I-chromatography ephezulu ye-chromatography (i-HPPC) kunye ne-chromatography ye-chromatometry-ubunzima beendlela ezimbini zohlalutyo ezisetyenziswa kwiindawo ezahlukeneyo zohlalutyo ezisetyenziswa kwiintlobo ngeentlobo zamachiza, iiSayensi zeNdalo kunye neNzululwazi yoBomi. Ngelixa zombini iindlela zibandakanya ukwahlukana kunye nokuhlaziya imizobo, imigaqo-siseko, imisebenzi, imisebenzi kunye nezicelo zahluke kakhulu. Eli thuba le-Blog liya kuthelekisa i-HLLC kunye ne-LCLMS, igxile kumahluko wabo ophambili kunye nezibonelelo zendlela nganye.

Imigaqo yokusebenza

Hlc

I-HPLC yindlela ye-chromatographic eyahlulahlulahlulahlulo ngokusekwe kunxibelelwano lwabo nezigaba zesikhululo kunye nesoftware. Kwi-HPLC, iSigaba seNgingqi ye-Liquil sikhutshwe ngekholam esizaliswe sisigaba sesikhululo, esidla ngokudityaniswa kwamasuntswana amancinci esilica okanye i-polymers. Xa umxube wesampulu waziswa kwinkqubo, amacandelo ayahlulwe ngokusekelwe kubumba bawo kwinqanaba lesikhululo. Imigqaliselo enxibelelana ngakumbi kunye neSigaba seSigaba seSitimela ngokuthe ngcembe, ngelixa imiqathango edibeneyo inxibelelana kakhulu Imithombo edibeneyo ichongiwe isebenzisa izixhobo ezahlukeneyo, ezinjenge-UV-Vis, i-fluorescence, okanye i-Recint Recingens.

Ufuna ukwazi ngakumbi malunga nokusetyenziswa kwe-vialsgraphy ye-vials, nceda ujonge eli nqaku:I-15 izicelo ze-vialsgraphys kwimimandla eyahlukeneyo

I-LCMS

I-LCMS idibanisa amandla okwahlulahlula i-HPLC kunye nezakhono zohlalutya oluninzi kwi-spectrimetry. Kwi-LCMS, iisampulu zahlukane kuqala usebenzisa i-HPLC, njengokuba ne-HPPC eqhelekileyo. Nangona kunjalo, endaweni yokufunyanwa ngumzingeli oqhelekileyo, amakhonkco appetweyo azaziswa kwi-spectrometer enkulu. I-Mass Sercrometer ion i-computers kwaye isebenzise umyinge wawo ohlawulelwayo, ubonelela ngolwazi oluneenkcukacha malunga nobunzima bemolekyuli kunye nolwakhiwo. Indibaniselwano yokwahlukana kunye nobunzima obuninzi bubonelela ngovakalelo olukhulu kunye nobungqina be-hplc kuphela.

Indlela yokwahlula

I-HPPC:I-HPPC isebenzisa uxinzelelo oluphezulu lokutyhala iSigaba seNgingqi ye-Liqual kwikholamu esenziwe ngepakeji equkethe isigaba sesikhululo. Imigqaliselo zahlulwe ngokusekwe kunxibelelwano lwawo lohlukeneyo kunye nenqanaba lesikhululo, elikhokelela ekuzinziseni nokuzibandakanya ngokufanelekileyo.

I-LCMS:I-LCMS idibanisa amandla omzimba we-HPLC kunye ne-Serctrametry ye-Spectrometry ye-spectrometry (ms). Icandelo le-chromatography ye-chromatography lahlulahlulahlula, ngelixa i-spectrometer enkulu ilinganisa umyinge we-5

Imvakalelo kunye nokukhetha

I-HPPC:I-HPPC inemvakalelo elungileyo kunye nokukhetha, ngakumbi xa idityaniswa nabachongi abaphambili anjenge-UV-Vioresce, i-fluorescence, okanye diode oocenga. Nangona kunjalo, i-HPPC yedwa ayinakubonelela ngobuqili obaneleyo kwiisampulu ezintsonkothileyo.

I-LCMS:I-LCMS inemvakalelo ebalaseleyo kunye nokukhetha ngokuthelekiswa ne-HPPC. Abathengisi bemidlalo yobunzima banokuchonga ngokuchanekileyo kwaye basebenzise imidibaniso esekwe kwimilinganiselo yabo eyahlukileyo yokuhlawula, nkqu nakwimatriki entsokothileyo. Oku kwenza ukuba i-LCSMS iluncedo ekuhlalutyeni umkhondo wokulandela umkhondo kunye nokuqinisekisa ukuba izinto ezingaziwayo.

Ukulungiswa kwesampulu

I-HPPC:Ukulungiswa kwesampulu ye-HPPC kubandakanya ukubandakanya i-dilution, ukufihlakala, okanye iindlela zokukhuthaza okulula. Iisampuli azidingi ukuba zikhutshiwe, ke i-HPPC ilungele uluhlu olubanzi lweendawo ezidityanisiweyo, kubandakanya izinto ezingeyonyani.

I-LCMS:Ukulungiselela isampulu ye-LCMS kuyafana ne-HPLC, kodwa kuya kufuneka i-HPLC, kodwa kuya kufuneka i-HLLC, kodwa kuya kufuneka inyathelo ezongezelelweyo ukuqinisekisa ukuhambelana ne-spectrometer enkulu. Umzekelo, ii-buffers eziguquguqukayo okanye ezongeziweyo zinokufuneka ukongeza i-ions kunye nokukhusela uxinzelelo lwe-ion.

Izicelo

I-HPPC:I-HPLC isetyenziselwa ngokubanzi uhlalutyo lwamayeza, ukutya kunye neesampulu zokusingqongileyo. Kuyimpumelelo ngakumbi ukwahlulahlula kunye nokwahlulahlulahlula imixube emintrandi, enjengokungcola kwamachiza, izongezelelo zokutya, kunye nentsalela yamayeza.

I-LCMS:I-LCMS inoluhlu olubanzi lwezicelo, kubandakanya:

Uhlalutyo lwamachiza: Isetyenziswe ekufumaneni iziyobisi, uphuhliso kunye nolawulo oluphambili.

Iproteomics kunye neMetabomics: isetyenziselwa ukuchonga nokuthintela iiprotherini kunye neMetabolites kwiisampulu zebhayoloji.

Uhlalutyo lokusingqongileyo: Isetyenziselwa ukufumana umkhondo emanzini, umhlaba kunye neesampulu zomoya.

Ndinomdla kwindima ye-vials eqinisekisiweyo ye-HPLC ekuqinisekiseni uhlalutsho oluchanekileyo? Funda eli nqaku: Ukubaluleka kwe-vills eqinisekisiweyo ye-HPPL yohlalutyo lweSampulu oluchanekileyo

Ukuqukumbela

I-HLLC kunye nee-LCMs zimbini iindlela zokuhlalutya kunye namandla abo kunye nokusikelwa umda. Ukukhetha phakathi kwezi zimbini kuxhomekeke kwiimfuno ezithile zohlalutyo, ezinjengokuziva, ukukhetha, isampulu, kunye nezixhobo ezikhoyo.

I-HPPC lukhetho olulungileyo kuhlalutyo lwendlela eyaziwayo kwiimatriki ezilula, apho iindleko kunye nokusetyenziswa lula zibalulekile. I-LCMS, kwelinye icala, yindlela ethandwayo yokuhlalutya iisampulu ezintsonkothileyo, echonga izixhobo ezingaziwayo, kwaye iphumelele ukufunyanwa kwenqanaba le-Ultra.

Njengoko itekhnoloji iyaqhubeka ukuqhubela phambili, ukudityaniswa kwe-HPLC kunye nee-LCMS kuya kudlala indima ebalulekileyo ekufezekiseni iimfuno zokuvela ze-chemistry yohlalutyo kunye nokuqinisekisa ukuba iziphumo ezisemgangathweni eziphezulu.

Ngaphambili:

Isikhokelo sokugqibela kwi-0.45 icebo leefilitha ze-syringer yakho

Okulandelayo:

Yonke into ofuna ukuyazi malunga ne-ERESCE GC GC