HPLC e ntle ea HPSC ke eng?

I. Selelekela

HPLC, kapa e phahameng ea chromatography ea chromatography, ke mokhoa o sebelisoang haholo ke k'hemistri ea tlhahlobo ea tlhahlobo. Liphetho tsa HPLC li itšetleha ka lisebelisoa tse sebelisoang, ho kenyelletsa le li-vilias tse tšoereng disampole. Bakeng sa liphetho tse nepahetseng le tse tšepahalang, ho bohlokoa hore u khethe le tokeloHpst vaal.

Sengoliloeng sena se tla totobatsa likhopolo tsa bohlokoahali ha a khetha li-veltic tse sa sebetseng. Re tla sheba mefuta e fapaneng ea li-valc vaianos le likarolo tsa tsona tsa sehlooho. Re tla fana ka litlhahiso mabapi le mokhoa oa ho khetha le ho sebelisa likhakanyo tsa HPLC bakeng sa ts'ebetso e nepahetseng le sephetho se nepahetseng.

II. Mefuta ea li-piplc tsa HPLC

Liphaelo tsa khalase ke mofuta o sebelisoang haholo oa HPLC, joalo ka ha a fana ka khatiso e ntle ea lik'hemik'hale, e leng ho hlaka, e etsang hore ba tšoanelehe likopo tse ngata. Lintho tse tloaelehileng tse sebelisetsoang ho hlekefetsoa ha li-Hplc ho bakarisose, ka lebaka la ho hanela tlhaselo ea eona ea mocheso le ea lik'hemik'hale. Li-vaels tsa khalase tsa Amber li loketse mehlala e metle le ho fana ka ts'ireletso e kholo ho tsoa lebone. Leha ho le joalo, ha ba phutholohe ho feta likhahla tse hlakileng tsa khalase.

Li-vavers tsa polasetiki le tsona li na le tlhoko e phahameng ea boima ba 'mele le tse theko e tlaase. Li-vials tsa polypropiplene li sebelisoa hangata. Li ka lumellana le li-solvent tse ngata tse mahlonoko empa eseng kaofela. Li entsoe ka polyethylene (pe), e loketseng polokelo le ho tsamaisa li-disampole tse tlase tsa lik'hemik'hale.

Lintho tse sebelisoang bakeng saHpst vaale khethoa ke mohlala oa mohlala, sistimi ea ho aloa le ho alafatsoa le mokhoa oa ligrama tsa chromatography. Ho qoba tšilafalo, ho bohlokoa ho netefatsa hore thepa ea vial e tsamaisana le solvents kapa reagents e sebelisoa.

III. Ho na le lintho life tse ntle tsa HPLC?

Ho se sebetse hantle ha li-HPLC Ho thibela tahlehelo ea mohlala nakong ea tlhahlobo, likhama tse lokelang li lokela ho lekana hantle le lisebelisoa le lisebelisoa tsa li-chromatography le lisebelisoa tse fumanehang joalo ka li-caps le Septa.

1.Compatationality ea li-solvet

Li-vael tsa Hplc li lokela ho lumellana le li-solvents tse sebelisoang bakeng sa li-chromatography tsa chromatography. Li-solven li ka nyenyefatsa thepa ea vae kapa li ka bolaeang lintho tse tsoang, tse ka amang liphetho le sampole. Li-vial tsa khalase li ka lumellana le li-solvents. Liphoofolo tse ling tsa polasetiki tse lumellanang le li-solvents tsa libaka tse pharaletseng lia fumaneha.

2. SOWLETES \ Letlaletso

Leqheka le ka lokollang metsoako e tsoang ho vaethe ho sampole ho ka lokolla mekhahlelo ho solvent. Ka bobeli li ka lebisa liphellong tse ntle kapa tsa bohata tse ka u thusang ho khetha li-vials tse nang le litlolo tse tlase \ letlapa. Li-vials tsa khalase li tsejoa ho na le maemo a tlase a li-axatctives \ lilakane ho feta litebele tsa polasetiki.

3.Cons

Ts'ebetso ea Tlhahiso ea HPLC Valias e ka ba le tšusumetso ts'ebetsong ea bona le ho ts'epahala ha litholoana tsa tlhahlobo ea kelello. U lokela ho reka feela likhama tse entsoeng ke baetsi ba nang le botumo bo botle bakeng sa taolo ea boleng. Ho bohlokoa ho khetha likhama tse tsoang ho babusi ba nang le botumo ba taolo ea boleng le ho latela melao ea indasteri.

4. Sisteme ea chromatography e tlameha ho tsamaisoa ka nepo kh'amera.

Hpst vaale fumaneha ka meralo e fapaneng ho kenyelletsa ho nyoloha holimo, ho chesa litlolo tse ngata. E mong le e mong o na le litšobotsi tsa hae tse ikhethang. Ha u khetha likhamallo, ho bohlokoa ho netefatsa hore li lumellana le sistimi ea li-chromatography hammoho le sistimi e tšoanang le li-caps le Septa. Li-vials li lokela ho lekana ka nepo ho netefatsa hore li tiisa hantle le hore ha ho sampole e lahlehileng nakong ea ho hlahlojoa.

Mesebetsi ea Lefatše la 2ml 9mm hplc e na le li-vaiare tse nang le sehlooho sena se bulehileng. Lekola likarolo tsa bona le likopo tsa bona ho sempele ea tlhahlobo ea tlhahlobo:1.5ml 9mm e khuts'oane e futsanehileng Veal ND9

IV. Ho sebetsana ka mokhoa o nepahetseng le ho boloka chelete ea li-hipc

Ho etsa bonnete ba litholoana tse nepahetseng le tse tšepahalang, ho bohlokoa ho sebetsana le li-wedc tsa HPLCC ka nepo. Mona ke mekhoa e seng mekae e metle eo u lokelang ho e nahana:

1. Qoba ho pepesetsoa ke leseli: leseli le ka nyenyefatsa mehlala e meng. Boloka literene libakeng tse lefifi kapa li li koahele li sebelisa lintho tse lefifi.

2. Li-vials li lokela ho bolokoa ka mocheso o nepahetseng oa mocheso. Boithene bo feteletseng bo ka boela ba ama boleng ba disampole.

3. Qoba mongobo. Mongobo ke sesosa se ka sehloohong sa tšilafalo. Ho bohlokoa ho boloka li-vials tikolohong e omileng.

4. Qoba ho fetola cap. Li-caps li ka phomola haeba li tiisitsoe haholo. Caps e tlameha ho e fihlela feela ntlheng ea ho theha tiiso.

5. Label liemahalle ka nepo: Ho qoba pherekano le ho netefatsa botsitso, ho bohlokoa ho ngoliseng mashano ka tsela e nepahetseng. Label vaialis ka ho hlaka ka lebitso la sampole, letsatsi, le tlhaiso-leseling e 'ngoe e nepahetseng.

6. Pele u sebelisa li-pieles, ho bohlokoa ho ba hlahloba bakeng sa tšenyo kapa bofokoli.

Ho bohlokoa ho sebelisa li-piele tse netefalitsoeng ho netefatsa hore li tsamaisana le sistimi ea li-chromatography le ho etsa lintlha. Ho sebetsana ka nepo le ho boloka lintho tse ngata tsa Hplc ho ka thusa ho qoba litšila 'me u hlahise litholoana tse nepahetseng le tse tšepahalang.

V. Mekhoa e metle ea tšebeliso ea li-vplc vial

Ho bohlokoa ho sebelisa li-velc tse phelisang hore li etse esale pele le ho fana ka liphetho tse nepahetseng. Li-vael tsa Hplc li lokela ho lula li theha mocheso le khanya ea letsatsi. Mongobo le ho pepesetsoa ke litholoana tse matla le sephetho sa boleng.

Sebelisa li-piele tse netefalitsoeng feela. Li-vial tse netefalitsoeng li lekiloe ka ts'ebetso le boleng. Liphoofolo tsena li ka sebelisoa bakeng sa mekhoa ea tlhaiso e hlokang ho nepahala le ho nepahala.

Label e liehelletsa ka nepo: Ho bohlokoa ho ngola ka nepo ka tlhaiso-leseling e amanang, joalo ka lebitso la mohlala, ho tsepamisa mohopolo. Sena se tla thusa ho qoba pherekano kapa liphoso ha u sekaseka.

Lekola li-vielis pele li sebelisoa bakeng sa tšenyo kapa bofokoli bofe kapa bofeto. Li-vaels tsohle tse nang le matšoao a ho senya kapa bofokoli li lokela ho lahloa.

Ho latela mekhoa ena e metle e tla thusa basebelisi ho netefatsa seoHpst vaale ka sebelisoa ka tsela e holisang ho nepahala le tšebetso ho li-corromatographs tsa chromatography.

VI. Mokhoa oa ho khetha li-pilc tse nepahetseng tsa HPLC

Ho bohlokoa ho nahana ka mofuta oa sampole le ntho e lumellanang le ea solt ha u khetha li-hilc tse ngata. Haeba sampole ke acidic, ho molemo ho sebelisa li-viellosate tsa korolicate, tse hanang ho hlasela ka lik'hemik'hale ho feta mefuta e meng ea khalase. Haeba sampole e le mapolanka e etsoa ho tsoa polypropylene kapa lisebelisoa tse ling tse nang le bokhoni bo tlase ba adsorgetion e kanna ea tšoaneleha hamolemo.

Budget ke lebaka le leng hape. Le ha li-vies tse ling li theko e boima ho feta tse ling, litšenyehelo tsohle tsa chromatography ha li angoe ke theko ea sesosa. Li-vaels tse sa lumellaneng kapa tsa boleng bo tlase li ka lebisa ho sa nepahalang tse sa nepahalang le tse theko e boima.

Li-pilip tsa Hplc li fumaneha ho li-Brand tse 'maloa tse tsebahalang, e leng e' ngoe le e 'ngoe ea tsona e na le likarolo le melemo ea eona. Metsi, alont thermo firmo le shimadzu ke tse ling tsa lihlahisoa tse tsebahalang haholo. Pele u reka, ho bohlokoa ho bapisa le ho etsa lipatlisiso tsa mabitso a fapaneng.

VII. Sephetho

Ho khetha ka nepoHpst vaalBakeng sa sistimi ea hau ea li-chromatography e bohlokoa ho fumana sephetho se nepahetseng le se tšepahalang. Ha u khetha li-hipls tsa hplc, liphihlelo tsa bohlokoa li kenyelletsa litaba tsa bohlokoa, tse lumellanang le litsamaiso tsa cromatography le mekhoa ea taolo ea matla. Bahlahlobi ba ka fihlela liphetho le ts'ebetso ka ho latela mekhoa e metle ha ba sebetsana le litšila, mongobo le ho ngola mahobe pele ho sebelisoa.

Ho na le likhetho tse ngata tsa li-HPLC tse ngata tse fumanehang. Ho bohlokoa ho bapisa le ho etsa lipatlisiso tsa matšoao a fapaneng ho khetha khetho e nepahetseng bakeng sa litlhoko tsa hau tsa tlhahlobo ea tlhahlobo. Bahlahlobisisi ba ka fihlela liphetho tse tšepahalang le tse lumellanang ka ho khetha mefuta e lumellanang le e lekaneng le ho latela mekhoa e nepahetseng le ho sebetsana le mekhoa e nepahetseng ea ho boloka.