Yonke into oyifunayo ukwazi malunga nokulungiselela isampulu ye-HPLC

Ukulungiswa kwesampulu linyathelo elibalulekileyo kuhlalutyo lwe-HPPC ngenxa yezizathu ezininzi.

Ncincisani iziphoso:Iisampulu zihlala zinezinto zokungcola kunye neMatrix ezinokuthi ziphazamise ekufumaneni uhlalutyi ekujoliswe kuko. Iindlela ezifanelekileyo zokulungiselela isampulu zinokunceda ekususeni ezi zinto zingafunekiyo kunye nokuphucula ukuchaneka kohlalutyo.

Phucula uhlalutyo kwakhona:Ukulungiselela ngokufanelekileyo kuphucula ukuhlalutya kwakhona kwaye kuqinisekisa ukuba iziphumo zibonisa eyona nto iphambili yesampulu yentsusa. Oku kubaluleke ngakumbi ekuhlalutyo kulo uhlalutyo apho uhlalutyo bekhona ngokuxinana okuphantsi.

Ubume be-Peak ephuculweyo:Iisampulu ezilungiselelwe ngokufanelekileyo zinceda iFomu ye-Symetrical kwaye ichazwe kakuhle kwi-chromatomgram. Oku kubalulekile ukuba kuchaneke ngokuchanekileyo. Ukulungiselela kwesampulu engafanelekanga kunokukhokelela ekutsaleni i-Peak Taiing kwaye kunyuke, nto leyo enza lula intetho yedatha.

Khusela inkqubo yakho ye-HPPC:Ungcoliseko kwisampulu yakho lunokonakalisa iikholamu kunye nezinye izinto kwiNkqubo yakho ye-HPLC, ekhokelela ekunyukeni kweendleko zesondlo kunye nexesha lokuphumla. Ukulungiselela kwesampulu efanelekileyo kunciphisa umngcipheko wokuvala kwenkqubo kunye nokunxiba.

Inyathelo lokuqala kwilungiselelo lesampulu kukuqokelela isampulu yakho ngokuchanekileyo. Oku kubandakanya:

Sebenzisa izikhongozeli ezicocekileyo:Soloko usebenzisa izikhongozeli ezicocekileyo, ze-inter ukunqanda ungcoliseko. Iglasi okanye iplastiki ekumgangatho ophezulu yeplastiki iyacetyiswa.

Khusela ungcoliseko:Sebenzisa iigloves kunye nezixhobo ezicocekileyo ukukhusela ungcoliseko ukusuka kwi-sebum kunye neminye imithombo.

Ilebheli efanelekileyo:Ngokucacileyo iisampulu zesampulu ukunqanda ukuxuba kunye nokuqinisekisa ukulandelela.

Isampulu ye-homogesish iqinisekisa i-homogeneity, ebaluleke ngokwesiphumo sokubuyiswa. Iindlela ze-homonizetion zibandakanya:

I-homogesical manya

I-Ultrasonic Homonises:Ukusetyenziswa kwe-ultrasound ukusasaza amasuntswana ngaphakathi kwesampulu engamanzi.

Kuxhomekeka kugxininiso olulindelekileyo lohlalutyi, iisampulu zinokufuna ukudilizwa okanye zijolise kuzo. Oku kunokufezekiswa:

I-dilution:Hlala isampulu yendlela enqwenelekayo usebenzisa isolvent enye njengenqanaba leselula.

Uxinzelelo:Uhlaziyo lunokugxila kusetyenziswa iindlela ezinje nge-Exvaporation okanye iSigaba seSigaba esiSiseko esiqinileyo (Spe).

Ukuqulunqa kubalulekile ukususa umcimbi wokubandakanya iikholamu ze-hplc. Iindlela eziqhelekileyo zokucoca zibandakanya:

I-MembraneSebenzisaI-0.2μm okanye i-0.45M yeifilithaukususa amasuntswana. Ngokwesiqhelo i-polyeherulfone (Pes) okanye i-filters ye-nylon isetyenzisiwe.

Ukulungiswa kwemali:Kwiisampulu ezinomxholo ophezulu ophezulu, ukwalathisa ngokuthintela ubungakanani bobukhulu obune pore


Imibuzo ebuzwa rhoqo malunga neefiliti zeSirimeye, qiniseka ukuba inqaku elinolwazi: Isihloko se "Slingee Slitter" 50 Imibuzo ebuzwa rhoqo

Cinga ngokuzinzisa isampulu yakho ukuthintela ukonakaliswa kohlalutyo.

Uhlengahlengiso:Ukulungelelanisa i-PH kunokunceda ukuzinzisa abahlalutyi. Umzekelo, i-acidic compovents inokufuna ukwahlula-hlulwa ukuthintela ukonakaliswa.

Izongezo:Ukongeza ii-Stabilibers okanye izinto zokugcina izinto kunganceda ukugcina ingqibelelo yesampulu ngexesha lokugcina kunye nohlalutyo.

Kubalulekile ukukhetha isinyithi esichanekileyo sokunyibilikisa isampulu yakho kunye nokuqinisekisa ukuhambelana kwenkqubo yakho ye-HPPC. Khawuqwalasele oku kulandelayo:

I-Solvent Poliney:Ukufezekisa ukuchithwa okugqityiweyo, isinyibilikisi kufuneka sihambelane ne-polady yohlalutyo.

Isigaba senqanaba leSoftware: Sebenzisa isoyile esihambelana nenqanaba leselula ukunciphisa izifundo ezikhoyo.

7. Ukulungiswa kwemigangatho

Ukulungiswa kwemigangatho yokulinganisa kubalulekile ukuqalisa uhlalutyo kwisampulu. Oku kubandakanya:

Ukulungiswa kwejikari yokulinganisa:Lungiselela uthotho lwezinto ezinomboniso wohlalutyo ukuseka ijika lokulinganisa.

Sebenzisa isolvent efanayo:Ukugcina ukungangqinelani, soloko ulungisa imilinganiselo yakho usebenzisa isoziso kunye neemeko ezithile njengesampulu yakho.

Landela iinkqubo zokusebenza ezisemgangathweni (i-SOPs):Ukuseka kwaye ulandele ngqo i-SOPs yelungiselelo lesampulu ukuqinisekisa ukungqinelana nokuzala kwakhona.

Sebenzisa i-regegents yomgangatho ophezulu kunye nezinyibilikisiweyo:Soloko usebenzisa izinyibilikisi ze-HPLC kunye ne-Regement yokuCebisa eziPhezulu kakhulu ukunciphisa ungcoliseko.

Gcina indawo yakho yokusebenza icocekile:Gcina indawo yokulungiselela isampulu icocekile kwaye ilungelelanisiwe ukunciphisa umngcipheko wongcoliseko.

XHO96 Qwalasela yonke into:Gcina iirekhodi ezineenkcukacha zeenkqubo zokulungiselela iisampulu, kubandakanya nakuphi na ukuphambuka kwiiprotokholi ezisemgangathweni.

Iindlela eziqinisekisiweyo:Ukuqinisekisa iindlela zokulungiselela isampulu rhoqo ukuqinisekisa ukuba babonelela ngeziphumo ezithembakeleyo nezibuyisiweyo.

Ubume obuphoselweyo:Ukuba iincopho zibanzi okanye zisecaleni, oku kunokubonisa ukuchithwa okungaphelelanga okanye ubukho beenkcukacha. Qinisekisa ukuba isampulu yakho ixutywe ngokupheleleyo kwaye ihlulwe.

I-Phost Peask:Iincopho ze-Ghost zinokubangelwa kukungcola kwisinyibilikisi okanye isampulu, zisebenzise izinyibilikisi eziphezulu kwaye ziqinisekise ukuba zonke iiglasi kunye nezixhobo zicocekile.

Imvakalelo ephantsi:Ukuba imvakalelo iphantsi, cinga ngokukhethekileyo indlela yakho yokuqina okanye ukwandisa ukugxininiswa kohlalutyo lwakho.

Ukuvala ikholamu:Ukuvalwa rhoqo kunokubonisa ukuba isampulu yakho inamachaphaza amaninzi okanye angabathwanga ngokufanelekileyo. Phinda uvavanye inkqubo yakho yokufihlakeleyo kwaye uqwalasele ukufihlakala.

Iziphumo ezingahambelaniyo:Ukwahluka kweziphumo kunokufumana isiqu sesampulu engahambelaniyo. Yenza iMigangatho Zonke iinkqubo kwaye uqiniseke ukuba abasebenzi ababandakanyekayo kumalungiselelo esampulu baqeqeshelwe ngokufanelekileyo.

Njengoko imfuno yohlalutyo inyuka, iilebhu zangasamkela ngakumbi iindlela zokulungiselela iisampulu zokuphucula ukusebenza nokuchaneka:

I-Spe yindlela esetyenzisiweyo esetyenzisiweyo evumela ukuqinisekiswa kohlalutyo kuhlahlo-mahlulo lwematriki entsokothileyo. Kubandakanya ukudlulisela isampulu yereyitha nge-adsorbyblerblerblerblerblerblent egcina uhlalutyo ekujolise kulo ngelixa uvumela ukungcola ukuba kudlula.

I-LE yindlela eyahlulahlulo ngokusekwe kulwelo lwazo kwiziselo ezibini eziguqukayo. Le ndlela isebenza ngokwahlulahlulahlulahlulahlulwa kwezinto ezahlukeneyo zendalo kwaye zihlala zisetyenziswa kuhlalutyo lwendalo kunye nohlalutyo lwamayeza.

Imibuzo yindlela ethandwayo yokulungiselela ukutya kunye neesampulu zokusingqongileyo ezidibanisa amanyathelo okukhutshwa kunye nokucoca kwinkqubo enye, eyenza isebenze kwaye ineendleko.

Ukukhutshelwa kwemveliso kunye nokuzala kunokuphuculwe kakhulu ngeendlela zesampulu ezizenzekelayo, ezinokwenza imisebenzi enjengemisebenzi, ukufihlakala, kwaye singeneleli ngongenelelo lomntu, ngaloo ndlela kuncitshiswa umngcipheko weempazamo.


Ufuna ukwazi ngakumbi malunga nokulungiselela isampulu ye-HPLC, nceda ujonge eli nqaku: I-HLLC yesampulu yokulungiselela ukulungiselela iziphumo ezilungileyo

Ukulungiswa kwesampulu ye-HPLC linyathelo elibalulekileyo elinokuchaphazela kakhulu ukuchaneka kunye nokuthembeka kweziphumo zohlalutyo. Ngeendlela ezilungileyo zokusebenzisa, ukusebenzisa iindlela ezifanelekileyo, kunye nokulungisa iingxaki eziqhelekileyo, iilebhu zinokwandisa inkqubo yenkqubo yesampulu ngokuthatha ubuchwephesha obuqhubela phambili kunye neetekhnoloji njengoko iimfuno zohlalutyo zivela. I-Automation iya kuphucula ngakumbi ukusebenza kakuhle nokusebenza kwempumelelo kohlalutyo lwe-HPPC. Ukulungiswa kwesampulu efanelekileyo ayiphuli nje umgangatho weziphumo, kodwa kukhusela inkqubo ye-HPLC, ukuqinisekisa ukuthembeka kwexesha elide kunye nokusebenza kwexesha.