Mokhoa oa chrotography ke mokhoa oa tlhahlobo o sebelisitsoeng litabeng tse ngata tsa saense, ho tsoa ho k'hemistri le litlhare tsa tikoloho ho ea tikoloho ea tikoloho le tikoloho ea tikoloho. Ha o sebelisa karolo ea chromatography Empa laboratori o botsa hore na o sebelisoa hapeLi-vielis le ho koaloaho beha litholoana tsa tlhahlobo le ho nepahala ha data; Sehloohong sena re tla hlahloba lintho tse amang boitšoaro bona hammoho le mekhoa e metle bakeng sa ho sebetsana hantle.

Li-vielis le ho koaloa li-chromatography: Bohlokoa ba bona ba ho sekaseka

Litimale tsa ciaromagraphy le ho koaloa li sebetsa e le lijana tsa mantlha tsa disampole nakong ea ho hlahlojoa. Kahoo, ba tlameha ho latela litlhahiso tse tiileng bakeng sa ho sireletsa polokeho ea sampole, boloka bohloeki le ho hloekisa litšusumetso tsa kantle hore li se ke tsa kena-kenana le litholoana. Li-vials li tla ka lisebelisoa tse fapaneng joalo ka khalase kapa polasetiki ea inmert ho amohela mefuta e fapaneng ea mohlala le mahlakore a ho fetoloa, tšilafalo kapa ho ka ba le litlolo tsa bona.

Barekisi ba bangata ba k'hemik'hale le bafuputsi Botsa hore na Lithako tsa chromatography le ho koaloa e ka sebelisoa hape kamora ho hloekisa ka nepo ka Li-dissh tsa laboratoring(haholo-holo Bakeng sa ho hlatsoa li-glagware ea lab).

Hoetsasena bohlokoa Potso hlakisa, litsebi tse peli tsa United States le UK li ile tsa ithuta 'me tsa hlahisa litholoanaea ho sebelisa likotlo tse halikiloeng hape le ho koaloa Mo tsebeng ea mahlale 20670.

Fumana tataiso e felletseng ho lipotso tse 50 tse botsoa haholo mabapi le li-pilc vaialis tse boletsoeng sehloohong sena se ithutoang le se tebileng: Boholo ba 50 bo batloang khafetsa ho li-valc tse phehang

CHo lelekisoa ts'ebeliso ea ho hlatsoitsoe Lithako tsa chromatography le ho koaloa ka li-vaialis tse ncha li sebelisa khasecLiletsa tsa Hurnatografiels le tsa HPTC,Ba hlalosaMathata ka litšoantšo tse ngata le litafole,hape e entse Lihloela tse ngata tse ngata tsa li-viels tsa matsarase.RE khahla e khahla, TKe linepe tse sebelisoang hape tse bonts'ang likhahla tsa glue ho li-ke maqephe a tsoang matsaranka, le ho phalla ha khalase. Empa, tse khahlang haholo li ne li le mafeto a 100X a bonts'ang libaka tse otlolohileng tsa khalase tse hlakileng tsa khalase pele li sebelisoa le kamora ho hloekisa e sa tloaelehang e sebelisitsoeng.

Liqeto tse ling tse mabapi le likhathatso tsa ciaromatography le ho koaloa li kenyelletsa liphuputso tse latelang:

Likarolo tsohle tse ling li fumanoe ka likheo tsa MS ka har'a li-ms tse maemong ohle a liteko.

· Doownme bakeng sa sesebelisoa sa GC se ntse se eketseha ka lebaka la ho eketsa chelete e eketsehileng ea enjene ea lineer.

Li-entetion tse nyane li bonts'a kenyelletso ea litlhophiso tse eketsehileng ka mokhoa oa UV bakeng sa tlhahlobo ea seletsa sa Hplc.

Phefumoloho ea ente ea ente e tsoang ho li-vavers e ne e eketseha ka mokhoa o lekanyetsoang ha a sebelisa Sekundos e eketsehang ka jaro.

Ka lentsoe, likeletso tsa litsebi: "Litsenyehelo tsa nako tse sebelisoang ho lokisa lilaki le ho se sebetse lik'hemik'hale ho feta melemo ea ho e sebelisa Lithako tsa chromatography le ho koaloa. "

E thusa bohlokoa ba ho sebelisa li-vavers tse hlakileng tsa chromatography mme o utloisise karolo ea bona ea bohlokoa ho netefatsa sephetho se hlakileng: Hobaneng ha litemana tse hlakileng li khethoa bakeng sa chromatography?

Lintlha tse 4 tse susumetsang ho itlosa bolutu tsa vaes le ho koaloa

Lintlha tse ngata li bapala karolo ea bohlokoa ho khethoang hore na likhama tsa ciaroma ea li-chromatography li ka sebelisoa hape:

1. Bophelo ba boitsebiso

Khetho ea lisebelisoa tse Via le tse koalang li bapala karolo ea bohlokoa ho theosa le ho feta. Liphallelo tsa khalase, tse tsejoang ka hore li na le matla le ho hanyetsa ka lik'hemik'hale, hangata li bokelloa ho feta tse ling tsa polase; Lisebelisoa tsa ho koaloa ka har'a PTFE kapa silicone le tsona li fana ka ts'ebetso e ntlafalitsoeng ho feta ts'ebeliso e mengata.

2. Ts'ebetso ea ho hloekisa
Bakeng sa tšebeliso e atlehileng le ho koaloa, lits'ebetso tse atlehang tsa ho hloekisa li bohlokoa haholo. Ho tlosoa ka ho phethahetseng ha masala a fetileng a sampole ea nakong e fetileng le li-sinmints li tiisa hore ho sa tsotellehe lingoli, ha li ntse li netefalitsoe li-protocol tsa ho hloekisa li lokela ho hiroa bakeng sa liphetho tse khotsofatsang.

3. Mekhoa ea sterilization

Sterilization e bohlokoa ho qoba tšilafalo ea likhapha tse sebelisoang hape ho latela litlhoko tsa lik'hemik'hale le litaelo tse khethehileng ho tsoa lihloliseng tse khethehileng.

4. E loketse

Qetellong, ho ile ha sebelisoa likotlo le ho koalla le ho koaloa ho itšetleha haholo ka tšebeliso ea tsona. Le ha litlhahlobo tse itseng li ka emisa ka ho boloka liphetho tse nang le phello ea liphetho tsa liphetho tsa lipheo, likopo tse ling tse kenyelletsang lijana tse hloekileng kapa lithuto tsa bohlokoa li hloka liphetho tse hlakileng bakeng sa ho fana ka liphetho tse hlakileng.

Ebe bakeng sa tlhahlobo e ntle le e nepahetseng,Ho sebelisa bochaMalinyane a chromatography kapa ho koaloa ha se khetho e ntle, tlhoko leha e le efe eaLithako tsa Chromatograft, ho koalloa le lisebelisoa tse ling li ne li ikopanya le rona.

Bula likarabo ho tse 20 tse botsoang khafetsa ka li-valip tse kopantsoeng le hipc vaialis ka se ngotsoeng sena se felletseng le se rutang: Boholo ba 50 bo batloang khafetsa ho li-valc tse phehang

E fetileng:

Ka ho sebetsa hantle! Mekhoa e 5 ea ho hloekisa li-chromatography tsa mohlala oa mohlala

E 'ngoe:

Na u hlile u tseba li-chromatography tsa khatello tse phahameng tsa li-chromatography tse phahameng?