
HPLC yemumuenzaniso kugadzirira inhanho yakaoma iyo inogona kukanganisa zvakanyanya kurongeka uye kuvimbika kwemiongorori. Nekutevera maitiro akakodzera, uchishandisa maitiro akakodzera, uye kugadzirisa matambudziko akajairika, mabara anogona kuongorora maitiro ekugadzirira nekutora maitiro epamberi uye matekinoroji sezvinoda kuongororwa zvinoitika. Quustomation ichaenderera mberi nekuwedzera kushanda uye kushanda kweHPLC inoongorora. Kugadzirira kwakakodzera kwekugadzirira hakungovandudza kunaka kwemhedzisiro yemhedzisiro, asi zvakare inodzivirira iyo HPLC System, ichiratidza kuvimbika kwenguva refu uye kuita.
Chirungu
ChiChinese
