Aug. 30, 2024
Yakakura Performance Liquid chromatography (HPLC) ndiyo kiyi yekuongorora maitiro inoshandiswa muminda muminda seyakasiyana sesayeti sesaiti, uye kuchengetedza kwezvakatipoteredza, uye kuchengetedza kwekudya. Mukati meiyo HPLC World, pane maviri main mapoka: Ongorora Hplc uye gadzirira HPLC. Nepo matekiniki ese ari maviri ashandisire nheyo dzakafanana kupatsanurwa makomputa, anoshandiswa nekuda kwezvinangwa zvakasiyana uye ane akasiyana ma paramita ekushandisa. Iyi blog ichatsvaga misiyano pakati pekuongororwa uye gadziriro HPLC, kusanganisira chinangwa chavo, chiyero chekushanda, zvishandiso, uye zvinoshandiswa.
Unoda kuziva kuzere inforti dzeHplc vials, ndapota tarisa chinyorwa ichi: Encyclopedia of HPLC vials
Kunzwisisa analytical HPLC
Tsanangudzo uye Chinangwa
Ongororo dzeHLC inotarisa kunyanya pakuparadzanisa uye kuzivisa makomputa mumuenzaniso. Chinangwa chekutanga ndechekuwana ruzivo uye huwandu hwehuwandu nezvezvinhu zviripo mumusanganiswa. Ongororo HRPLC inowanzo shandiswa:
Kuongorora Kwakakodzera: Kuzivisa zvikamu zve sample.
Kuongorora kwehuwandu
Chiyero chekushanda
Ongororo dzeHplc inoshanda pamwero madiki, kazhinji inosanganisira microliter samples majekiseni. Mavhoriyamu anoshandiswa kazhinji ari mu1 kusvika ku100 μl range, achibvumira mitezo yakawanda kuti uongororwe mune imwe kumhanya. Ichi chidiki chidiki chakanakira kuongororwa uye kudzora kwemhando.
Saizi saizi uye size
Iwo makoramu anoshandiswa mu ongororo kazhinji anowanzove zvidiki mune diameter, kazhinji akatenderedza 4.6 mm, uye akazara nediki stationary zvikamu (3-5 μM). Uku kurongwa kunobvumira kugadziriswa kwakanyanya uye kushanda kwakasimba kupatsanurwa, kwakakodzera kuongororwa kwakadzama.
Kuongorora Nzira
Ongororo dzeHplc inoshandisa nzira dzakasiyana siyana dzekuona, kusanganisira UV-inooneka, fluorescence, uye misa spectrometry. Sarudzo yekuona inoenderana nemhando yekuongororwa uye izvo zvakananga zvinodiwa zvekuongororwa. Vasiri-vanoparadza vanoongorora vanowanzoshandiswa kuona kuti sampuli inogona kuongororwa pasina kurasikirwa kwakasimba.
Unoda kuziva mhinduro makumi mashanu nezve HPLC vials, ndapota tarisa chinyorwa ichi: 50 Mibvunzo inowanzo bvunzwa paHPLC vials
Kunzwisisa Kugadzirira HPLC
Tsanangudzo uye Chinangwa
Kugadzirira HPLC, kune rumwe rutivi, zvinangwa kuti zviparadzanise uye kunatsa zvirevo zvakananga kubva musanganiswa. Chinangwa chikuru ndechekuzvitsaura huwandu hwakakwana hwechinangwa chakaenzana nekuwedzera kuongororwa kana kushandisa. Kugadzirira
HPLC inowanzo shandiswa kune inotevera inonyorera:
Yakasarudzika yekuzvisarudzira: kubviswa uye kucheneswa kweiyo chaiyo mishonga yekutsvaga kana maindasitiri ekushandisa.
Sampula Kugadzirira: Kugadzirira kwemasampuli ekuongorora kunotevera kana kuumbwa.
Chiyero chekushanda
Kugadzirira HPPLC kunoitwa pachiyero chakakura pane kuongororwa HPLC. Mifananidzo yemuenzaniso inogona kubva pamamiriyoni mashoma kusvika kune akati wandei marara, zvichienderana neyakatarisirwa kubereka kwechinangwa chakanangana. Chikero chakakura ichi chinobvumira zvakakwana huwandu hwezvinhu zvakacheneswa kuti zviunganidzwe kuti uwedzere kushandiswa.
Saizi saizi uye size
Iwo makoramu anoshandiswa gadzirira HPLC akakura zvakanyanya, kazhinji kubva pamakumi maviri emm kusvika zana mm kana yakakura mune diameter. Mbiru dzakarongedzwa neakakura stitial station phasiti zvikamu (kazhinji 10-20 μm) kuti uwedzere mareti uye ugare wakakura sampuli. Dhizaini iyi inobatsira kuderedza kudzvinyirira uye kuwana inoshanda kupatsanurana kwemakomputa.
Kuongorora Nzira
Mukugadzirira HPLC, nzira dzekuona dzinogona kusiyana neiyo dzinoshandiswa mukuongorora hplc. Nepo zvisiri-zvinoparadza zvichiratidzika, zvakarongeka zvinonyorwa zvinowanzoshandisa zvinoongororwa izvo zvinokwanisa kuunganidza zvidimbu zvichienderana neyekupedzisira profiles. Izvi zvinobvumira chaiyo componeunds kuti isatsoke seyavo elute kubva mukoramu.
Unoda kuziva zvakawanda nezve application ye chromatography vials, ndapota tarisa chinyorwa ichi:Nzira shanu dzekuchenesa dzeHPLC vials
Misoro yakakosha pakati pekuongororwa uye gadzirira hplc
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Chimiro
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Ongororo HPLC
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Kugadzirira HPLC
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Chinangwa
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Kuongorora uye kuwanda
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Kusarudzika uye kucheneswa kwemakomputa
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Chiyero chekushanda
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Diki-chiyero (μl mavhoriyamu)
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Yakakura-chiyero (ml to l volumes)
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Column Zvidimbu
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Diki DIAMETER (4.6 mm)
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Yakakura Diamita (20-100 mm)
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Size
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Zvidiki zvidiki (3-5 μM)
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Zvikuru zvidimbu (10-20 μm)
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Rates Rates
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Yakaderera inoyerera mitengo (0.15 ml \ / min)
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Yakakwira inoyerera mitengo (10-200 ml \ / min)
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Kuongorora Nzira
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Vasiri-vanoparadza vanoongorora (UV, MS)
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Kuunganidzwa Kwemubatanidzwa Kubva pane Elution Profil
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Kusarudza pakati pekuongororwa uye kugadzira HPLC
Kana uchisarudza pakati pekuongororwa uye gadziriro HPLC, funga zvinotevera zvinhu:
Chinangwa chekuongorora: Sarudza kuti chinangwa chikuru ndechekuti vave nehanya uye kuwanda kuongorora makomputa kana kuti zviparadzanise mishonga yakatarwa yekuwedzera kushandiswa.
Huwandu hwemuenzaniso: Ongorora huwandu hwemuenzaniso huripo. Kana huwandu hushoma hwemuenzaniso hwave kuwanikwa, imbiso HPLC inogona kuve yakakodzera. Zvekare, kana huwandu hukuru hwemuenzaniso hunodiwa, kugadzirira HPLL isarudzo iri nani.
Inodiwa kuchena uye goho: Funga nezve kuchena kunodiwa uye kuberekwa kwechinangwa chevanotarisana. Kugadzirira HPLC kunovavarira kuwedzera kupora kweiyo nzvimbo yakatarwa, nepo kuongororwa HPPLC kunotarisa pakupa ruzivo rwakadzama pamusoro pekuumbwa kwemuenzaniso.
Zviwanikwa nemidziyo: Ongorora inowanikwa zviwanikwa, kusanganisira zvishandiso uye bhajeti. Kugadzirira HPLC Systems inowanzo kuve inodhura uye inoda yakawanda yekudyara mumidziyo uye mari yekushandisa.
Inofarira Mastering HPLC Sampule Kugadzirira? Tarisa uone chinyorwa ichi:HPLC yemuenzaniso yekugadzirira mhinduro dzemhedzisiro mibairo.
Mhedziso
Ose Ongorora uye Ekugadzirira HPLC maitiro anokosha ari mumunda we chromatography, uye imwe neimwe inoshandiswa zvakasiyana zvichienderana nezvinodiwa nevaongorori uye indasitiri. Nepo Ongororo yeHPLC yakanakira kuongororwa uye kuwanda kwemakomputa, Kugadzirira HPLC kwakakosha kwekuparadzanisa uye kuchenesa makomputa chaiwo ekuwedzera maficha. Kunzwisisa misiyano pakati penzira idzi mbiri kuchabatsira vaongorori vanoita sarudzo dzine ruzivo, vodzokorora mashizha avo, uye vanowana zvakaringana uye vakavimbika mhedzisiro mukuongorora kwavo. Sezvo HPLC tekinoroji inoenderera mberi, ese eongororo uye nzira dzekugadzirira dzicharamba dziri chikamu chekubudirira kwesainzi yekutsvaga marudzi ese.
Chirungu
ChiChinese
