Yeyiphi i-buffer ilungele isigaba sakho se-HPLC?
Ukukhetha i-buffer efanelekileyo kwisigaba sakho se-HPPC sibaluleke kakhulu ekufezekiseni ulwahlulelo olufanelekileyo kunye neziphumo ezinokuthenjwa. I-Buffers idlala indima ebalulekileyo ekulawuleni iPP, echaphazela ngokuthe ngqo ukugcinwa kunye nokutsala kwabo, ingakumbi abo banazi. Nantsi into ekufuneka uyiqwalasele xa ukhetha i-buffer efanelekileyo yesicelo sakho.
1️⃣ Qinisa uhlalutyi bakho
Ukukhetha i-buffer ikakhulu kuxhomekeke kwi-PKA yohlalutyo. Ngokufanelekileyo, i-PH yenqanaba lakho leselfowuni kufuneka libe ngaphakathi kwiCandelo le-1 le-Buffer's Pka ukuqinisekisa amandla okusebenza okunempumelelo. Umzekelo, ukuba uhlalutyo lwakho lune-SKA ye-4.5, i-phosphate buffer ene-APKA ngeli xa ixabisekileyo, ikuvumela ukuba ugcine uhlalutyo kwimeko yayo oyifunayo u-Ionis.
2️⃣ Iindidi ze-buffer
I-phosphate buffers: isetyenziswa ngokubanzi ngenxa yoluhlu lwabo lwe-buffting (PH 2-8). Zihambelana nabahlalutyi abaninzi kwaye banike uzinzo oluhle.
I-Acetate Buffers: Ifanelekile kwizicelo ze-PH, zihlala zivela kwi-PH 3.6 ukuya ku-5.6. Zisebenza kakuhle kuhlalutyo lwe-acidic.
I-Tris Buffers: isetyenziswa kwizicelo zebhayoloji, ngakumbi iiproteni, kodwa zisenokungabilungelanga ukufunyanwa kwe-UV ngenxa yokungena kwabo.
3️⃣ Qwalasela
Qinisekisa ukuba i-Buffer ekhethiweyo iyahambelana nenkqubo yakho ye-HPLC kunye nesigaba sekholamu yakho. Ezinye ii-buffers, njenge-cittery, zinokulungisa amacandelo eentsimbi ezingahambiyo ngokuhamba kwexesha, ngoko ke cinga ngeziphumo zexesha elide kwizixhobo zakho.
4️⃣ jonga uxinzelelo lwe-buffer
Ukuxinezeleka kwe-25-50 mm iqhele ukusebenza kakuhle kwizicelo ezininzi. Uxinzelelo oluphezulu lunokuphucula ubume be-Peak kunye nesigqibo kodwa kunokukhokelela ekunyukeni umva kunye nokubuyela umva.
