Yikuphi i-buffer elungele isigaba sakho se-HPLC Mobile?
Ukukhetha i-buffer efanelekile yesigaba sakho se-HPLC Mobile kubalulekile ukuze kufinyelelwe ukwahlukana okuhle kanye nemiphumela ethembekile. Ama-Buffers adlala indima ebalulekile ekulawuleni i-PH, okuthinta ngokuqondile ukugcinwa nokuqedwa kokuhlaziywa kanye nokuhlaziywa kokuhlaziywa, ikakhulukazi lezo ezikhona. Nakhu okudingeka ucabangele lapho ukhetha i-buffer enhle kakhulu yohlelo lwakho lokusebenza.
1️⃣ Qonda ama-analemu akho
Ukukhetha kwe-buffer kuncike kakhulu ku-PKA yokuhlaziywa kwakho. Ngokufanelekile, i-PH yesigaba sakho seselula kufanele ibe ngaphakathi kwe-unit 1 ye-Pka ye-Buffer ukuqinisekisa amandla asebenzayo abubi. Isibonelo, uma umhlaziyi wakho ene-PKA ka-4,5, i-buffer ye-phosphate ene-PKA ezungeze leli nani afanelekile, ukuvumela ukuthi ulondoloze umhlaziyi we-Io State wawo oyifunayo.
2️⃣ Izinhlobo ze-buffer
Ama-Phosphate Buffers: asetshenziswa kabanzi ngenxa yebanga lawo elisebenzayo lokuphambuka (PH 2-8). Ziyahambisana nokuhlaziywa okuningi futhi zinikeze ukuqina okuhle.
I-Acetate Buffers: Ilungele izinhlelo zokusebenza eziphansi ze-PH, ngokuvamile zisuka ku-PH 3.6 kuye ku-5.6. Zisebenza ngokuhlaziywa kwe-acid.
I-Tris Buffers: evame ukusetshenziswa ezisetshenzisweni zebhayoloji, ikakhulukazi amaprotheni, kepha kungenzeka zingabi kahle ngokutholwa kwe-UV ngenxa yokungathathwa kwazo.
3️⃣ Cabanga ukufaneleka
Qinisekisa ukuthi i-buffer ekhethiwe iyahambisana nohlelo lwakho lwe-HPLC kanye nesigaba sokuhambisa ikholamu yakho. Amanye ama-buffers, njenge-citrate, angalungisa izingxenye zensimbi ezingagqwali ngokuhamba kwesikhathi, ngakho-ke cabanga ngemiphumela yesikhathi eside kwimishini yakho.
4️⃣ Monitor Buffer ukugxila
Ukuhlushwa okungu-25-50 mm kuvame ukusebenza ngempumelelo kuzinhlelo eziningi. Ukugxila okuphezulu kungathuthukisa ukwakheka okuphezulu nesinqumo kodwa kungahle kuholele ekutheni kwandiswe i-backpressure kanye nemvula engaba khona.
