Ma ka loiloi LC-MS i keʻano o kēlā me kēia papa o ka hoʻomākaukauʻana i ka mākaukau,ʻoi aku ka koho a me ka hoʻohanaʻana i nā hale kūʻai kūʻai a me ka hoʻohana. Hoʻopiliʻia kēiaʻatikala i keʻano o nā hana hoʻomākaukau kūʻai, me ka filtertration, ka mea waiwai protein, i hoʻopiliʻia ai ka wai E wehewehe nō hoʻi i ke kohoʻana i ka majiren ma mua o Ailajen PTFE \ / Silicone Septa Me kahi papa hana huahana, hiki i nā mea heluhelu ke hoʻomaopopo maopopo i nā mea pono a me nā hana hana a me nā mea hōʻoia a me kaʻike.
Heluʻia4.8\ / 5 / ^ E pili ana594Nā Hoʻomaopopo Manaʻo
Ma ka wai chromatography-nui i ka spectrometry (lc-ms),ʻoi aku ka uku kūpono kūpono e loaʻa i nā hopena kūponoʻole. ʻO kēia kaʻina hana, e hoʻomaʻemaʻe, a hoʻomaʻamaʻa i nā loiloi i hoʻopaʻaʻia mai nā pākeke paʻakikī e hoʻopau ana i nā kūkākūkā e hōʻole i kaʻike data. ʻO ka hoʻohālike kiʻekiʻe loa e hoʻonui ai i ka hoʻowalewale kiʻekiʻe a me ka manaʻo nui,ʻo ia ka mea pono no nā mahina e like me nā mea kūʻai lāʻau lapaʻau, a me nā mea mālama olakino. Hiki i nā kiko'ī liʻiliʻi e like me nā paniʻana o nāʻano Vial e hana i kahiʻokoʻa nui: nā leʻa a iʻole nā mea hōʻeha mai ka salcle ma mua
Key Point: E hoʻohana i nā CHANTIC SPECT SPECHT SPECHT a me SPPTA e pale ai i ka nānāʻana i kaʻikeʻana a me ka hōʻoiaʻana i kaʻike data.
ʻO kahi moku'āina-o-ka-kiʻi lc-ms mea hana i loko o ka hale hana, e hōʻike ana i ka mea nui o ka hoʻomākaukauʻana i ka hana maʻamau.
1. Nā hana maʻamau maʻamau lcms
Kahi
Kumu
Kapalakula
Hoʻopau i nā'āpana (0.2 μm kānana kiʻi) e pale i nā kolamu a me nā nozzles.
Hoʻopiʻi Ikaika Pake
Hoʻohana i nā mea hana kūlohelohe (Acetotitrile, methanotol) e nānā i nā protein pale mai bio-subles.
ʻO ka hana paʻaʻana i ka phase (spe)
Hoʻopili i nā loiloi a wehe i nā interferons e pili ana i nā cardidges sorbenges.
Ka wai wai-wai (lle)
Nā mea kākau e hoʻopaʻa i loko o ka pae kūlohelohe; CESSOTATIVE akā hiki paha iā EMULOSFY.
Kākoʻo i kākoʻoʻia (SED)
Automated lle ma ke kākoʻoʻana i ke kākoʻo; Hōʻalo i ka emilsion a mālama i nā pale.
Derivatization
Hoʻololi ka hoʻololi i nā loiloi (e.g.
Ua hoʻohanaʻia kahi kānana singing sopinge e kānana e kānana i kahi hoʻohālike i loko o kahi volc vial
2. Kāleʻa a me SEPTA: Ke kahu nei nā mea kiaʻi
I LC-MR i nā hana hana,ʻo ka paniʻana o ka VIAL ma mua o ka hoʻopaʻa hopeʻana ma mua o ka nānāʻana. ʻO nā septa kiʻekiʻe a me nā caps e hōʻoia i nā hōʻailona o ka ea, pale aku i ka evaporation a me nā mea hoʻonaninani kūlohelohe.
PTFE \ / Silicone Septa:ʻO nā mea lapaʻau chemically, e hōʻemi ana i nā pulu.
Nā Coast i hoʻopaʻaʻia: Septum mau loa i pili i ka pāpale, hoʻopau i nā'āpana a me ka hōʻoiaʻana i nā hōʻailona maʻamau.
ʻO ka capby kūpono e pili ana i ka hanaʻana i kaʻenehana ʻO kahi hōʻailona palekana, e hoʻomaʻamaʻa i keʻano o ka hanaʻana i ka hana hou a pale aku i ka cross-cross-cross.
ʻO kahi chromatography Autosampler Vial i kūpono me kahi PTFE \ / Silicone Septum a me SPRECHT CAP, e hōʻike ana i kahi hōʻailona Vial no ka LC-MS Sampling.
3.ʻO nā carps caps \ / septa e hoʻomākaukau i nāʻano hoʻomākaukau
Hoʻomaka
Ka paniʻana
No ke aha mai?
Kapalakula
ʻO ka PTFU maʻamau
Ka paleʻana i ka maka i nā pale; Mālama i nā mea e lawe ai i nā mea e lawe ai.
Hoʻopiʻi Ikaika Pake
Pre-slit ptfe \ / silicone septum + screw cap
Hāʻawi i nā hoʻopaʻi ausosampler autosampler me ke kapaʻoleʻole.
Huakai
Pre-slit ptfe \ / silicone septum + screw cap
ʻO nā wikiwiki wikiwiki i hoʻopaʻaʻia; mālama i ke sila i ka wā mālama.
Lle \ / hiamoe
PTFE-faced septum (PTFE \ / Silicone Sandwich) + i ka pāpale pahu
Pale aku i nā pale kū'ē; Mālamaʻia ka hoʻolālāʻana i ka hanaʻana i ka soptum eʻoki ana a iʻole keʻano.
Derivatization
BADED PTFE \ / Silicone Septum + aluminil cap
Hāʻawi i nā hōʻailona lōʻihi e kū'ē i ka hanaʻana a me ka wai.
4. AFIND CAP CAP & SEPTA FACPORS e hoʻohālikelike
'ʻAno'iohi
Waiwai
Nā hiʻohiʻona nui
Noi
Pre-slit ptfe \ / silicone screw car
PTFE \ / Silicone Septum + aluminil Cap
Nā puncture; mister; nā haʻahaʻa haʻahaʻa
ʻO nā LC-OP-OLTTL MC-ms, autosamplers
ʻO ka PTFE maʻamau
PTFE \ / Silicone Septum + aluminil Cap
Ka seal seal laulā; ākea ākea ākea
ʻO nā hana maʻamau lc-ms
ʻO kā DATTUBS Septum CRUP CAP
Al Cap + i hoʻopaʻaʻia septum
Septum i hoʻopili mauʻia; kūlike i ka seal
ʻO nā'ōnaehana automated, mālama lōʻihi
Snap cap + rubber septum
ʻO Rubber Septum + Stainless Steel Clamp
Uku haʻahaʻa; ka hopena o keʻano
ʻO nā kumukūʻai kālā, nā loiloi koʻikoʻiʻole
Papa: Hoʻopiliʻo AIJiren ke pani & septa huahana Profirisson.
Hopena
ʻO ka hopena o LC-MC Ke hoʻohana nei i nā pani kūpono-e like me ka hana mua o AIJIren ma mua o ka hanaʻo AIJIren E pili ana i ka paniʻana i kāu ala e hōʻoia pololei ai, nā hopena hou. No nā mea hana Vetitest
ʻO ke kī kī ʻO ka hoʻomaʻamaʻaʻana o Aijiren
ʻO kahi mea hanaʻoihana hanaʻo Laboratory LC-Ms Autosampler Vials me nā Caps palekana, e hōʻike ana i ka hoʻomākaukauʻana i ka hana maʻamau a me ka laweʻana.
