I-Propace Promatography (HS-GC) yindlela enamandla yohlalutyo ekuhlalutyeni iziqu ezihlanganisiweyo kwiintlobo ezahlukeneyo zeMatriki, kubandakanya ulwelo kunye nezinyibilikisi. Indlela ikhupha ngokufanelekileyo indlela uhlalutyo oluguqukayo ukusuka kwimatriki entsokothileyo ngaphandle kokufaka isampulu yonke ngqo kwi-chromatograph yegesi (GC). Endaweni yoko, i-HS-GC ihlalutya isigaba segesi ngaphezulu kwesampulu, eyaziwa ngokuba yiPOECE. Le bhlog iyakugubungela yonke into ofuna ukuyazi malunga ne-ERPACE GC GC yesampulu yesampulu, kubandakanya imigaqo, ubuchule, izibonelelo, kunye neendlela ezilungileyo.

Ufuna ukwazi ngokupheleleyo malunga ne-vial ye-vial, nceda ujonge eli nqaku: Isikhokelo esibanzi kwi-vials ye-EPECE: Iimpawu, ukhetho, ixabiso, kunye nokusetyenziswa

Ukuqonda iSampulu yeentloko

ISampungo ye-PROPECE isekwe kumgaqo wokuba imiqathango eguqukayo kwisampulu inokwahlula kwinqanaba legesi ngaphezulu kwesampulu xa ikhutshiwe okanye ilungelelaniswe. Indlela iluncedo ngokukhethekileyo ekuhlalutyeni iziqu ezingcolisekileyo ze-orting IiNdlela eziPhambili zinciphisa intshayelelo yamacandelo angenamathungo kunye nongcoliseko kwinkqubo ye-GC, okukhokelela kwi-chromatoograms ecocekileyo kunye neziphumo ezinokuthenjwa.

Iikhonsepthi eziphambili zeSampulu ye-ERMECE

I-Equilibrium:Xa isampulu ibekwe ngaphakathi i-vial evaliweyo kwaye ifudumekile, imiba eguqukayo iya kutyeshelwa ukusuka kulwelo okanye isigaba esiqinileyo kwisigaba segesi kude kufikelwe ukulingana. Uxinzelelo lohlalutyo kwindawo entloko luxhomekeke ekuzinzisweni kwayo kunye nobushushu besampulu.

Ulwahlulelo lwe-coandeal (k):Isahlulelo sokusebenza sinento ephambili kwi-Sampace ye-EREPE. Ichaza ukuhanjiswa kweendlela eziguquguqukayo phakathi kwezigaba ezingamanzi kunye negesi. Imithombo enamagqabantshintshi ephantsi ye-K bathabathela ngokulula kwisigaba segesi kwaye ke bafunyanwa ngokulula.

I-Static vs space enkulu:I-Sampace Patling inokwenziwa kusetyenziswa iindlela ze-Static okanye ezinamandla. I-Static Stace iquka ukuvumela isampulu yokufikelela kwi-equilibrium kwi-vili ye-Vial, ngelixa i-vilpace eguqukayo ibandakanya ukucoca isampulu yegesi yokutshayela igesi yegesi.

I-Propace Promatography yesampulu yesampulu

Ukulungiselela kwesampulu efanelekileyo kubalulekile ekufumaneni iziphumo ezichanekileyo nezibuyisiweyo kwi-PARPECE SPREATOTOGY La manyathelo alandelayo achaza iingcinga ezisisiseko zokulungiselela iisampulu zohlalutyo lweentloko.

1. Khetha isampulu yasekunene vial

Ukukhetha ilungeloisampulu ye-vialibalulekile kwi-Sampling ye-PROPE COPECE. Ubungakanani be-vial eqhelekileyo bubandakanya i-6ml, i-10ml, kunye ne-20ml, kunyeI-20ml vialsukuba sesona sisetyenziswa kakhulu. Izinto eziphambili ekufuneka ziqwalaselwe xa ukhetha i-vials ibandakanya:

Izixhobo:Ii-vials zihlala zenziwe ngeglasi okanye ngeplastiki.I-vials yeglasizilungele iisampulu eziguquguqukayo ngenxa yokungena kwabo kwaye zisezantsi zezinto ezingcolisayo.

Indlela yokutywinwa:I-Vials inokutywina nge-crump okanye i-screw caps. I-crmp vialsNika itywina le-airlight, ekubaluleke kakhulu ukugcina ingqibelelo yendawo entloko.

Umgangatho we-Septum:I-Septa yayisetyenziselwa ukutywina ii-vials zinokuzisa ungcoliseko ukuba ngaba balungelelene. Jonga iSepta yenzelwe ngokukodwa izicelo zentloko, njengoko zinokwenzeka ukuba ziqengqele kwindawo entloko.

2. Isampulu yesampulu kunye ne-dilution

Umthamo wesampulu kwi-vial ibalulekile ekufezekiseni inkqubela phambili yentloko efanelekileyo. Ngokubanzi, ivolumu yesampulu kufuneka ijikeleze i-1 \ / 3 ukuya kwi-1 \ / 2 yomthamo opheleleyo we-vial ukubonelela ngesantya esiphezulu seSigaba seGalo.

I-dilution:Ukuba ukugxininiswa kwesampula kuphakame kakhulu, kungakhokelela ekunyamezeleni okugcweleyo, okukhokelela kubungakanani obuchanileyo. Ukutyibilika isampulu kunye nesinyibilikisi esifanelekileyo kunokunceda ekufezekiseni ukugxininiswa okufunekayo kubahlalutyo abaguqukayo.

Ngaba ufuna ukwazi ngakumbi malunga nokuba kutheni i-villa ye-Promatoces esetyenziswe kwiChromatography ?, Nceda ujonge le arcece: Kutheni le nto i-villa ye-ERPE isetyenziswa kwiChromatography? Ii-Angles ezili-12

3. Ulawulo lobushushu

Iqondo lokushisa lidlala indima ebalulekileyo kwisampulu ye-5 njengoko ichaphazela ukungazibazwa kohlalutyo kunye nokwahlula kwabo kwinqanaba legesi.

Iqondo lokushisa:I-vials yesampulu kufuneka ifuduselwe kubushushu obulawulwayo ukukhuthaza ukukhutshwa kweendlela zokuzonwabisa kwindawo ephambili. Iqondo lobushushu elifanelekileyo lixhomekeke kuhlalutyi ethile ukuba zihlaziywe kwaye kufuneka zimiselwe ngexesha lokuphuhliswa kwendlela.

Ixesha lokulinganisa:Vumela ixesha elaneleyo lesampulu yokufikelela kwi-equilibrium. Oku kunokwahluka ngokuxhomekeke kwi-matrix yesampulu kunye nokuzinza kwamachiza. Amaxesha okuqhelekileyo ahlukeneyo ukusuka kwimizuzu engama-30 ukuya kwiiyure ezininzi.

4. Nciphisa ungcoliseko

Ungcoliseko luchaphazela kakhulu ukuchaneka kwe-PAGAG i-Chromatography. Ukunciphisa umngcipheko wongcoliseko, yenza oku kulandelayo:

Sebenzisa i-vials ecocekileyo:Soloko usebenzisa i-vials ecocekileyo yokuphepha ukungeniswa kwamanyala ngexesha lokupakishwa okanye ukuphatha.

Iindlela ezingenanto:I-Rung Fight Kids ukuze ichonge imithombo yongcoliseko. Oku kubandakanya ukuhlalutya isampulu engenanto kusetyenziswa iindlela zokulungiselela kunye neenkqubo zohlalutyo ukuqinisekisa ukuba akukho zincopho zingafunekiyo zivela kwiChromatom.

Iimeko ezilawulwa ngemo engqongileyo:Yenza amalungiselelo esampulu kwindawo ecocekileyo yokunciphisa ukuvezwa kwi-aminants engcolileyo.

5. Khetha ubuchule basekunene

Njengoko bekutshiwo ngaphambili, isampulu yentloko inokuba yindawo ye-static okanye inamandla. Ukhetho lwendlela luxhomekeke kwisicelo esithile kunye nohlobo lwesampulu.

I-Static Espace:Le ndlela ilungele ukusetyenziswa kakhulu kwaye isetyenziswa ngokubanzi ukuhlalutya imibandela ye-witlatile kwiziselo kunye nezinyibilikisi. Ivumela abahlalutyi ukuba bahlulele ngokwendalo kwindawo enkulu ngaphandle kokuqaliswa kwegesi eyongezelelweyo.

Iphepha elinamandla:Obu buchule bufanele bube ngcono kwiisampulu ezifuna ukuphefumla okuqhubekayo ukufaka imizobo ye-withise. Ihlala isetyenziswa kwizicelo ezifana nokuvavanywa kokusingqongileyo kunye nohlalutyo lokutya apho iisampulu zinokuqulatha ukugxininisana kohlalutyo.

IINGXAKI ZOKUXHASA ISAMPELULEKILEYO

IChroomatoograms:Ngokuhlalutya kuphela isigaba se-vapor kuphela, isampulu ye-ERPE incipha ukuvelisa izinto ezingezizo ezingezizo ezingezizo kunye nezingcoliseko, ezikhokelela kwi-chromatoms ye-chromatograms kunye nokuphuculwa kokusebenza komsebenzi.

Inciphise isampulu yamalungiselelo emodeli:ISampungo ye-PROPECE SPPEL YAYENZA INKQUBO YOKULUNGISELELA ISAMBALA, Njengoko isusa isidingo sonyulo oluntsonkothileyo okanye iinkqubo zokucoca.

Ukonyuka kwemvakalelo:Ukugxininiswa kohlalutyi-hlulo oluguqukayo epaniyo kunokukhokelela ekuphuculeni ubuntununtunu, kokwenza kube lula ukubukela imigangatho yenqanaba le-trace.

Ukutshintsha:Ukuqhutywa kwesampulu ye-PRAPE kungasetyenziswa kuluhlu olubanzi lweendidi zesampulu, kubandakanya ulwelo, iinyibiliki, kunye neendlela ezinzima, zenza ukuba zibe yindlela yokutshintsha izicelo ezahlukeneyo.

Ufuna ukwazi ngakumbi malunga ne-vials ye-aijiren vials kunye ne-caps, nceda ujonge le arcece: Luthini umlinganiso we-aijiren vials kunye ne-caps?

Ukuqukumbela

I-Propace ye-Promatographyyindlela exabisekileyo yohlalutya kuhlalutyo lweengcongconi zeempuku kwiiMatriki ezahlukeneyo zeMatriki. Ukulungiswa kwesampulu efanelekileyo kubalulekile ekufezekiseni iziphumo ezichanekileyo nezibuyisiweyo kwi-RPA Uhlalutyo lwe-GC. Ngokuqonda imigaqo-siseko yesampulu yentloko kunye nokulandela iinkqubo ezifanelekileyo zesampulu, iilebhu zinokuphucula ukusebenza kunye nokuthembakala kohlalutyo lwazo. Njengoko imfuno yeendlela zokuhlalutya ezisemgangathweni ezisemgangathweni ziyakhula, isampulu ye-ERPE GC iya kuhlala sisixhobo esibalulekileyo kubaphandi kunye nabahlalutyi kuzo zonke iinkqubo ezininzi.

Ngaphambili:

I-HPPC vs. LCMS: Nguwuphi umntu ofanele ukhethe? Amanqaku ama-5

Okulandelayo:

Ungasisebenzisa njani ngokufanelekileyo iityhubhu zovavanyo lwe-COD zovavanyo lomgangatho wamanzi?