Lenane la chrotography ke mokhoa o matla oa tlhahlobo o lumellang karohano le tlhaiso ea likarolo tsa motho ka mong ka mohlaleng. Hangata e sebelisoa taolo ea boleng le lipatlisiso ka har'a k'hemik'hale, meshceotual le liindasteri tsa biotechnologight. Ho fana ka liphumano tse nepahetseng le tse tšepahalang, mohlala oa li-cumatographmon, karolo ea bohlokoa ea ts'ebetso ea ligramagraphy, e tlameha ho bolokoa e hloekile ebile e lokolohile litšila.

E na le phello e ntle ea ho hlatsuoa ka tlase ho masala a qhibilihileng le a masea a tsoang ho Sampulliok samlespole Vialis. Bohloeki bo kopana le litlhokahalo, mohato oa ho hloekisa o bonolo, 'me nako ea ho hloekisa e fokotsehile,' me ketso ea ho hloekisa e na le tikoloho e ntle haholo. Ka kopo khetha laboratori oa hau ho khetha ea hau!

Hajoale, ho na le tlhokomelo e fetang ea boleng le polokeho, theknoloji ea li-chromatographic e sebelisoa le ho tlhahlobo ea liteko tsa sehlahisoa, haholo-holo theknoloji ea li-chromatograph, e sebelisitsoe haholo.

Selemo se seng le se seng, palo e kholo ea lihlahisoa tsa temo (lihlahisoa tse ling tsa lik'hemik'hale, li-acid tsa manyolo Ka lebaka la palo e kholo ea mehlala, palo e kholo ea botlolo ea ente e hloka ho hloekisoa nako le nako, 'me ka linako tse ling ho na le litholoana tseo tlhahlobo ea litso li hloekisitsoengSampulliok samlespole Vialis.

Ho na le mekhoa e mehlano ea ho hloekisa Sampulliok samlespole Vialis:

Mokhoa oa 1:Ho beha tharollo ea ho hloekisa

1, Tlanya metsi mme o hlatsoe makhetlo a 'maloa.

2, beha e bema ka metsi a hloekileng, 'me ulrasound metsotso e 15.

3, Fetola metsi, ebe u hlola metsotso e 15.

4, e kolobile ka beaker ka metsi-etathan.

5, qetellong u tlohe moea oa tlhaho.

Mokhoa oa 2: Ho hloekisa o omileng

Ho sebelisa lesela la mahala la litlolo kapa ho hlakola, ho hloekisa ho omileng ke mokhoa oa ho hloekisa li-ciaromagraph tsa chromatography. Ho lahla litšila, hlatsoa li-vials ka lesela kapa u hlakola. Ebe li hlatsoa ka botlalo ka metsi a bolokiloeng 'me a ba tlohelle moea. Li-viovers tse sa sitoang haholo ke bakhethoa ba loketseng mokhoa ona.

Mokhoa oa 3: Ho sebelisa potasiamo sulfate potasiamo ea potasiamo ea potasiamo

1, ka kakaretso e nang le metsi a omileng metsi ebe o kolobisoa ka potasiamo ea sulfate potasiamo.

2, Sampulliok samlespole Vialis Mokhoa oa ho hlatsoa le ho etsa se tšoanang, o sebelisa joala ho feta lihora tse 4, ebe o sebelisa metsi ho fihlela hora, a itlhatsoa ka metsi kamora ho omella ho eona.

Mokhoa oa 4: Ho sebelisa methanol

1, qala ka methanol (Spicture e hloekileng ea botala) e kolobisitsoeng, 'me ho hloekisoa ha ultrasonic metsotso e 20, ebe methanol e omella.

2 Joale, tlatsa botlolo ea samromatographic e nang le metsi, ho hloekisoa ha ultrasoc ka metsotso e 20, ebe metsi a tla emisoa.

3, Kamora hore mehlala ea botlolo ea likhahla e omella.

Mokhoa oa 5: Ho sebelisa mokelikeli o matla oa oxida

1. Pele ka ho hloekisa metsi a matla a ho hloekisa metsi (chumate ea boima

2. Mme ka nako eo metsi a aparetsoeng ka nako ea ultrasonic a hloekisitsoeng ka makhetlo a mararo,

3. 'Me qetellong, ka ho hloekisa methanol hang, ho omisa ho ka sebelisoa.

Mekhoa e mehlano ea ho hloekisa ea Sampulliok samlespole Vialis li tsebisoa ho uena. Ke ts'epa hore o ka ela hloko bohloeki ba botlolo ea samromatographic.

Ho na le mekhoa e meng e meraro ea ho u khothaletsa:

Mokhoa oa 6: Ho hloekisa alkaline

Alkali Ho itlhatsoa ho kenyelletsa ho sebelisa tharollo ea Alkali, joalo ka hydroxide le metsi, ho hlatsoa li-culis tsa li-chromatography Tharollo ea alkali e lokela ho tšeloa ka sejaneng pele ho eketsoa. Kamora ho ipolaea ka lihora tse 'maloa, ba bo hlatsoa ka metsi a mabehileng, ebe ba ba omella.

Mokhoa oa 7: Ho sebelisa li-enzyme ho hloekisa

Ho hloekisa ka enzzyatic ho kenyelletsa ho sebelisa enzyme joalo ka amylase kapa tiesese ho hloekisa li-ciali tsa li-chromalete tsa cream. Kenya likotlo ka sejaneng ka mor'a ho eketsa tharollo ea enzyme ho eona. Kamora ho ipolaea ka lihora tse 'maloa, ba bo hlatsoa ka metsi a mabehileng, ebe ba ba omella.

Mokhoa oa 8:Ho hlatsoa khatello e phahameng

Ho hloekisaSampulliok samlespole VialisHa Washer ea leasher ea khatello e phahameng e tsejoa e le ho hlatsoa ha khatello e phahameng. Liphalletso li lokela ho kenngoa ka basketeng kapa ka terey ebe li kenya Persher khatello ea khatello e phahameng. Washere ona o hloekisa mashano a litšila tse neng li sebelisa jets ea khatello e phahameng ea metsi. Kamora ho hlatsoa, hlatsoa ka hloko li-vials ka metsing a jeoang ke metsi pele a li lumella hore li ome.

Ha re phethela:

Ho hloekisa mohlala o sebelisoang bakeng sa li-chlestography bakeng sa mohato oa bohlokoa ka mokhoa oa ho hlophisa boitokiso. Ho sebelisa mokhoa oa ho hloekisa o atlehang ho tlosa litšila leha e le life ha u ntse u itšireletsa khahlanong le tšenyo ea Val.

Seo u lokelang ho ela hloko:

Valia SeptaE tlameha ho nkeloa sebaka ke e ncha, haholo ha u sekaseka masale a likokonyana, o tlameha ho fetoha, ho seng joalo, e tla ama liphetho tsa bongata.
Haeba u hloka Valia Septa, ka kopo tlanya mona!

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E fetileng:

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E 'ngoe:

Liphate tsa li-chromatography tsa ciamatography li ka sebelisoa hape?