E loketsengBoitokisetso bo boholoe bohlokoa ho fumana li-chromograph tsa li-chrotographic tse nepahetseng. Sena se ile sa ngola lintlha tse tšeletseng tsa bohlokoa tseo ba li batlang li tiisa ho boloka botšepehi bo ka ba teng ho pheha khang ea lekhetho bakeng sa ho hlahlojoa. Ka ho utloisisa mohato o mong le o mong oa mohato ka mong, ho latela mekhoa e metle, Li---ratoeries li ka fokotsa tšilafalo ha li ntse li eketseha tsa mohlala ha lihlahlobo tse molemo haholoanyane tse fetang tebello.

Pretory ea li-vielis pele ho li-chromagraphic tsa bohlokoa li le bohlokoa molemong oa ho beha mabaka a tšepahalang le a sirelletsang ho fetoha ha tsona.

Mohato oa 1: Ho khetha mofuta o loketseng oa Via

E le karolo ea boitokisetso ba vaele, mohato oa pele o lokela ho kenyelletsa ho khetha ntho e loketsengMofuta oa ViaHo ipapisitsoe le lintlha tse kang molumo oa mohlala le ho tsamaisana le mekhoa ea ho itlhahloba. Tlhokomelo e boetse e lokela ho nkoa ha o khetha likhetho tse koalang ho netefatsa tiiso e sireletsehileng khahlano le likheo kapa tšilafalo.

Mohato oa Bobeli: Ho hloekisa le ho hlatsoa likotlo

Ho hloekisa ka botlalo mmeho hlatsoa lipitsaHo hlokahala ho tlosa masala kapa balumeli ba neng ba ka kena-kenana le li-solvel tse fapaneng ho hloekisa le li-solvel li lokela ho khethoa ho latela litaba tsa motho ea ikhethileng. Mohato ona o thusa ho boloka mohlala oa ho itlhakisoa ha ke ntse ke thibela tšilafalo ea sefapano.

Mohato oa 3: Ho hlokahala hore ho be le bolokolohi ba Teko

Pele a sebelisa, ho bohlokoa haholo hore likhama tse hlahlobeloang ka hloko bakeng sa mapetso, lichifi kapa bofokoli bo ka sekisang botšepehi ba bona. Ts'ebetso e koaloang e lokela ho netefatsoa ho netefatsa tiiso e thibelang mothapo le e sireletsehileng e thibelang tšabo ea nako le ho hlahlojoa.

Mohato oa 4: Ho lokisa lithaelese tsa mohlala

Ho itukisetsa hantle le ho sebetsana le tharollo ea mohlala ke ntho e bohlokoa ho hlahlojoa e nepahetseng. Bohato bona bo kenyelletsa ho latela li-Protocol tse nepahetseng ha ho etsoa tharollo ea mohlala, ho ba le li-solvets tse ka bang teng le ho khetha li-solvents tse ke keng tsa fetola thepa ea bona ea lik'hemik'hale nakong ea nako.

Mohato oa 5: Ho tlatsa li-vial ka sampole

Ho tlatsa haholo le ho khotsofatsa haholo ho bohlokoa tlhahlobong e tšepahalang. Mekhoa ea ho fumana liphallelo kapa lits'ebetso tse ikemetseng li lokela ho sebelisoa ho netefatsa hore mehlala e lekanang ea mohlala e kholo; Ho feta tekano kapa ho sa tsitsa ho ka bakoa liphetho tse sa nepahalang, ka hona, tekanyo e nepahetseng e lokela ho bolokoa ka linako tsohle.

Mohato oa 6: Ho ngola le ho boloka

Ho ngola lipaleKa tlhaiso-leseling ea bohlokoa, ho kenyelletsa le tsebiso ea mohlala le letsatsi, ke ea bohlokoa bakeng sa ho bolokoa le litokomane. Ho feta moo, maemo a polokelo - ho kenyelletsa le tsamaiso ea mocheso le ts'ireletso e bobebe - e tlameha ho ba e phethahetseng ho netefatsa maemo a botsitso.

Matla a netefatso ea boleng nakong ea ho itokisa ka Vial

Ho boloka litekanyetso tse phahameng tsa boleng ba boleng, li bohlokoa haholo bakeng sa lits'ebetso tse tloaelehileng tsa ts'ebetso (sops) li hlokometsoe ts'ebetsong ea seemo, ho nepahala ho netefatsa lits'ebetso tsa ho hlahloba.

Mathata a tloaelehileng a tokiso le tharollo

Ho itokisetsa ho fana ka mathata a bophelo bo ikhethang, ho kenyelletsa ho thata ho tlosa masala kapa likotsi tse ka hlahisang tlokotsi. Maano a kang ho sebelisa li-agents tse ikhethang le ho fokotsa ho ts'oaroa le ho pepeseha ho ka thusa ho netefatsa boitokisetso bo matla ba ho etsa tokiso e matla.

Ho hloekisa le ho hira mekhoa ea ho hloekisa le ho itokisa

Tsoelo-pele eaho hloekisaMme mekhoa ya ho itokisa e kenyelletsa baemeli ba ho hlatsoa litšila le mahlale a ntlafalitsoeng, ka bobeli ba bona ba ka ntlafatsang katleho, e feto-fetohang le katleho ea boitokiso ba eona bo emeng.

Leqheka la Via ho Tlhahlobo

Motsoako oa vaeele o bapala karolo ea bohlokoa ho sekaseka tlhahlobo. Lintlha tse kang karohano ea lik'hemik'hale, mohlala, ho ba le botsitso ba lik'hemik'hale pakeng tsa sampole le lisebelisoa tsa valia ho tlameha ho hlahlojoa bocha ha bo khetha sebopeho se setle.

Ho ntlafatsa boitokisetso sa mohlala bakeng sa mekhoa ea ho sekaseka

Mekhoa e fapaneng ea tlhahlobo e kang hplc,GC, kapa tlc e ngoe le e ngoe e na le litlhokahalo tse tobileng tsa boitokisetso ba ho itokisa tse hlokang ho khoptjoa ho fihlela sephetho se nepahetseng le liphetho tse hlakileng. Ka hona, ho sebetsana le ts'ebetso ea theko e boima ea Viane le sesebelisoa ka seng le mokhoa o sebelisitsoeng ho fihlela sephetho se atlehileng le se nepahetseng.

Ho fana ka maikutlo le ho thulana

Karolo ea bohlokoa ea ho ikoetlisa le ho hlahisa bokhoni nakong ea lekhetho ea lefapha ka nepo ke litokomane tse nepahetseng tsa ts'ebetso mabapi le ho ngola mekhoa ea ho hloekisa, ho iphetola ho tsoa ho protocols e tloaelehileng.

Ho ngola lintlha tsena li tiisa hore ts'ebetso ea bohlokoa ea boitokiso e ka tsamaisoa hantle kapa e boletsoeng haeba ho hlokahala.

Koetliso le Thupelo ea ho Tsamaisa Litokelo tsa Via

Koetlisa basebetsi ba laboratori ka mokhoa o boima le ho itukisetsa ho tiisa hore ba utloisisa mohato o mong le o mong mme o latela mekhoa e nepahetseng. Mananeo a koetliso a lokela ho koahela lihlooho tse kang khetho e tenang,Tharollo ea sampoleBoitokisetso, Mehato ea taolo ea matla le mekhoa e netefatsang ea boleng. Basebetsi ba ho tsebisa litho tsa setso sa mekhoa e metle ho ba thusa ho lula le ho fetoha le ho nepahala ho fihlela lits'ebetso tsa lihlahla.

Ho hlola Liphoso Tse Tloaelo le Marola a Vialiction

Ho utloisisa liphoso tse tloaelehileng le likotsi tse amanang le ho itokisa le ho tiisa lipalesa, tse kang ho hloekisoa kapa ho itlhoekisa ka mokhoa o sa lekaneng kapa tse sa nepahalang. Ka ho utloisisa maraba ana a ka bang teng, li-laboratories li ka sebelisa mehato ea khalemelo le ho netefatsa botšepehi ba ts'ebetso ea bona ea ho itokisa.

Melao le litaelo tsa indasteri ea indasteri bakeng sa Vial Prex

Ho latela melao le litataiso tsa indasteri ea indasteri ho bohlokoa ha u pheha tse veele tsa ho pheha khang ea ho sekaseka. Litokelo tse fapaneng tsa tsamaiso le litlhokahalo tsa indasteri le litlhokahalo tse ikhethileng li bontša litlhokahalo tse ikhethang tsa boitokisetso ba lefatše, joalo ka lits'ebetso tsa ho hloekisa, mekhoa ea litokomane le mehato ea ho laola mekhoa ea ho laola mekhoa ea boleng. Ho khomarela melao ena le litataiso ho thusa lipalesa tse phahameng tsa boleng le ho nepahala ha likomiti tsa bona tsa ho sekaseka.

Ho hloka tsebo le mekhoa ea ho itokisa

Litokisetso tsa Velical li ntse li tsoela pele ho fetoha le tsoelo-pele ea lisebelisoa le ho koaloa, joalo ka ho ntlafatsoaThepa ea Mefutale theknoloji ea pele ea ho hlophisa lintho tse boima. Tsoelo-pele e joalo e fana ka mekhoa e tšepahalang le e tšepahalang ebile e mela ea mekhoa ea Valtions Valtion.

Litefiso tsa Liabelo tsa Bohlokoa tsa Ts'ebetso ea Li-laeboratories tsa Tlhahlobo

Ha li-laboratories tsa ka ha li hlahlojoa li ka rua molemo haholo ka ho ithuta lithuto tsa visano e atlehileng ea boitlamo ba ho itokisa ka laboratori. Maemo a joalo a fana ka mehlala ea 'nete ea bophelo le ho bonahatsa phello ea bona mabapi le litlhahiso, ha a ntse a bontša mekhoa ea ho sekaseka, le ho fana ka tharollo e sebetsang le ho fana ka tharollo e sebetsang ea mathata a tloaelehileng. Li-labs tsa ka ha li hlahlobisisa li ka ithuta linyeoe tse joalo re sebelise lithuto tsena ho matlafatsa lits'ebetso tsa bona tsa boitokiso.

Mekhoa ea nako e tlang ea ho itokisa bakeng sa tlhahlobo

Lintho tse esale pele tse boletsoeng nakong e fetileng li fana ka maikutlo a hore ho lokisoe e tla tsoela pele le ho kopana ho eketsehileng ka mahlale a hlahang. E lebelletsoe litaelo tsa ka nako e tlang li kenyelletsa ho hlaolela mekhoa e metle ea ho hloekisa, likarolo tsohle tsa ho itokisa, ho sebelisa thepa ea liroboto tse ka phaelisang lits'ebeletso tsa boitokiso.

Sephetho

Ho itokisa lintho tse ngata ke seo ba se hlahlobang li-chrotographic, le li-laboratori li ka nka mehato e 'maloa tsa bohlokoa tse boletsoeng ho lits'ebeletso tsa tsona tsa bohlokoa. Ho khomarela mekhoa e metle, ho lula le metsamao le tataiso le tsoelo-pele ea kamoso e tla kenya letsoho ka mekhoa e metle ea ho itokisa.

Ela hloko

Ela hloko mehato ena ea bohlokoaMalinyane a chrotographyHo itokisetsa: Ho utloisisa bohlokoa, ho khetha mekhoa e nepahetseng, ho hlahloba lintho, ho ithuta, ho ithuta ho fana ka litlhaloso, le ho lebella lintlha tse ling tsa ho ithuta le ho lebella lintlha tse ling.

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E fetileng:

Ho hlahloba baetsi ba etellang pele ba liphaephe tsa ciaromatography

E 'ngoe:

Bokamoso ba Liphate tsa Chromatography: mekhoa ea ho shebella ka 2023