Umahluko phakathi kohlalutyo kunye nokulungiselela i-HPPCC
I-chromatography ephezulu ye-chromatography (i-HPPC) yindlela eyimfuneko kuhlalutyo lohlalutyo, esetyenziselwa ukwahlula, ukuchaza, kunye nezinto ezahlukeneyo kwiisampulu ezahlukeneyo. Nangona kunjalo, i-HPPC ingahlulwahlulwahlulwahlulwahlulwa kwiintlobo ezimbini eziphambili: Uhlalutyo lwe-hplc kunye ne-hplc efanelekileyo. Ukuqonda umahluko phakathi kwezi ndlela zimbini kubalulekile ukukhetha indlela efanelekileyo yesicelo sakho esithile.
I-1 ️⃣ i-hplc
Injongo: I-HPLC yohlalutyo ngokuyintloko igxile ekwenzeni nohlalutyo lobungakanani kunye nobungakanani beendawo ezihlanganisiweyo. Ijolise ekunikezeleni ulwazi oluneenkcukacha malunga nokuqulunqwa kwesampulu ngaphandle kokuzikhetha izinto.
Ubungakanani besampulu: Ngokwesiqhelo kubandakanya imiqulu emincinci yesampulu, rhoqo kuluhlu lweMicrolimatir. Iziphumo zibhekiswa kwiNkunkuma emva kokufunyanwa, njengoko injongo kukuhlalutya kunokuqokelela.
Ubungakanani bekholamu: Iikholamu zohlalutyo zihlala zincinci ubukhulu (malunga ne-4.6 mm) kwaye zipakishwe nge-partical yerenti (3-5 μm) yokufezekisa ukusonjululwa okuphezulu kunye neemvakalelo.
2️⃣ ilungiselelo lokulungiselela i-HPLC
Injongo: Ngokwahlukileyo, i-HPLC yokulungiselela yenzelwe ukwahlula kunye nokucoca izihlanganisi ezithile kumxube. Le ndlela ibalulekile ekufumaneni inani elikhulu lezinto ezisulungekileyo zophando okanye isicelo.
Ubungakanani besampulu: kubandakanya imiqulu emikhulu yesampulu, amaxesha amaninzi kwindawo yemililliter, kunye ne-EUEGE ejolise kubaqokeleli beqhinga lokuyahlulahlula.
Ubungakanani bekholamu: Iikholamu zokulungiselela zikhulu kububanzi (50-200 mm) kwaye zisebenzisa ubungakanani be-particle ye-pare (20-50 μm) ukuqhubela phambili amaxabiso aphezulu okutshintsha.