I-LCMS yesampulu yesampulu: Ukuqinisekisa ukuchaneka kwedatha kunye ne-caps ye-Aijiren & Septa
Kuhlalutyo lwe-LC-DS, onke amanyathelo akwamalungiselelo eesampulu kubalulekile, ngakumbi ukhetho kunye nokusetyenziswa kwe-vial caps kunye neSepta. This article systematically covers key preparation techniques, including filtration, protein precipitation, solid phase extraction, liquid–liquid extraction, supported liquid extraction, and derivatization. Ikwacacisa indlela yokukhetha i-Aijiren Slit Pl-Slit Pl-Slit \ /licone Septa, i-Septa esemgangathweni ye-Aluminam ye-Conflow yokuSebenzisa itywina kunye nokuthintela ingqungquthela kunye nongcoliseko. Ngetafile yemveliso yokuthelekisa, abafundi banokuziqonda ngokucacileyo iingenelo kunye neemeko zesicelo zeCAPTA yeCAPTA ,.
Intshayelelo
Kwi-chromatography-Mass Spectrametry (LC-MS), ukulungiselela isampulu efanelekileyo kubalulekile ekufumaneni iziphumo ezichanekileyo nezinokuthenjwa. Le nkqubo isusa, ihlanjuluza, kwaye igxile ekuhlalutyeni kwimatriki entsokothileyo ngelixa kuphelisa intembeko esemgangathweni. I-PREPE-IPUPE SPUPROPE PRORE PREPE iphucula ubuntununtunu kunye nobumnene, nto leyo ebalulekileyo kwimihlaba efana nohlalutyo lwamayeza, iproteyuli kunye novavanyo lokusingqongileyo. Iinkcukacha ezincinci ezinjengokuvalwa kwe-Vial zingenza umahluko omkhulu: Ukuvuza okanye ukungcola kwe-capt \ / septa kuya kuyibeka kwimeko ngaphambi kokuba kufike i-LC-ms.
Inqaku lesitshixo: Sebenzisa i-caps yobungcali ye-Aijhiren kunye ne-sepga ukuthintela ungcoliseko lwesampulu kunye nokuqinisekisa ukuthembeka kwedatha.
Isixhobo se-LC-so-sort LC-MS kwilabhoratri, ibonisa ukubaluleka kwelungiselelo lesampulu ye-meticuus.
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| Ubuchule | Injongo |
|---|---|
| Ukuthoba | Susa iinkcukacha (0.2 μm ifilitha) ukukhusela iikholamu kunye nee-nozzles. |
| Iprotein Primift | Sebenzisa izinyibilikisi ze-organic (i-acetonitrile, methanol) kwiiproteni ezivela kwiisampulu ze-bio. |
| Ukukhutshwa kwesigaba esiqinileyo (Spe) | Ukugxininisa uhlalutyiweyo kwaye ususe intengiselwano nge-sortridges ye-sorble. |
| Ukukhutshwa kwe-windows-windows (LE) | Udluliselo luhlalutya kwiSigaba semisebenzi; Ukuguquguquka kodwa kungalingisa. |
| Ukunikezelwa kwe-fluel | I-Automated LE kwi-InErt inkxaso; kuthintela imvelaphi kwaye igcina izinyibilikisi. |
| Ukugxothwa | Ukulungisa ngokucacileyo uhlalutyo (i.G. METHYIRT) ukuphucula u-Iorses. |
I-2. I-CAPS kunye neSepta: Abagcini beSimo sesampulu
Kwi-LC-MS yokuSebenzisa i-DOORDOWS, ukuvalwa kwe-vanus sisithintelo sokugqibela ngaphambi kohlalutyo. I-Septa esemgangathweni ephambili kunye ne-CAPS iqinisekisa amatywina anomoya, ukuthintela ukuvela nokuvalwa kokungcoliseka kwendalo.
- I-ptfe \ / Isiliki septa: Inertly i-hemiches, ukunciphisa ungenelelo.
- I-COPPSECS: I-Septum iqhotyoshelwe kwi-cap, isusa iziqwenga ezikhululekileyo kunye nokuqinisekisa amatywina aguquguqukayo.
Indlela efanelekileyo yokuqhubela phambili ikwabalulekile Itywina elikhuselekileyo nelingalinganiyo liphucula ukuzala kwakhona kwaye kuthintela ungcoliseko olunqamlezileyo.
I-3. Ukudibanisa i-CAPS \ / Septa kwiindlela zokulungiselela
| Ukulungiselela | Ukuvalwa okucetyiswayo | Ngoba? |
|---|---|---|
| Ukuthoba | I-ptfe \ ptfe \ / isiliki septum + i-screw cap | Ubuso be-Istist buchase izinyithisi; thintela iinkcukacha ze-canteover. |
| Iprotein Primift | I-pre-slit ptfe \ / i-silicone septum + i-screw cap | Ivumela ukuphoswa kwamaxesha aphindaphindiweyo. |
| Nsi | I-pre-slit ptfe \ / i-silicone septum + i-screw cap | Isantya inaliti ezenzekelayo; Gcina itywina ngexesha lokugcina. |
| Lle \ / | I-PTFE-I-PTFE-I-PTFE \ / I-SISiline Sandwich) + cap cap | Baxhathisa izinyibilikisi ezinoburharha; Uyilo olucekeceke uthintela okanye ukudumba. |
| Ukugxothwa | I-ptfed ptfe \ / isiliki septum + i-aluminium cap | Inika itywina lexesha elide ngokuchasene ne-Eaporation kunye ne-Inress. |
I-4. I-Aiiren Cap & Septa Imveliso yeNdawo
| Uhlobo lwemveliso | Izixhobo | Ezona mpawu | Izicelo |
|---|---|---|---|
| I-pre-slit ptfe \ ptfe \ / sisikroba se-silicone cap | Ptfe \ / isiliki septum + i-aluminium cap | Ukuphindaphinda ukubhomboloza; I-InErt; Iphantsi | Igalelo eliphezulu lc-ms, i-autosamplers |
| I-ptfe eqhelekileyo | Ptfe \ / isiliki septum + i-aluminium cap | Itywina ngokubanzi; Ukulungelelaniswa ngokubanzi | I-vials ye-LC-NS |
| I-Conptum ye-septum cruc | I-al cap + ye-septum | I-Septum iqhotyoshelwe ngokusisigxina; Itywina le-airtight | Iinkqubo ezenzekelayo, ukugcinwa kwexesha elide |
| I-Snap cap + irabha ye-septum | I-Subber Septum + I-Staini ye-Staill | Ixabiso eliphantsi; umngcipheko wokulungisa | Uhlahlo-lwabiwo mali olunolwazi, olungenangqondo |
Itheyibhile: Ukuvalwa kwe-aiiren kunye ne-Septa.
Ukuqukumbela
Ukulungiswa kwesampulu ye-LC-MS yesampulu sisiseko sedatha ethembekileyo yohlalutyo. Sebenzisa ukuvalwa kwelungelo elingaphandle-njenge-aijiren slit ptfe \ /licone Septa kunye ne-capta ye-chephu-igcina ingqibelelo yesampulu ngokuthintela ungcoliseko kunye nokuphuma. Ukutshatisa ukuvalwa kwendlela yakho kuqinisekisa iziphumo ezichanekileyo, ezihlawulwayo. Kumhlalutyi weelebhu, amayeza e-PAMMACEUCATION R & D, kunye ne-Lebs yeqela leqela le-Aijiren, i-Septa SOSTA SORS (ilahleko, ilahleko ye-LC-MS) kwaye ixhasa ilahleko ye-LC-ms.
Iqhosha lokuthatha: I-COPS ye-Vial ye-Vial kunye neSepta ibalulekile ukuthintela ilahleko yesampulu kunye nokuqinisekisa ukuchaneka kohlalutyo. Izisombululo zetywina ka-Aijiren ziphakamisa intsebenzo ye-LC-ms kunye nelebhu yokusebenza kwelebhu.
