Ndi buffer iti yomwe ili bwino kwambiri kwa gawo lanu la HPLC?
Kusankha Buffer yoyenera kwa gawo lanu la HPLC ndikofunikira kuti mukwaniritse zopinga zokhazikika komanso zotsatira zabwino. Zimakhala ndi gawo lalikulu pakuwongolera pH, zomwe zimakhudza mwachindunji kusunga ndi zomwe zimayambitsa chidwi ndi chidwi, makamaka iwo omwe ali oona. Izi ndi zomwe muyenera kuganizira mukamasankha wolipidwa wabwino kwambiri kuti mugwiritse ntchito.
Kumvetsetsa kwanu
Kusankha kwa Buffer kumadalira pa PKA za Openda. Zoyenera, pH ya gawo lanu lam'manja liyenera kukhala mkati mwa ± 1 unit ya PKA ya Buffer's PKA kuti muwonetsetse kukhala koyenera. Mwachitsanzo, ngati kafukufuku wanu ali ndi PKA ya 4.5, buffer ya phosphate yokhala ndi pka yozungulira mtengo uwu ungakhale woyenera, ndikukulolani kuti musangalale ndi boma lomwe mukufuna.
Mitundu iwiri ya Buffer
Bulute Buffer: Kugwiritsidwa ntchito kwambiri chifukwa cha kuchuluka kwake kwamphamvu (ph 2-8). Amagwirizana ndi owunikira ambiri ndikupereka bata labwino.
Zolemba za Acetate: Zoyenera mafo otsika, nthawi zambiri kuyambira pa pH 3.6 mpaka 5.6. Ndiwothandiza kwa acidic acidic.
Tris Bucebs: Nthawi zambiri amagwiritsidwa ntchito pazinthu zachilengedwe, makamaka pamapuloteni, koma sizingakhale bwino kuzindikiridwa kwa UV chifukwa cha kuyamwa kwawo.
3️⃣ Onani Kugwirizana
Onetsetsani kuti buffer yosankhidwa imagwirizana ndi dongosolo lanu la HPLC ndi gawo la mzere wa mzati wanu. Zina zimaphuka, monga citrate, zimatha kusenda dzimbiri kwakanthawi, choncho lingalirani za nthawi yayitali pazida zanu.
4 ️⃣ kuwunika
Kukhazikika kwa 25-50 mm nthawi zambiri kumathandiza pa ntchito zambiri. Kukhazikika kwakukulu kumatha kukulitsa mawonekedwe ndi chiwerengero chosintha komanso chingapangitsenso kuchuluka kwa zakumbuyo komanso kuthekera kwa mpweya.
