I-HPLC vs. GC-MS: Yikuphi inqubo okufanele uyikhethe?
Lapho unquma phakathi kwe-chromatography ephezulu ye-chromatography (HPLC) kanye negesi ye-chromatography-mass spectrometry (GC-MS), kubalulekile ukuqonda amandla kanye nokusetshenziswa kwendlela ngayinye. Nasi isibukezo sokuqhathanisa ukukusiza wenze isinqumo esinolwazi.
Umehluko Osemqoka
Isigaba seselula:
I-HPLC isebenzisa isigaba se-liquid mobile, okwenza ilungele amakhompiyutha ahlukahlukene, kufaka phakathi izinto zePolar nezengezona eziguqukayo.
I-GC-MS isebenzisa isigaba se-Gaseous Mobile Mobile, ilungele amakhompiyutha aguqukayo angahlehlisa ngaphandle kokubola.
Izinhlobo zesampula:
I-HPLC iguquguqukayo, iyakwazi ukuhlaziya amakhamiya, izingxenye zokudla, kanye nama-biomolecule.
I-GC-MS Excels ekuhlaziyeni amakhompiyutha ajwayelekile ezinto ezi-organic (ama-Vocs), ama-hydrocarbons, namasampula wezemvelo.
Izimo Zokushisa:
I-HPLC isebenza kumazinga okushisa akhona noma aphakanyisiwe, alondoloze amakhompiyutha acwebezelayo.
I-GC-MS idinga amazinga okushisa aphezulu (imvamisa cishe ngo-150 ° C) ukuqinisekisa i-vaporization yesampula.
Izindlela zokutholwa:
I-HPLC imvamisa isebenzisa izindlela zokutholwa ezingeyona eyonakalisayo njenge-UV \
Viza iSpectroscopy.
I-GC-MS ihlanganisa i-Gogesi i-Chromatography nge-Mass Spectrometry yokuhlonza okuningiliziwe okunemininingwane nokuthathwa.
Ukusebenza kwezindleko:
Ngokuvamile, i-GC-MS isebenza kakhulu ngenxa yezindleko eziphansi zokusebenza ezihambisana negesi ngokuqhathaniswa nezinto ezibizayo ezidingekayo kwe-HPLC.
Izinja
Sebenzisa i-HPLC ye:
Ukuhlaziya izingxube eziyinkimbinkimbi kuma-Pharmaceuticals kanye neSayensi Yokudla.
Uphenya ama-biomolecule kanye nezinto ezikhanyiselwe kahle.
Khetha u-GC-MS lapho:
Ukusebenza namakhompiyutha aguqukayo ekuhlaziyeni kwemvelo noma izimboni ze-petrochemical.
Ukuhlaziywa okuningiliziwe okuningiliziwe kuyadingeka ekuhlonzeni okuyikho.