Litokisetso tsa li-LCMS tsa LCMS: Ho netefatsa ho nepahala ha data ka li-cap tsa veine & Septa
Ka tlhahlobo ea LC-MS, mohato o mong le o mong le o mong le o mong le o mong oa ho itokisa e bohlokoa, haholo khetho le tšebeliso ea li-cap tsa valta le Septa. Sengoliloeng sena se hlophiseha ka mokhoa o hlophisetsang mekhoa ea ho itokisetsa ka nepo, ho kenyelletsa le ho fetoha ha lipalesa, ho tsoaloa ha profete e tiileng, ho tsoaloa ha metsi, ho tlosoa metsi a mangata. E boetse e hlalosa mokhoa oa ho khetha ID'T ea Ajiro \
silicone septa, le li-caps tse tloaelehileng bakeng sa tiiso e fapaneng le ho thibela ho qhekella le ho hlekefetsoa. With a comparative product table, readers can clearly understand the advantages and application scenarios of each cap\
septa type, enabling greater reproducibility and data accuracy in pharmaceutical development, proteomics, and environmental testing.
Ka li-chromatography-tsa li-spearctrometry (LC-MS), mohlala o nepahetseng oa mohlala, ho ikhopolla ho bohlokoa ho etsa hore ho be le litholoana tse nepahetseng le tse tšepahalang. Ts'ebetso ena e ile ea eketsa, e hloekisoa, 'me e bua ka litlhahiso tse rarolloang ho tsoa maphetetso a rarahaneng ha a ntse a tlosa boleng ba data. High-quality sample prep thus enhances sensitivity and specificity, which is essential for fields like pharmaceutical analysis, proteomics, and environmental testing. Lintlha tse nyane joalo ka ho koaloa ha mapheoana li ka etsa phapang e kholo: li tsoa ho li-cap tse futsanehileng \
Septo li tla sekisetsa sampole pele se fihla LC-Ms e fihla LC-Ms e fihlang LC-Ms.
Polelo ea bohlokoa: Sebelisa lika-rang tsa setsebi sa Airen le Septa ho thibela tšilafalo ea mohlala le ho netefatsa data ea data.
Semelong sa MS-O-ly-Tsepatsang ka laboratoring, se bontša bohlokoa ba tokiso e loketseng.
1. Li-croms tsa li-lCM tsa li-lcms tsa procs
Mokhoa
Morero
Phetetso
E tlosa likarolo (li-filthara tse 0,2 μm) ho sireletsa likholomo le li-nozzles.
Pula ea protheine
E sebelisa li-sholvents tsa khale (acetonitrole, methanol) ho hlakola liprotheine tse tsoang ho sampole ea bio-sampole.
Tlhahiso e tiileng ea Quse (SPE)
A shebisisa litlhahlobo mme o tlosa litšitiso ka mabokose a kokotang.
Ntlha e nang le metsi a metsi (000)
Liphetoho tse fetohang ho ea pele ka karolo ea manyolo; qabang empa e ka hlakoloa.
Ho ntšetsa pele ho sitisoa ka metsi (sle)
E ikemetseng e ikemetseng ho litšehetso tse kahare; Qoba emulsion le ho boloka li-solvent.
Semolao
Ka lik'hemik'hale e fetola lihlahla (e.g. Methylation) ho ntlafatsa ionization.
Sethala sa syringe se sebelisetsoa ho taka sampole ho sa tloaelehang ho Hplc se etsa hore mohato o tloaelehileng oa mohlala oa LC-MS.
2. Caps le Septa: bahlokomeli ba Bohato ba Mohlala
Ho LC-MS Workflows, ho koaloa ha vial ke tšitiso ea ho qetela pele ho hlahlojoa. Septa ea boleng bo holimo le li-cap li netefatsa litirous litimation ea Airlight, li thibela li-tis tsa airlight, li thibela li-tir tsa airlight, le ho thibela lits'oants'o tsa tikoloho ea tikoloho.
PTF \
silicone SEPTA: ho kenella ka lik'hemik'hale, ho fokotsa lekhabunyane.
Li-cap tse tlatsitsoeng: Septume e khomaretse ruri, o felise likoto tsa ho hlekefetsoa ebe o netefatsa litiiso tse mpe.
Mokhoa o nepahetseng oa ho hlapaola le oa bohlokoa: o tiisa ka boomo, o ka tiisa Septa, e tieang tlasa lisosa tsa ho tloha. Tiiso e sireletsehileng, u junifong e ntlafatsoa e hlahisa tlhahiso le ho thibela tšilafalo ea sefapano.
Petrotography appler e lekantsoeng ka septum ea ptfe \
3. Li-caps tse tšoanang \
Septa ho Mekhoa ea ho Lokisa
Ho Itokisetsa
Ho koala ka ho koaloa
Hobane'ng?
Phetetso
Standard PTFE \
Slicon Septum + Tight Screw Cap
Inthrt sefahleho sa ho qala lithupa; e thibela likarolo tsa lintho tse tšoanang.
Pula ea protheine
Pre-slit ptfe prefe \
Slicon Septum + Screw Cap
E lumella li-punctures tse phetoang li sa natse maikutlo ntle le cap.
Spe
Pre-slit ptfe prefe \
Slicon Septum + Screw Cap
Lebelo la ente; e boloka tiiso nakong ea polokelo.
\
sle
Liptum tsa tlhaho tsa PTFE (PTFE \
SIGICON sandwich) + cap cap
Ha a hasanya li-solvent tse mabifi; Moralo o tlamiloeng o thibela septum e feto-fetoha kapa ho ruruha.
Semolao
Boted ptfe \
silicone Septum + Aluminium cap
E fana ka tiiso ea nako ea nako e telele khahlanong le iteress ea mouoane le mongobo.
4. Aijuren Cap & Septa Produshe
Mofuta oa Lihlahisoa
Lintho tse bonahalang
LINTHO TSE HLAHANG
Likopo
Pre-slit ptfe prefe \
silicone screw cap
PTF \
Slicon Septum + Aluminium cap
Pherekano e pheta-phetoang; Inert; Litšitiso tse tlase
Phahameng-conb-ms, li-autosamplers
Standard PTFE \
silicone screw cap
PTF \
Slicon Septum + Aluminium cap
Se tiiso ea ka kakaretso; Khokahano ea Solive e pharalletseng
Mokhoa oa RC-MS Vials
Septum ea septum climp
Al cap + ho bolaoa septum
Septum e khomaretsoe ruri; tiiso e sa fetohe
Litsamaiso tse ikemetseng, polokelo ea nako e telele
Snap cap + Rubber Septum
Rabar Septum + tsitsipano ea tšepe
Theko e tlase; Kotsi ea Cling
Bahlahlobisisi ba lichelete, ba sa tsotelleng
Tafole: Ho koaloa ha sehlahisoa le Septa Probin ha e bapisoa.
Sephetho
Litokisetso tsa LC-MS-MS ke motheo oa lintlha tse tšepahalang tsa tlhahlobo e tšepahalang. U sebelisa mekoallo e nepahetseng - joalo ka li-a li-appa tsa ajiuren pre-\
siricone Septa le li-cap tse tlamiloeng - tse bolokang Botšepehi bo ka Bonang le Bofelo. Ho bapisa ho koaloa ho OPO ea hau ho tiisa litholoana tse nepahetseng, tse khothatsang. Bakeng sa bahlaseluoa ba Lab, Pharmaceutical R & D, le makhulo a batho ba boraro, ho khetha lintlha tse tloaelehileng tsa moea (li khetha libaka tse tloaelehileng tsa bohloko
Ntho ea bohlokoa ea bohlokoa: Li-cap tsa boleng bo boholo le Septa li bohlokoa ho thibela tahlehelo ea mohlala le ho netefatsa ho nepahala ha mohlala. Aijiurn's Tinating Songment Elevate LC-MS Ts'ebetso le Ts'ebetso ea Laboratori.
Setsebi sa senotlolo sa laboratoriki se tsamaisang liphaephe tse tharo tse nang le li-cap tse sireletsehileng, tse bontšang ho lokisa sampole le ho sebetsana le 'moho.