Ndeipi basa remitambo autosamupler muLC-ms kuongorora?

Kunzwisisa Autosamppler Vals

Autosamupler vials madiki midziyo yakashandiswa kubata samples yakasvitswa muLC-ms system yekuongorora. Aya mairi anogadzirwa kuti akwane mu quinopler, ayo otomatiki anongova vhoriyamu chaiyo yemuenzaniso mune chromatography system. Sarudzo yeVial inogona kukanganisa zvakanyanya kuita kweLC-ms kuongorora, kunokanganisa zvinhu zvakadai sekutendeseka, kusvibiswa, uye kubereka.

Vhura zvakavanzika zvechromatography vial gadziriro yekugadzirira chaiyo uye yakavimbika kuongororwa mumatanho matanhatu ari nyore. Verenga pane tenzi maitiro ehunyanzvi! Matanho matanhatu kugadzirira chromatography vials yekuongorora

ZVINOKOSHA ZVINOKOSHA zveAutosamppler Vals

SAMASE Midziyo:Autosamupler vals inobata samples zvakachengeteka kudzivirira kusvibiswa uye kubuda kwemvura. Dhizaini yeVial inovimbisa kuti muenzaniso unoramba wakasimba usati jekiseni muLC-ms system.

Kuenderana:Iyo vial inofanirwa kuenderana neye autosamupler uye lc-ms system. Izvi zvinosanganisira kufunga kwakadai saizi saizi, mitsipa yemitsipa, uye kuenderana kwezvinhu zvine solvent uye ongororo.

SAMPLA TERTE:Izvo zvinyorwa zvemusiyano zvinogona kukanganisa kugadzikana kwekuenzanisa. Semuenzaniso, mamwe mapurasitiki anogona leach makomputa mumuenzaniso kana kutora fani kuongorora, zvichitungamira kune mhinduro isingaenzaniswi. Magirazi magirazi anowanzo sarudza nekuda kwekuita kwavo.

Kurerutsa Kushandiswa:Iyo vial inofanira kunge iri nyore kubata uye kutakura mu autosampler. Zvimiro zvakadai sehuwandu hwekuyera ndima uye nzvimbo dzakajeka dzekushandisa dzinowedzera kusimudzira kusimudzira.

Mhando dzeAutosamppler Vals

Kune akati wandei marudzi emhando dzekutendeuka dzekutendeuka kuti dzisarudze kubva, imwe neimwe yakagadzirirwa kuti zvikumbiro uye zvinodiwa:

1. Mart mauto

Mauto miganhu ndiyo inonyanya kushandiswa mhando muLC-ms kuongorora. Ivo vanowanzogadzirwa negirazi kana mapurasitiki emhando yepamusoro uye vanouya mumhando dzakasiyana siyana, dzakadai se 1 ml, 2 ml, uye 4 ml, 4 ml Aya mairume akakodzera kune dzakasiyana siyana dzekushandisa uye dzinoenderana nevakawanda autosample.

2. Screw cap vials

Screw cap vialsuve nemutsipa wakashongedzwa unosimbisa zvakachengeteka ne cap. Dhizaini iyi inobatsira kudzivirira kubuda uye kusvibiswa. Screw cap vials yakanakira kusimbisa samples kana samples dzinoda kuchengetedza kwenguva refu.

3. Crimp yepamusoro vials

Crimp yepamusoro vialsvanoiswa chisimbiso neAluminium Crimp Caps iyo inopa chisimbiso chemagetsi, kunyanya kune mweya-inonamira samples. Aya maviru anowanzoshandiswa kumasampuli anodikanwa kugadzikana uye kuchengetedzwa kubva mukushatiswa.

4. Nyaradzo vials

Varume vane hunyanzvi vakagadzirirwa kuti vanyaradzwe zvikumbiro, senge izvo zvinoda pre-sampu yekugadzirira kana ine fungidziro yekuongorora. Semuenzaniso, vials vane mafirita akabatanidzwa vanogona kubatsira kubvisa zvimedu zvisati zvaitika, nepo mavheti ane zviyero zvakati wandei anogona kuderedza adsorption yekuongorora.

Unoda kuziva nzira yekusarudza crimp vial vs. snap vs. screw cap vial ?, Tarisa chinyorwa ichi: Crimp vial vs. snap vial vs. screw cap vial, maitiro ekusarudza?

Kukosha kweMuial kusarudzwa muLC-ms kuongorora

Kusarudza iyo chaiyo autosamppler vial inokoshesa kuwana chaiyo uye yakavimbika lc-ms zvinobuda. Hezvino zvimwe zvinhu zvakakosha zvekufunga kana uchisarudza vial:

1. Kemical kuwirirana

Izvo zvinyorwa zveVial zvinofanirwa kuenderana neiyo solvent uye kuongorora kuongororwa. Magirazi magirazi anowanzove ane basa uye akakodzera kune akawanda mashandisirwo, nepo mamwe mapurasitiki anogona kudyidzana nemafuta kana ekuongorora, zvichikonzera kusvibiswa.

2. Muenzaniso vhoriyamu

Iyo vial inofanirwa kubata huwandu hweyenyepedzeri hunodiwa kuti uongororwe. Vaviri vials vanowanzobata 1 ml kusvika ku5 ml, asi zvimwe zvinoshandiswa zvinogona kuda yakakura. Kuverengera kuti saizi yeVial yakakodzera kune iyo jekiseni vhoriyamu zvinodiwa zve autosampler inokosheswa.

3. Kuvhara vial

Iyo yekuvharwa kwemuchina yeiyo vial inogona kukanganisa kutendeka kwemuenzaniso. Screw cap uye Crimp cap vials inopa sarudzo dzakasiyana dzekuisa chisarudzo, uye sarudzo inoenderana nekusagadzikana uye kusimbisa zvinhu zvemuenzaniso. Nenzira yakakodzera kuisimbisa vial inodzivirira kubuda nekuzvisvibisa panguva yekuchengetera uye kubata.

4. Vial kuchena

Autosamupler valsInofanirwa kuve yakachena uye isina kusvibiswa izvo zvinogona kukanganisa kuongororwa. Inokurudzirwa kushandisa vials yaive pre-yakanatswa uye yakasimbiswa yeLC-ms application kuti ive nechokwadi chekutendeka.

5. Mutengo uye kuwanikwa

Nepo mhando yakakosha, mutengo uye kuwanikwa zvakare kutamba chiitiko mune vial kusarudzwa. Laboratori dzinofanira kuyera kukosha kwemhando yepamusoro-makompiyamo ane quentary fungidziro uye kuve nechokwadi chekuti vigics yakasarudzwa inowanikwa nyore nyore kushandiswa kwekuenderera mberi kwekushandiswa kuri kuitika.

Maitiro akanakisa ekushandisa autosamppler vials muLC-ms

Kuve nechokwadi chekuita kwakakwana muLC-ms kuongorora, funga izvi zvinotevera zviitiko zvekushandisa autosamupler vals:

1. Kubata kwakakodzera uye kuchengetedza

Kubata vials nokungwarira kuti udzivise kusvibiswa. Pfeka magurovhosi kana kubata vials uye samples kuderedza njodzi yekuunza zvinosvibisa. Chitoro makurukota munzvimbo dzakachena, dzakaoma dzekudzivirira kusvibiswa kwemuenzaniso.

2. Shandisa iyo yakakodzera vial mhando

Sarudza iyo yakakodzera vial mhando zvichienderana nezvinodiwa zvekuongorora. Kune masampuli akasimba, funga nezvekushandisa chisimbiso cheScrew cap kana crimp yepamusoro vials. Yemasampuli anoda kupera kwefaira, shandisa vials ine mafirita akabatanidzwa.

3. Kugara nguva nenguva yekugadzirisa

Chengetedza uye calibrate iyo autosampler nguva dzose kuti ive nechokwadi chechokwadi uye chinowanzo simbisa majekiseni. Suka autosamupler zvikamu, kusanganisira tsono uye jekiseni chiteshi, kudzivirira kuyambuka-kusvibiswa pakati samples.

4. Optimize sampule vhoriyamu

Ita shuwa kuti vhesi yemuenzaniso yakakodzera kune iyo jekiseni yekugona kweiyo autosampler. Kuwedzeredza kana kuiswa musial kunogona kutungamira ku jekiseni zvikanganiso uye kukanganisa hunhu hwekuongorora.

5. Gwaro Remumu Rokugadzirira Maitiro

Chengetedza zvinyorwa zvakadzama zvemaenzaniso ekugadzirira maitiro, kusanganisira kusarudzwa kwechienzi, sampuli vhoriyamu, uye chero shanduko yakagadzirirwa kune yakajairwa protocol. Chinyorwa ichi chakakosha kubereka uye kutevedzera zviyero zvekudzora.

Wana kuziva ruzivo rwakazara nezve nzira yekuchenesa iyo chromatography sampulies, ndapota tarisa chinyorwa ichi: Inoshanda! 5 nzira dzekuchenesa chromatography sampuli

Mhedziso

Autosamupler vials vanotamba chinzvimbo chakakosha mukubudirira kweLC-ms kuongorora. Kugadzirwa kwavo, kupindirana kwezvinhu, uye kusimbisa mashandisirwo zvakananga zvinokanganisa muenzaniso kutendeka, kusvibiswa njodzi, uye maitiro anoongororwa. Nekunzwisisa kukosha kwesual sarudzo uye kuomerera kune zvakanakisa maitiro, mabharari anogona kusimudzira kuvimbika uye kurongeka kweiyo lc-ms yavo inoongorora. Sezvo kudikanwa kwekunyepedzera uye kuongororwa kwehunyanzvi kwekuongorora kunoenderera mberi nekukura, iyo autosamupler vials icharamba ichikosheswa mukuita kuti uwane kunaka kwemhedzisiro dzakasiyana-siyana mukutsvagisa uye indasitiri.