Ukulungiswa kwesampula ye-LCMS: Ukuqinisekisa ukunemba kwedatha nge-Aijiren Caps & Septa
Ekuhlaziyweni kwe-LC-MS, zonke izinyathelo zokulungiselela amasampula zibalulekile, ikakhulukazi ukukhetha nokusetshenziswa kwama-vial cap kanye ne-septa. Le ndatshana ifaka ngokuhlelekile amasu wokulungiselela asemqoka, kufaka phakathi ukuhlunga, i-protein yezulu, ukukhishwa kwesigaba esiqinile, ukukhishwa okuwuketshezi okuwuketshezi, ukukhishwa koketshezi okusekelwe. Ibuye ichaze nokuthi ungazikhetha kanjani i-Aijiren's Pre-slit PTFE \ abicone Septa, i-Senta ejwayelekile ye-Senta, kanye ne-Bonded Aluminium CAPS yokuhamba komsebenzi ohlukile ukuze uqinisekise uphawu oluvikelekile futhi uvikele ukuguquguquka nokungcola. Ngethebula lomkhiqizo wokuqhathanisa, abafundi bangaqonda ngokucacile izinzuzo nezimo zohlelo lokusebenza zohlobo lwe-cap \ septa, okwenza ukufezeka okukhulu nokunemba kwedatha ekuthuthukisweni kwemithi, ama-protemics kanye nokuhlolwa kwemvelo.
Ukuqalisa
Ku-chromatography ewuketshezi-mass spectrometry (LC-MS), ukulungiswa kwesampula okufanele kubalulekile ekutholeni imiphumela enembile, ethembekile. Le nqubo ikhishwa, ihlanza, futhi igxile ekuhlaziyweni okuhlosiwe kusuka kumatric ayinkimbinkimbi ngenkathi kuqeda ukungqubuzana kwekhwalithi yedatha yehlisa. Isampula yesampula ephezulu yekhwalithi yayo ithuthukisa ukuzwela nokucaciswa, okubalulekile emikhakheni efana nokuhlaziywa kwemithi, ama-protemics kanye nokuhlolwa kwemvelo. Imininingwane emincane efana nokuvalwa kwe-vial kungenza umehluko omkhulu: ukuvuza noma okungcona kusuka kuma-cap caps \ sepana azokubeka phansi isampula ngaphambi kokuthi kufike ku-LC-MS.
Iphoyinti eliyisihluthulelo: Sebenzisa ama-aijiren's CAPS ochwepheshe kanye ne-SEPTA ukuvikela ukungcoliswa kwesampula futhi kuqinisekiswe ubuqotho bemininingwane.
Ithuluzi le-LC-Of-art le-LC-MS elabhorethri, libonisa ukubaluleka kokulungiselela okwenziwe ngesampula.
1. Amasu ajwayelekile we-LCMS azolungiselela
| Isu lokwenza umsebenzi | Okuhlosiwe |
|---|---|
| Ukuzokulungwa | Isusa ama-Phalanisa (izihlungi ze-0.2 μm) ukuvikela amakholomu nama-nozzles. |
| I-Protein Perigitation | Sebenzisa i-solvents ye-organic (acetonitrile, methanol) ukusula amaprotheni avela kumasampula we-bio. |
| Ukukhishwa kwesigaba esiqinile (i-SPE) | Ukugxila kuhlaziya futhi kususe ukungqubuzana ngama-cartridgeges we-sorbent. |
| Ukukhishwa okuwuketshezi (i-Lle) | Ukudluliselwa kuhlaziywa esigabeni se-organic; eguquguqukayo kodwa ingahlehlisa. |
| Ukukhishwa okusekelwa uketshezi (bus) | I-automated Lle ku-inert isekela; kugwema i-emulsion futhi kusindisa izixazululo. |
| Ukusulwa | Amakhemikhali aguqula ama-analytes (e.g. methylation) ukwenza ngcono i-ionization. |
2. I-CAPS neSepta: Abaqaphi beqiniso lesampula
Ekuhambeni komsebenzi we-LC-MS, ukuvalwa kwe-vial kuyisithiyo sokugcina ngaphambi kokuhlaziywa. I-Septa ephezulu ne-CAPS iqinisekisa izimbumbulu zomoya, ukuvikela ukuhwamuka nokuvinjwa kwemvelo.
- I-PTFE \ silicone Septa: I-Menert yamakhemikhali, inciphisa izikhala.
- Izimbotshana ezihlanganisiwe: Septum zinamathele unomphela kwi-cap, ziqeda izingcezu ezixegayo kanye nokuqinisekisa iziMpawu ezingaguquki.
Inqubo efanelekile yokukhonkotha futhi ibalulekile: Qinisa ukuqina okungaphezulu kwe-Snugly kungaphazamisa i-Septa, ngaphansi kokuqina kubangela ukuvuza okuvuzayo. Isivalo esivikelekile, esifanayo sithuthukisa ukuvela kabusha futhi sivimbela ukungcoliswa kokuwela.
3. Ukuqhathanisa ama-cap \ septa kuzindlela zokulungiselela
| Amalungiselelo | Ukuvalwa okunconyiwe | Ngani? |
|---|---|---|
| Ukuzokulungwa | I-PTFE ejwayelekile | Ubuso be-inert bumelana nezixazululo; kuvimbela ama-carrover. |
| I-Protein Perigitation | Pre-slit ptfe \ abicone septum + screw cap | Ivumela ama-puncturer aphindaphindwayo ngaphandle kokususwa kwe-cap. |
| I-spe | Pre-slit ptfe \ abicone septum + screw cap | Ijubane elisheshayo umjovo; ugcina uphawu ngesikhathi sokugcina. |
| Lle \ sled | I-PTFE-Face Septum (PTFE \ Illicone Sandwich) + I-Bonded Cap | Iphikisa izixazululo ezinolaka; Idizayini ene-bonded ivimbela ukuguquka kwe-septum noma ukuvuvukala. |
| Ukusulwa | Bonded Ptfe \ abicone septum + aluminium cap | Inikeza uphawu lwesikhathi eside ngokumelene nokuhwamuka kanye nokufakwa komswakama. |
4
| Uhlobo lomkhiqizo | Into uqobo lwayo | Izici ezibalulekile | Izinja |
|---|---|---|---|
| Pre-slit ptfe \ abicone screw cap | Ptfe \ abicone septum + aluminium cap | Ukubhoboza okuphindaphindiwe; inert; Ama-extractable aphansi | I-LC-MS ephezulu kakhulu ye-LC-MS, ama-Autosamplers |
| Standard PTFE \ abicone screw cap | Ptfe \ abicone septum + aluminium cap | Uphawu olunenhloso ejwayelekile; ukuhambisana okubanzi kwe-solvent | I-Routine LC-MS VIALS |
| Isibopho seppum crimp cap | Al cap + isibopho septum | I-Septum inamathele unomphela; I-Airtitight Seal engaguquki | Izinhlelo ezizenzakalelayo, ukugcinwa isikhathi eside |
| Snap cap + irabha septum | Irabha septum + clamp yensimbi engagqwali | Izindleko eziphansi; Ingozi yeCoring | Ukuhlaziya okubucayi kwesabelomali, okungabucayi |
Ithebula: Ukuvalwa kwe-Aijiren Ukuvalwa & Ukuqhathanisa Umkhiqizo.
Ukugcina
Ukulungiswa kwesampula ye-LC-MS esebenzayo kuyisisekelo sedatha yokuhlaziya ethembekile. Kusetshenziswa ukuvalwa okulungile - okunjenge-PTFEEN's Pre-Slit PTFE \ Silicone Septa kanye ne-Bonded Caps - Kugcina Ubuqotho Besampula Ngokuvimbela Ukungcoliswa Nokuhwamuka. Ukuqhathanisa ukuvalwa kwendlela yakho kuqinisekisa imiphumela eqondile, evuselelekayo. Kwabahlaziyi beLab, amakhemikhali we-R & D, nama-labs afundwayo wesithathu, ukukhetha i-aijiren caps ephezulu ye-AIIIRET kanye ne-Septa kuxazulula amaphuzu ajwayelekile ezinhlungu (ukuvuza, imfucumfucu, ukulahleka kwesampula) futhi kusekela ukushiyeka kwesampula ye-LC-MS.
I-Key Takeaway: Izinga lekhwalithi ye-VIAL TAPS kanye ne-SEPATE zibalulekile ukuvikela ukulahleka kwesampula nokuqinisekiswa kokubumbana kwesiqinisekiso. Izixazululo zika-Aijiren zokubopha ziphakamisa ukusebenza kwe-LC-MS nokusebenza kwelebhu.
