Qhov twg tsis zoo rau koj HPLC Mobile THEEM?
Xaiv txoj cai tsis txaus rau koj HPLC Mobile theem yog qhov tseem ceeb rau kev ua tiav kev sib cais zoo tshaj plaws thiab txhim khu kev qha. Buffers ua si lub luag haujlwm tseem ceeb hauv kev tswj hwm p, uas cuam tshuam ncaj qha cuam tshuam rau kev tuav pov hwm thiab kev xaiv tsa ntawm cov oozable. Nov yog qhov koj yuav tsum xav txog thaum xaiv qhov zoo tshaj plaws tsis zoo rau koj daim ntawv thov.
1️⃣ nkag siab koj cov kws tshuaj ntsuam
Kev xaiv ntawm tsis muaj feem nyob ntawm PKA ntawm koj cov kws tshuaj ntsuam. Qhov tsim nyog, cov theem ntawm koj txawb yuav tsum yog li ntawm ± 1 chav ntawm cov tsis tau muaj peev xwm ua tau muaj peev xwm. Piv txwv li, yog tias koj tus Analyte muaj PKA ntawm 4.5, lub pka phosphate tsis nrog pka nyob ib ncig ntawm tus nqi no yuav haum, muaj ionization xeev.
2️⃣ hom tsis sib xws
Phosphate Buffers: siv dav vim lawv cov txiaj ntsig zoo buffering (Ph 2-8). Lawv tau tshaj nrog ntau tus kws tshuaj ntsuam thiab muab kev ruaj khov zoo.
Acetate Buffers: Tsim nyog rau cov ntawv thov hauv PHQUS BE, feem ntau xws li pH 3.6 mus rau 5.6. Lawv ua tau zoo rau acidic anidaltes.
Tris Buffers: feem ntau yog siv nyob rau hauv cov tshuaj lom neeg, tshwj xeeb rau cov protein, tab sis tej zaum yuav tsis zoo rau UV kuaj tau vim lawv nqus tau.
3️⃣ xav txog kev sib raug zoo
Nco ntsoov tias cov xaiv tsis tau sib haum nrog koj lub HPLC system thiab cov theem chaw nyob ruaj ruaj ntawm koj kem. Qee cov buffers, zoo li centrate, tuaj yeem corrate stainless hlau cais nyob rau lub sijhawm, yog li xav txog cov kev ua kom ntev ntawm koj cov cuab yeej.
4️⃣ Saib Xyuas Kev Tsim Kho
Ib qho kev sib haum xeeb ntawm 25-30 hli yog feem ntau yog siv tau rau feem ntau cov ntawv thov. Kev txiav txim siab dua tuaj yeem txhim kho cov duab siab thiab kev daws teeb meem tab sis kuj tseem tuaj yeem ua rau nce ntxiv rappitation thiab kev kub ntxhov.
