LCMS Qauv Npaj Ua Si: Txhim Kho Cov Ntaub Ntawv raug nrog Aijiren Caps & Septa
Hauv LC-MS tsom xam, txhua kauj ruam ntawm kev npaj qauv yog qhov tseem ceeb, tshwj xeeb tshaj yog qhov kev xaiv thiab kev siv vial cands thiab septa. Cov lus qhia npaj npaj cov txheej txheem npaj npaj cov txheej txheem tseem ceeb, cov khoom noj nag lossis daus, txhawb nqa cov tshuaj tiv thaiv ua kua, thiab derivatization ua kua. Nws kuj tseem piav qhia yuav ua li cas xaiv Aijiren lub-SLIT PTFE PTFE \ / Silicone sterta, thiab sib dhos cov foob pob ua kom muaj kev nyab xeeb thiab tiv thaiv volatilization thiab paug. Nrog lub rooj khoom lag luam sib piv, cov neeg nyeem tuaj yeem nkag siab qhov zoo thiab daim ntawv teev npe, ua kom muaj qhov tseeb ntawm cov tshuaj, muaj cov proteomics, cov proteomics, thiab kev kuaj mob ib puag ncig.
Lub hauv paus rau phau ntawv
Hauv cov kua chromatography-mass spectrometry (LC-MS), cov qauv kev ua qauv kom raug yog qhov tseem ceeb kom tau txais qhov raug, kom muaj txiaj ntsig zoo. Cov txheej txheem no tawm, purifies, thiab mloog hom phiaj analytes los ntawm complex matrices thaum tshem tawm cov ntaub ntawv ua tau zoo. Cov Qauv Ua Ntej Npaj Ua Ntej yog li txhim kho kev nkag siab thiab qhov tshwj xeeb, uas yog qhov tseem ceeb rau cov liaj zoo li cov tshuaj ntsuam, proteomics, thiab kev kuaj mob ib puag ncig. Cov ntsiab lus me me xws li Vial kaw tuaj yeem ua qhov loj sib txawv: txeej lossis paug los ntawm cov qauv tsis zoo \ / soons yuav hais kom cov qauv ua ntej nws mus txog LC-MS.
Cov ntsiab lus tseem ceeb: Siv Aijiren lub kaus mom ntawm lub ntsej muag thiab septa los tiv thaiv cov qauv kev paug thiab lav tau meej.
Lub xeev-ntawm-cov-kos duab LC-MS cuab yeej hauv chav kuaj, piv txwv txog qhov tseem ceeb ntawm kev npaj ua kom pom tseeb.
1. Cov qauv LCMS npaj ua piv txwv
| Kev txawj | Lub hom phiaj |
|---|---|
| Kev lim | Tshem tawm cov particulates (0.2 long lim) los tiv thaiv kab ntawv thiab lub taub hau. |
| Cov nag lossis daus | Siv cov kuab tshuaj organic (acetoniturile, methanol) kom pom meej cov protein los ntawm cov qauv no. |
| Khoom Theem Extraction (Spe) | Cov tshuaj ntsuam xyuas thiab tshem tawm cov kev cuam tshuam los ntawm kev ua kom pom kev. |
| Ua kua-ua kua extraction (lle) | Kev hloov pauv kev ntsuas rau cov theem organic; Ntau yam tab sis yuav emulsify. |
| Txhawb nqa kua extraction (rau tes) | Rho tawm lle ntawm inert txhawb; zam emulsion thiab txuag cov kuab tshuaj. |
| Derivatization | Chemically hloov analytes (piv txwv li mephees methylation) txhawm rau txhim kho ionization. |
2. Lub kaus mom thiab septa: cov saib xyuas ntawm cov qauv ncaj ncees
Hauv LC-MS workflows, lub vial kaw yog qhov kawg thaiv ua ntej kev tsom xam. Zoo-siab septa thiab caps kom ntseeg tau cov raj huab cua, tiv thaiv kev tiv thaiv evaporation thiab thaiv cov kab mob ib puag ncig.
- PTFE \ / Silicone sterna: chemically inert, txo cov kab ntshiv.
- Lub kaus mom sib raug zoo: septum sterum mus tas li txuas rau lub hau, tshem tawm cov xoob xoob thiab ua kom muaj cov ntsaws ruaj ruaj.
Cov txheej txheem capping kom zoo dua tseem muaj teeb meem: zawm snugly-dhau-zawm-tighting tuaj yeem rhuav seebsta, nyob rau hauv-zawm ua rau ua rau txia. Ib lub cim muaj kev nyab xeeb, cov khoom sib txuam sib xyaw txhim kho coprucibility thiab tiv thaiv kev hla kev sib kis.
3. Cov kaus mom sib xyaw \ / senta los npaj txoj kev
| Kev npaj | Pom zoo kaw | Vim li cas? |
|---|---|---|
| Kev lim | Txheem PTFE \ / Silicone Septum + Tight Ntsia Hlau Cap | Inert lub ntsej muag tiv thaiv cov kuab tshuaj; Tiv thaiv cov partover. |
| Cov nag lossis daus | Pre-sluit PTFE \ / Silicone Septum + Ntsia Hlau Cap | Tso cai rov ua dua AutoSampler punctures tsis muaj cap tshem tawm. |
| Tus kab | Pre-sluit PTFE \ / Silicone Septum + Ntsia Hlau Cap | Ceev cov kev txhaj tshuaj; khaws cov foob thaum cia. |
| Lle \ / / slear | PTFE-ntsib Septum (PTFE \ / Silicone Sandwich) + Briced Cap | Tawm tsam cov kuab tshuaj nruj; Kev tsim qauv tsim qauv tiv thaiv septum hloov lossis o. |
| Derivatization | Bonded PTFE \ / Silicone Septum + Aluminium Cap | Muab cov cim ntev tawm tsam kev tawm tsam kev tawm tsam evaporation thiab dej noo. |
4. Aijiren Cap & Septa Khoom Sib piv
| Khoom Yam | Khoom | Cov Nta Tseem Ceeb | Cov Ntawv Thov |
|---|---|---|---|
| Pre-sluit PTFE \ / Silicone Ntsia Ntsia Hlau Cap | PTFE \ / Silicone Septum + Aluminium Cap | Rov ua dua cov cim; inert; Tsawg Extractables | High-topput lc-ms, autosamplers |
| Txheem PTFE \ / Silicone Ntsia Ntsia Hlau Cap | PTFE \ / Silicone Septum + Aluminium Cap | General-hom phiaj foob; dav hnyav hnyav sib xyaw | Cov LC-MS Vials |
| Sib raug zoo septum crimp cap | Al Cap + Bonded Septum | Septum mus tas li; Xws li Airtight foob | Caw kaw lus, lub sijhawm ntev cia |
| Snap Cap + Roj Hmab Septum | Roj Hmab Septum + Stainless Hlau Clamp | Tus nqi qis; Kev pheej hmoo ntawm kev coring | Pob nyiaj siv-nkag siab, tsis yog-tseem ceeb |
Rooj: Aijiren kaw thiab septa cov khoom sib piv.
Tag
Kev npaj LC-MS qauv yog lub hauv paus ntawm cov ntaub ntawv txheeb xyuas cov ntaub ntawv. Siv txoj cai kaw-xws li Aijiren lub-sreefe ptfe \ / Silicone senta thiab sib khi caps-khaws cov qauv kev ncaj ncees los ntawm kev tiv thaiv kev sib raug zoo thiab evaporation. Sib phim qhov kaw rau koj txoj kev coj ua kom ntseeg tau qhov tseeb, rov ua tau zoo. Rau kev ntsuas (lab kuaj, tshuaj ntsuam R & D, thiab SEPRISED HEEV
Tseem ceeb takeaway: zoo vial cands thiab septa yog qhov tseem ceeb kom tiv thaiv cov qauv poob thiab lav cov kev ntsuas. Aijiren cov kev daws teeb meem kev daws teeb meem LC-MS kev ua tau zoo thiab ntsuas kev ua haujlwm.
