Ndeipi buffer yakanakira yako HPLC mobile chikamu?
Kusarudza iyo chaiyo buffer yeiyo HPLC nhare mbozha yakakosha ndeye yakakosha kuwana kupatsanurana kwakakwana uye kwakavimbika mibairo. Buffers anotamba yakakosha basa mukudzora pH, iyo inokanganisa zvakananga kuchengetwa uye nekukwezva kwekuongorora, kunyanya izvo izvo zvinofungwa. Izvi ndizvo zvaunoda kufunga kana uchisarudza zvakanakisa buffer kune yako application.
1Nonzwisisa Ongorora Wako
Sarudzo yeBuffer inonyanya kuenderana nePTO yewako Ouku. Zvakakodzera, iyo pH yefoni yako yefoni inofanira kunge iri mukati me1 1 yuniti yeBuffer's PTA PDA kuti ive nechokwadi chekubudirira kugona. Semuenzaniso, kana ana anawate yako paPT 4,5, phosphate buffer ine pka yakatenderedza kukosha uku yaizokodzera, ichikubvumira kuti urambe uine ana aumbio yazvino.
2️⃣ Buffer mhando
Phosphato buffers: yakashandiswa zvakanyanya nekuda kweiyo yavo yekubika buffering range (ph 2-8). Ivo vanoenderana nevakawanda vanoongorora uye vanopa kugadzikana kwakanaka.
ACETETA BUFFERS: Inokodzera Pasi Pe SP APL application, kazhinji kubva paPH 3.6 kusvika 5.6. Ivo vanoshanda kune acidic ongororo.
Tris buffers: inowanzoshandiswa muzvinyorwa zvekushandisa, kunyanya nemapuroteni, asi inogona kunge isina kukodzera kuongororwa kweUV nekuda kwekutora kwavo.
3️⃣ Funga nezveKupokana
Ita shuwa kuti iyo yakasarudzwa buffer inoenderana neyako HPLC system uye chiteshi chekamuri yako. Vamwe buffers, vakaita seCitrate, vanogona kugarisana vasina masimba masimbi enguva yekufamba kwenguva, saka funga nezvemhedzisiro refu-refu pamidziyo yako.
4️⃣ Tarisa Buffer Confentration
Kusunganidzwa kwe25-50 mm kazhinji kunoshanda kune akawanda mashandisirwo. Nzvimbo dzakakwirira dzakakwirira dzinogona kusimudzira chimiro chepamusoro uye resolution asi zvinogona zvakare kukonzera kuwedzera kudzoka uye kungangoitika.
