Samfura samfurin lcms shiri: Tabbatar da daidaitaccen bayanai tare da caja
A cikin binciken LC-MS, kowane mataki na samfurin samfurin yana da mahimmanci, musamman zaɓi da amfani da ƙofofin vial da Septa. Wannan labarin da ke tsari da keɓen fasahohin da zai iya haɗawa, gami da tarko, hazo mai amfani, hakar ruwa mai ruwa, da derivatization softed, da lalata ruwa. Ya kuma bayyana yadda ake zaɓar Aijireen pre-silit ptfe tfe tfe tfe tfe tfe etfe etfe etfe etfe etfe etfe etfe etfe etfe etfe etfe etfe etfe etfe etfentos don tabbatar da amintaccen hatimi da kuma gurbata. Tare da tebur mai daidaituwa, masu karatu zasu iya fahimtar fa'idodin da kowane hula na kowane hula.
A cikin ruwa chromatography-taro spectrometry (LC-ms), shirye-shiryen samfar da ya dace yana da mahimmanci don samun cikakken sakamako mai aminci. Wannan tsari ya cire, ya tsarkaka, da mai da hankali kan bincike na manufa daga matsakaiciyar matrixes yayin kawar da ingancin bayanan da suka lalata ƙimar bayanai. Samfurin samfurin inganci Ta haka ne haɓaka hankali da kuma inginsa, wanda yake da mahimmanci ga filayen kamar bincike game da magunguna, ƙwayoyin cuta. Cikakkun bayanai kamar rufewa na vial na iya yin babban bambanci: leaks ko gurbata daga matalauta caps \ / septa zai sasanta samfurin kafin ya kai lc-ms.
Batu na Key: Yi amfani da ƙwararrun ƙwararrun Kayayyakin Aijireen da SPETA don hana gurbatawa samfurin da kuma tabbatar da amincin bayanai.
A jihar-of-art-Art-ms-ms-art kayan aiki a cikin dakin gwaje-gwaje, yana nuna mahimmancin shirye-shiryen samfurin meticous.
1. LCMs shirye-shiryen samfurin lcms
M
Nufi
Tanki
Yana cire ɓarke (0.2 μm matattara) don kare ginshiƙai da nozzles.
Ingancin Protein
Yana amfani da abubuwan da ke tattare da abubuwan da kwayoyin cuta (acetonol, methanol) don share sunadarai daga abubuwan sammai.
M lokaci na hakar (kera)
Ya mai da hankali kan nazarin kuma yana cire abubuwan kutse ta hanyar katako mai soron.
Fitar ruwa-ruwa (lle)
Ma'amalan Canza na Zama cikin Tsarin Kwayoyin; m amma iya emulsify.
Tallafi mai amfani da ruwa (seura)
Lle mai sarrafa kansa akan tallafin INERT; yana guje wa emulsion da kuma adana abubuwa.
Deseratization
Chise mix da aka tantance (E.G. MEMYLYA) don inganta ionization.
An yi amfani da tace sirinji na dakin gwaje-gwaje don tace samfurin cikin HPLC Vial, yana nuna shirye-shiryen shirye-shiryen Samfurin LC-MS.
2
A cikin LC-MS Workflows, ƙulli vial shine katangar ƙarshe kafin bincike. Septi mai inganci da ƙarfi suna tabbatar da satin da ke cikin iska, yana hana ruwa da kuma toshe gurbata muhalli.
PTFE \ / Silicone Selta: Chemony Inerer, yana rage karnuka.
Bonds iyakoki: Septum dindindin a haɗe zuwa hula, kawar da sako-sako da suttes.
Kyakkyawan dabara da aka dace kuma suma suna da mahimmanci: Temptly-karfin-karfafawa na iya tsayayya da Septa, karkashin-da-daukaka ke haifar da leaks. A amintacce, sutura ta inganta haihuwa kuma yana hana gurbata giciye.
Valomography Autosampler vial ya haɗa tare da PTFE \ / silicone Silicone Siltum da sikeli Cap, yana nuna tabbataccen hatimin VIAL don lc-ms samfuri.
Ya tsayar da cututtukan cututtukan ruwa; Haɗin ƙira yana hana septum canfing ko kumburi.
Deseratization
Bond PTFE \ / silicone silicone silicone silic
Yana ba da madaidaicin tsarin dogon lokaci game da ƙiren ruwa da danshi.
4. Adijireen Cap & Seepta samfurin kwatancen
Nau'in samfurin
Abu
Abubuwan da ke cikin key
Aikace-aikace
Pre-slit ptfe \ / silicone dunƙule
Ptfe \ / silicone silicone silsi + aluminum cap
Maimaitawa; inert; low cirewa
High-hanyar lc-ms, autosamplers
Daidaitaccen ptfe \ / silicone dunƙule
Ptfe \ / silicone silicone silsi + aluminum cap
Janar-manufa hatimi; yalwar da ya cancanta
Routine LC-MS Vials
Bonded Septum Coast
Al CO CO CoT + BOND Septum
Septum har abada an makale; Haske mai daidaituwa
Tsarin sarrafa kansa, tsarin ajiya na dogon lokaci
Snap Cap + roba
Septum septum + bakin karfe clomp
Maras tsada; Hadarin Coring
Kasafin kudi mai zurfi, masu bincike mara mahimmanci
Tebur: Aijireen Clicks Kwatanta.
Ƙarshe
Shiri na lc-ms samfurin shine tushen bayanan abin dogaro. Yin amfani da rufewar dama - kamar a cikin Aijire PTFE OTFE \ / silicone SePTa da kuma takunkumi-adana ƙayyadaddun mutuwar ta hanyar hana gurɓatawa da kuma zubar da gurbatawa. Matching da ƙulli ga hanyar ka ya tabbatar da cikakken sakamako. Don magalakalan Lab, magunguna na R & D, da kuma manyan biranen uku na ɓangare, suna zaɓen mahimmin karfin Adijia da Septa, hasashe, tarkace) da kuma goyan bayan m LC-MS Workflows.
Key Teaway: Ingancin vial da SePTA suna da mahimmanci don hana asarar samfurin kuma tabbatar da tabbacin daidaito. Mafita na Allutions Exutvate LC-MS aiwatar da aikin dakin gwaje-gwaje.
Wani mai binciken bincike na LC-ms Autosampler Vials tare da amintaccen iyakoki, misalai samfurin mai kulawa da kulawa.
