I-Chromatography-Mass Spectrometry (LC-MS) yindlela enamandla yohlalutyo edibanisa amandla okwahlulahlula i-chromatography ye-spectrometry ye-spectrometry ye-spectrometry. Indlela isetyenziswa kuluhlu olubanzi lwamabala, kubandakanya amayeza, uhlalutyo lokusingqongileyo, kunye nendlela yokuxilongwa kwezonyango. Inxalenye ephambili yeNkqubo ye-LC-MS yi-autosampler, edlala indima ebalulekileyo ekusebenzeni nasekuthembekeni kohlalutyo. Kule bhlog, siya kuphonononga ukubaluleka kweI-autosampler vialsKuhlalutyo lwe-LC-DS, uyilo lwabo, iintlobo, kunye neendlela ezilungileyo zokufezekisa intsebenzo efanelekileyo.

Ukuqonda i-autosampler vials

I-Vials ye-Autosampler zizikhongozeli ezincinci ezisetyenziselwa ukubamba iisampulu zetroli ezingeniswe kwiNkqubo ye-LC-ms yohlalutyo. Ezi vils zenzelwe ukuba zingene kwi-autosampler, ethi ikhuphe ngokuzenzekelayo ivolumu efanelekileyo yesampulu kwinkqubo yeChromatography. Ukukhetha i-vial kunokuchaphazela kakhulu ukusebenza kohlalutyo lwe-LC-ms, echaphazela izinto ezinje ngentembeko yesampulu, ungcoliseko, kunye nokuzala.

Vula iimfihlo ze-veromatography ye-veromatographys efanelekileyo yokuhlalutya ngokuchanekileyo nangokuthembekileyo kumanyathelo ama-6 nje alula. Funda ukuze ufumane inkosi ubuchule! Amanyathelo ama-6 okulungiselela i-vialsgraphy ye-vials yohlalutyo

Iimpawu eziphambili ze-vials ze-autosampler

Izikhongozelo zesandla:I-autosampler vials ibambe iisampulu ngokukhuselekileyo ukuthintela ungcoliseko kunye nokuphuma. Uyilo lwe-Vic luqinisekisa ukuba isampulu ihlala izinzile ngaphambi kokuba ifakwe kwinaliti kwi-LC-NS System.

Ukuhambelana:I-vial kufuneka ihambelane nenkqubo ye-autosampler kunye ne-LC-NS. Oku kubandakanya ukuqwalaselwa njengobukhulu be-vial, ubukhulu bentamo, kunye nokuhambelana kwezinto ezibonakalayo kunye nabahlaziyi.

Impendulo yesampulu:Imixholo ye-vial inokuchaphazela uzinzo lwesampulu. Umzekelo, ezinye iiplastiki zinokuphelisa iqulathelwa kwisampulu okanye zihlalutye, zikhokelela kwiziphumo ezichanekileyo. I-vials yeglasi ihlala ikhethwa ngenxa yobuso babo.

Ukusetyenziswa ngokulula:I-vial kufuneka ibe lula ukuphatha kwaye ilayishe kwi-autosampler. Iimpawu ezinje ngophuhliso lwevolumu kunye neelebheli ezicacileyo zelebheli ziyayixhasa i-canisance.

Iindidi ze-vials ze-autosampler

Zininzi iindidi ze-vials ze-autsampler zokukhetha kuzo, yenzelwe iZicelo ezithile kunye neemfuno:

1. Ii-vials eziqhelekileyo

I-Vials esemgangathweni zezona ndlela zisetyenziswa kakhulu kuhlalutyo lwe-LC-ms. Ngokwenziwe ngokwesiqhelo ngeglasi okanye ngeplastiki esemgangathweni ephezulu kwaye baza kwiintlobo ngeentlobo, ezinje nge-1 ml, 2 ml, kunye ne-4 ml. Ezi vils zilungele uluhlu olubanzi lwezicelo kwaye ziyahambelana ne-autosamplers.

I-2. I-screw cap vials

Isikhafu se-capYiba nentamo ekhutshelweyo etywine ngokukhuselekileyo ngentloko. Olu luyilo lunceda ukukhusela ukuvela kunye nokungcolisa. I-screw cap i-vials ilungele iisampulu eziguqukayo okanye iisampulu ezifuna ukugcinwa kwexesha elide.

I-3. Crimpp eqongeni

I-crimpp ephezulu ye-vialszitywinwe nge-alminium crimp ye-caps ebonelela ngetywina lomoya, ngakumbi iisampulu ezibuthathaka zomoya. Ezi vils zihlala zisetyenziselwa iisampulu ezifuna uzinzo oluphezulu kunye nokukhusela ekuthambekeni kwento.

I-4. I-Vials ekhethekileyo

I-vials ekhethekileyo yenzelwe izicelo ezithile, ezinjengezo zifuna ukulungiselela isampulu okanye ziqukethe uhlalutshelo. Umzekelo, i-vials ezineefilitha ezihlanganisiweyo zinokunceda ukususa amasuntswana ngaphambi kokuba inaliti, ngelixa i-vials enemitya ethile inokunciphisa i-adsorvo yohlalutyo.

Ufuna ukwazi ukuba ukhetha njani i-crmial vs vs vial vs. screw cap vial ?, Jonga eli nqaku: I-crmp vial vs i-vial vial vs.crew cap vial, njani?

Ukubaluleka kokukhetha i-Vial kwi-LC-ms Recolly

Ukukhetha i-vial ye-autonampler efanelekileyo kubalulekile ukufumana iziphumo ezichanekileyo nezithembekileyo ze-LC-ms. Nazi ezinye izinto eziphambili ekufuneka ziqwalaselwe xa ukhetha i-vial:

1. Ukuhambelana kwamachili

Izinto ze-vial kufuneka zihambelane nesinyibilikisi kunye nohlalutyiweyo ukuba zihlalutywe. I-vials yeglasi ngokubanzi kwaye ilungele uninzi lwezicelo, ngelixa ezinye iiplastiki zinokusebenzisana nezicoci okanye zihlalutye, zibangele ungcoliseko.

I-2. Isampulu yesampulu

I-vial kufuneka ibambe ivolumu yesampulu efunekayo yohlalutyo. I-vials eziqhelekileyo zihlala zibamba i-1 ml ukuya kwi-5 ml, kodwa ezinye izicelo zinokufuna umthamo omkhulu. Ukuqinisekisa ukuba ubungakanani be-vial kufanelekile kwiimfuno zevolumu ye-autosampler ibalulekile.

I-3. Ukuvalwa kwe-vial

Indlela yokuvala ye-vial inokuchaphazela ukuthembeka kwesampulu. Isikhafu se-cap kunye ne-crimps ye-crals ibonelela ngeendlela ezahlukeneyo zokutywina, kwaye ukhetho luxhomekeke ekuzinzisweni kunye nokutywina kweepropathi zesampulu. Ukutywina ngokufanelekileyo i-vial ithintela i-vial kunye nongcoliseko ngexesha lokugcina nokuphatha.

4. Ukucoceka kwe-vial

I-autosampler vialsKufuneka icoceke kwaye ingenazizathu ezingcolisekileyo ezinokuthi ziphazamisane nohlalutyo. Kucetyiswa ukuba usebenzise i-vials ecocekile kwangaphambili kwaye iqinisekiswe izicelo ze-LC-ms ukuqinisekisa ukuthembeka kwesampulu.

I-5. Iindleko kunye nokufumaneka

Ngelixa umgangatho ubalulekile, iindleko kunye nokufumaneka kwakhona kudlala indima ekukhetheni i-vial. Iilebhu kufuneka zilungelelanise imfuno yee-vials eziphezulu eziqwalaselwa zohlahlo-lwabiwo mali kwaye ziqinisekise ukuba ii-vials ezikhethiweyo zifumaneka ngokulula ukuze zisetyenziswe rhoqo.

Iindlela ezilungileyo zokusebenzisa i-autosampler vials kwi-LC-NS

Ukuqinisekisa ukusebenza kakuhle kuhlalutyo lwe-LC-MS, cinga ngezi zinto zilandelayo zokusebenzisa i-autosammar vials:

I-1. Ukuphatha ngokufanelekileyo kunye nokugcina

Phatha ii-vials ngononophelo ukunqanda ungcoliseko. Nxiba iigloves xa uphatha i-vials kunye neesampulu ukunciphisa umngcipheko wokwazisa ungcoliseko. Gcina i-vials kwindawo ecocekileyo, eyomileyo ukuthintela ukonakaliswa kwesampulu.

2. Sebenzisa uhlobo olufanelekileyo lwe-Vial

Khetha uhlobo olufanelekileyo lwe-vial ngokusekwe kwiimfuno ezithile zohlalutyo. Kwiisampulu eziguquguqukayo, cinga nge-cap etywiniweyo okanye i-crimps ephezulu ye-vials. Kwiisampulu ezifuna ukuzinza, sebenzisa i-vials ngeefilitha ezihlanganisiweyo.

I-3. Ukulungiswa rhoqo kwi-autsampler

Gcina kwaye ulungelelanise i-autosampler rhoqo ukuqinisekisa inaliti echanekileyo nengaguqukiyo. Izinto ezicocekileyo ze-autosampler, kubandakanya nenaliti kunye nezibuko le-inaction, ukuthintela ungcoliseko olunqamlezileyo phakathi kweesampulu.

I-4. Yenza ivolumu yesampulu

Qinisekisa ukuba ivolumu yesampula ilungele amandla e-autosampler. Ukugqwesa okanye ukuqhubela phambili i-vial kunokukhokelela kwiimposiso zenaliti kwaye unciphise umgangatho wohlalutyo.

5. Inkqubo yokulungiselela isampulu

Gcina iirekhodi ezineenkcukacha zeenkqubo zokulungiselela isampulu, kubandakanya ukhetho lwe-vial, ivolumu yesampulu, kunye naluphi na utshintsho olwenziwe kwiprotocol enobume. Le rekhodi ibalulekile ekuveliseni kwakhona kunye nokuthotyelwa kwemigangatho yolawulo.

I-Wan Ukufumana ulwazi olupheleleyo malunga nendlela yokucoca i-vialsgraphy yesampulu ye-chromatography, nceda ujonge eli nqaku: Iyasebenza kakuhle! Iindlela ezi-5 zokucoca iimodeli ze-chromatographys

Ukuqukumbela

I-autosampler vials idlala indima ebalulekileyo kwimpumelelo yohlalutyo lwe-LC-ms. Uyilo lwabo, ukuhambelana kwazo, kunye nokutywina kweendlela ezinefuthe ngqo kwintembeko yesampulu, umngcipheko wokungcola, kunye nokusebenza ngokubanzi. Ngokuqonda ukubaluleka kokukhetha i-vial kunye nokubambelela kwihambo elungileyo, iilebhu inokuphucula ukuthembeka kunye nokuchaneka kohlalutyo lwe-LC-ms yabo. Njengoko imfuno yokufumana iindlela ezinokuhlalutye ziyakhula, indima ye-vials ye-andespler iya kuhlala ibalulekile ekuqinisekiseni umgangatho wezicelo kwizicelo ezahlukeneyo zophando kushishino.

Ngaphambili:

Yonke into oyifunayo ukwazi malunga nokulungiselela isampulu ye-HPLC

Okulandelayo:

Nguwuphi umahluko phakathi kokuhlalutya i-HPLC?

Iimpawu: I-H Plcanalysis I-lab-vials I-Automomvvials I-HIPLCEAMAMAMAMAMAMAMAME I-HLLC I-autosampler-vials I-2ml-i-autosampler-vials I-4ml-screw-ephezulu-i-autosampler-vial

Umbuzo