Nkwadebe maka LCSs Purdem: na-agbaso data data na Aijen Subs & Mino
Na nyocha nke LC-MS, nzọụkwụ ọ bụla nke nkwadebe dị oke mkpa, ọkachasị nhọrọ na iji nke vial na prome. Edemede a na-eji usoro nkwadebe dị mkpa, gụnyere ihe nzacha, protein ọdịda, mmịpụta mmiri siri ike, mịrị mmiri mmiri na-akwado, na nmepụta mmiri na-akwado. Ọ na-akọwara otu esi ahọpụta Aninfen pre-slit PTFE \ / silicone propịa, ọkọlọtọ Antonim Capment iji hụ na a na-ejikwa ya dị mma ma gbochie ya. Site na tebụl ngwaahịa atụnyere, ndị na-agụ akwụkwọ nwere ike ịghọta uru na ngwa ọdịdị ọ bụla, na-eme ka ịtate na mmepe data, proomics, na ule gburugburu ebe obibi.
Na mmiri wasmomatography-ukards dị iche iche (LC-MS), nkwadebe ntụle kwesịrị ekwesị dị oke mkpa ịnweta nsonaazụ a pụrụ ịdabere na ya. Usoro a na-ewepụta, kpochasị, ma lekwasị anya na-enyocha site na njikọta mgbagwoju anya mgbe ị na-ewepụ ihe ndabere nke ọma. Ngosiputa ihe nlere anya di elu na nkowa, nke di nkpa maka ubi dika nyocha ogwu, protohomics, na nyocha gburugburu ebe obibi. Obere nkọwa dị ka mmechi mmechi nwere ike ịme nnukwu ọdịiche: na-amịpụta ma ọ bụ ihe mmetọ ma ọ bụ ihe mmetọ ma ọ bụ meghee ga-emebi ihe nlele tupu ya erutela lc-ms.
Isi okwu: Jiri okpu ndi okacha amara Aijari na Septe igbochi nhichapu ma doo m nkwa na ntụkwasị obi data.
Ngwá ọrụ Ngwá ọrụ LC-MS na ụlọ nyocha, na-egosipụta mkpa ọ dị mkpa ịkwadebe nkwadebe.
1. Usoro Ntanetị Ntụle Ntụle
Teknik
Ihe ebu n'obi mee ihe
Eiltration
Na-ewepu ururiga (0.2 μm nzacha) iji chebe kọlụm na nozzles.
Abalị protein
Na-eji sọks organic (acetonitrile, methanol) iji kpochapụ protein na-esite site na ihe nlere.
Mgbapụta ngwa ngwa (SPE)
Ntinye uche ma na-ewepu ihe ntinye site na carridges.
Mwepu mmiri mmiri (LLE)
Na-enyefe na-enyocha n'ime usoro ihe mgbochi; may vasatail ma nwee ike imebi.
A na-akwado mmiri mmiri
Akpaaka na-akpaghị aka na nkwado; na-ezere emulsion ma na-echekwa ntọala.
A na-eji skill na-enyocha ihe atụ n'ime vial vial vial, na-egosipụta nzọụkwụ nzọụkwụ LC-MS.
2
Na LC-MS na-arụ ọrụ, mmechi vial bụ ihe mgbochi ikpeazụ tupu nyocha. Septemba dị elu na okpu dị elu na-ezo aka na airtight airtight, na-egbochi ikuku ma na-egbochi ebe obibi gburugburu ebe obibi.
Mpempe akwụkwọ nke njikọ: Site na Septum aka na okpu, na-ewepụ iberibe oghere ma na-agba mbọ bụrụ akara na-agbanwe agbanwe.
Usoro ihe nlere anya kwesiri ntukwasi-obi di nkpa: ime ka ihe siri ike na-agbachi ca cata, na-eme ka ihe na-eme mkpọtụ. Akara akara nke ntụkwasị obi, edo edo na-eme ka ịmụgharị ma na-egbochi mmerụ ahụ.
Automatographr Val chromatographr dabara na ptfe \ / silicone Seplium na okpu ịghasa, na-egosi akara vial dị nchebe maka nyocha LC-MS.
3. Nkwekọrịta dakọtara \ / Sima na ụzọ ịkwadebe
Njikere
Mmechi a na-atụ aro
N'ihi gịnị?
Eiltration
PTFE \ / Silicone Sepcham + Clash
Legharịa ihu ndị na-eme mkpọtụ; na-egbochi uru na-ebute.
Abalị protein
Pet-slit ptfe \ / salcone Sepum + crans
Na-enye ohere nyocha nyocha nyocha nyocha nke nyocha nke ya na-enweghị ike iwepụ.
Ndị na-emebi ihe iwe; Ntinye uche na-egbochi sepị mgbanwe ma ọ bụ ọzịza.
Ntinye
BTFE \ / Silicone Sepcham + Aluminom okpu
Na-enye oge ogologo oge megide ikuku na mmiri mmiri.
4.
Ụdị ngwaahịa
Mgwooru
Njirimara dị mkpa
Ngwa
Pụrụ Slit PTFE \ / silicone crap okpu
PTFE \ / Silicone Sepum + aliminom okpu
Akara ugboro ugboro; inyocha; Igwe na-enweghị ike
LC-MS, Autosamplers
PTFE \ / silicone crap okpu
PTFE \ / Silicone Sepum + aliminom okpu
Akara zuru oke; Mmekọrịta gabigara ókè
Nkọwa LC-MS Vals
Ezi okpu nkebi
Al okpu +
Septum agbakọtara kpamkpam; akara ikuku
Sistemụ akpaaka, nchekwa ogologo oge
Mpempe akwụkwọ
Mpempe akwụkwọ Mpempe akwụkwọ Mpekere
Ọnụ ala; ihe egwu nke isi
Ntughari uche di iche iche, ndi n'enweghi nsogbu
Table: Aijiran Mechie & ntụnyere nke sepra ngwaahịa.
Mmechi
Nkwadebe nlele LC-MS dị irè bụ ntọala nke data nyocha sitere na ya. Iji mmechi ziri ezi - dịka nke Aninfen PTFE \ / silicone capra na-echekwa iguzosi ike n'ezi ihe site na igbochi mmetọ na evaporation. Dakọtara mmechi ahụ na usoro gị na-eme ka nsonaazụ ziri ezi. Maka nyocha nyocha nke nyocha, ọgwụ na-eme nyocha R & D, na-ahọrọ okpu Aijian dị elu na sensa, nsị, irighiri ihe) ma na-akwado.
Isi Takeaway: Oke nke dị mma na Sepda dị mkpa iji gbochie nlele ma na-ekwe nkwa nyocha. Achịcha AIJiren's akara dị ka arụmọrụ LC-MS na-arụ ọrụ na arụmọrụ.
Onye na-ahụ maka nyocha nke LC-ms na-ejikwa Vals Autosampler LC-MS na Casts echekwara, na-egosi iji nlezianya tụlee nkwadebe na njikwa.
