Kukonzekera kwa LCMS: Kuwonetsetsa kulondola kwa data ndi Aijiren Capus & Septa
Mu kusanthula kwa LC-MS, kukonzekera kulikonse ndikofunikira, makamaka kusankha ndi kugwiritsa ntchito zisoti za vial ndi septa. Nkhaniyi yamawu imakwirira njira zazikulu zokonzekera, kuphatikizapo kuchuluka kwa pro protetion, gawo lolimba, kuchotsera madzi, kumathandizira madzi, komanso kuthandizidwa. Ikufotokozanso momwe mungasankhire ptfire ptfer ptfe \ / slicone septa, septa, ndi zipilala zolumikizidwa ndi ma aluminiyamu ogwiritsira ntchito zoyeserera zosiyanasiyana kuti muwonetsetse kuti zisindikizo zotetezedwa komanso kuipitsidwa. Ndi tebulo lazofalitsidwa, owerenga amatha kumvetsetsa bwino zabwino ndi malo ogwiritsira ntchito a kapu iliyonse \ / kuphatikizira mtundu waukulu wa chitukuko ndi kulondola kwa deterics, komanso kuyezetsa zachilengedwe.
Chiyambi
Mu madzi a chromatography - misa spectrometry (LC-MS), Kukonzekera koyenera ndikofunikira kuti mupeze zotsatira zolondola, zodalirika. Njirazi zimatulutsa, kutsuka, ndi kufufuza kwandamale chandamale kuchokera ku matricts poletsa zosokoneza zomwe zimapangitsa kuti zisokonezeke. Makasitomala apamwamba pokonzekera chidwi ndi kusandulika, zomwe ndizofunikira kwa minda ngati mankhwala opanga mankhwala, mapulotekeni, komanso kuyezetsa zachilengedwe. Zambiri zazing'ono monga kutsekera kwavizi zitha kupanga kusiyana kwakukulu: kutayikira kapena chidebe chochokera ku Capss \ / SEPTA idzasokoneza chitsanzocho chisanafike mpaka LC-MS.
Mfundo zazikuluzikulu: Gwiritsani ntchito akatswiri a Aizereren ndi Septa kuti mupewe kuipitsidwa ndi kutsimikizira kukhulupirika kwa data.
Boma la LC-Art mu labotale, pofotokoza kufunika kwa zitsanzo za zitsanzo za zitsanzo.
1. Njira zofala zofala za LCMS
| Kachitidwe | Cholinga |
|---|---|
| Kufinya | Chotsani tinthu tating'onoting'ono (0,2 μm) kuteteza mzati ndi nozzles. |
| Protein mpweya | Amagwiritsa ntchito ma sodic okhazikika (acetonitrile, methanol) kupita ku mapuloteni ochokera ku Bio-zitsanzo. |
| Gawo lolimba m'zigawo (Spe) | Zosakanikirana amasanthula ndikuchotsa zokambirana kudzera pa matotoni azovala. |
| Madzi-madzi amadzaza (LLE) | Kusandulika kusanthula gawo lakale; mosiyanasiyana koma atha. |
| Adachirikiza madzi a madzi (adagona) | Zochita zokhazokha panthambi zimathandizira; amapewa emulsion ndikusunga ma sol sol. |
| Kuleka | Mankhwala amadzisintha (E.g.) kuti musinthe ma ionization. |
2. Zipewa ndi Septa: Oyang'anira Chitsanzo
Mu LC-MS amakola, kutsekedwa kwabial ndi cholepheretsa komaliza kusanthula. Septa yapamwamba kwambiri ndi zisoti zotsimikizika zimatsimikizira zisindikizo zam'madzi, kupewa kusinthasintha ndikuletsa zodetsa zachilengedwe.
- PTFF \ / Silicone septa: mogwirizana ndi mankhwala, kuchepetsa miyendo.
- Zipewa Zogwirizanitsa: Septum yolumikizidwa kwathunthu ku kapu, kuchotsa zidutswa zomasulidwa ndikuwonetsetsa zisindikizo zokhalapo.
Njira Yosakankhira Zoyenera: Mangitsani Kulimbana Kwambiri Kwambiri Kutha Kukuta Septa, Kuwala Kwambiri kumayambitsa kutayikira. Chisindikizo choteteza, yunifolomu chimawongolera kubereka ndipo chimalepheretsa kuipitsidwa.
3. Zithunzi zofananira \ / Septa kuti mukonze njira
| Kukonzekela | Kukhazikitsidwa kutsekedwa | Chifukwa chiyani? |
|---|---|---|
| Kufinya | \ \ / yicone septum + wolimba screm | Chotsani masana amasungunuka; amalepheretsa kunyamula. |
| Protein mpweya | Pre-slit ptfe \ / silicone septum + screw cap | Amalola kubwezeretsanso zotukwana popanda kuchotsedwa kwa cap. |
| Sing'anga | Pre-slit ptfe \ / silicone septum + screw cap | Liwiro jakisoni; imasunga chisindikizo panthawi yosungira. |
| Lle \ / adagona | Ptfe-Fatdd Septum (PTF \ / Swicone sangweji) + chipewa | Amakana zisungunuke za usungunuke; Makina ogwiritsidwira ntchito amalepheretsa kusintha kwa septum kapena kutupa. |
| Kuleka | Wolumikizidwa PTFF \ / silicone septum + aluminium cap | Imapereka chisindikizo cha nthawi yayitali motsutsana ndi kunyowa. |
4..
| Mtundu Wogulitsa | Malaya | Mawonekedwe Ofunika | Mapulogalamu |
|---|---|---|---|
| Pre-slit ptfe \ / silika screw cap | Ptfe \ / silicone septum + aluminium cap | Kubwerezedwa mobwerezabwereza; rt; Zowonjezera zotsika | Ed-Rusting LC-MS, Autosamursrs |
| \ \ / yicone screw cap | Ptfe \ / silicone septum + aluminium cap | Chisindikizo chachikulu; Kugwirizana Kwambiri | Routine LC-MS MS |
| GUPTME HUPPP CARP | Al Cap + womangidwa septum | Septum yolumikizidwa kwathunthu; Chisindikizo chosasintha | Makina Okhawo Okhazikika, Kusunga Kwakutali |
| Snap Cap + Steptum | Nsanja ya mphira + yosapanga dzimbiri | Mtengo wotsika; chiopsezo cha kutchinga | Kusanthula bajeti, osasamala |
Gome: Aijaren otsetsereka & Septa Centrison.
Mapeto
Kukonzekera kwa LC-MS PSS ndikukonzekera ndi maziko a chidziwitso chodalirika. Kugwiritsa ntchito zotsekera kumanja - monga chisanalo cha aijiren ptfe \ / zikondwerero za Silicone Septa ndi zogwirizanitsa, zimasunga umphumphu, ndikusunga umphumphu popewa kuipitsidwa ndi kutuluka. Kufananitsa kutsekedwa kwa njira yanu kumatsimikizira zotheka, zobereka. Kwa kafukufuku wa lab, mankhwala ogulitsa a Prermaceutical R & D, ndi maphwando achitatu a Labs, kusankha malo apamwamba kwambiri ndi sepsa kumathetsa zopweteka (kutaya, kutaya kodalirika kwa LC-MS.
Positi Yapamwamba: Zipangizo zabwino za vial ndi Septa ndizofunikira kuti mupewe kutaya kwake ndikutsimikizira mwadzidzidzi. Njira zothetsera Agiren zimayambitsa LC-MS magwiridwe antchito ndi ntchito zolimbitsa thupi.
