Ukuthengisa okushisayo kwekhwalithi ephezulu yokutakula i-HPLC
Ukukhanga okuyinhloko kwama-vials aphezulu aphezulu we-1.5 mL (30 μl) yivolumu encane. Lokhu kubenza balungele izinhlelo zokusebenza lapho kungenziwa khona ukuhlolwa kwevolumu emincane kuphela, njenge-bioanalysis, ama-proteomics, noma izinto ezizinzile ngokuphatha okukhawulelwe. Kodwa-ke, ivolumu elincane futhi lisho ukuthi noma imaphi amaphutha ngesikhathi sokuphatha angaba nomthelela omkhulu kumphumela wokugcina.
Lapho usebenzisa la ma-vials we-realents angu-micro-realent, kubalulekile ukuze uhlale ugcine ama-reagent vials endaweni efanele. I-30 μl reservoir itholakala ezansi kwe-reagent vial, futhi ukuhlolwa kungahle kube ngepayipi noma kufiswe kulesi sikhundla. Gwema ukulinganisa noma ukuhlela kabusha izipesheli ze-realent, ngoba lokhu kuzodala ukuthi ukuhlolwa kungene kuvolumu enkulu ye-1.5 ml, okwenza kube nzima kakhulu ukuthola ivolumu ephelele.
Ngaphezu kwalokho, qaphela lapho uphatha ama-reagent vials. Gwema ukuthuthumela noma ukunyakaza ngamandla, ngoba lokhu kungadala ama-bubble noma kudale i-realent yokulandela izindonga ze-reagent vial, okuholela ekulahlekelweni kwe-realent. Ngaphezu kwalokho, yiba mnene futhi uqinile lapho uxuba noma uguqula ama-reagents. Noma yikuphi ukunyakaza okungazelelwe noma okunobudlova kungaphazamisa izinga elibucayi le-reagent futhi kubangele ukulahleka okungalindelekile.
Izinto ezibonakalayo zengilazi zalezi zinhlaka zivame ukuhambisa futhi ziyahambisana nezinhlangothi eziningi ze-solvents kanye nokuhlaziywa. Kubalulekile ukuqinisekisa ukuhambisana kokuhlolwa okuthile nanoma yiziphi izithasiselo noma izithambisi. Amanye amakhompiyutha angahle ahlelele indawo yengilazi, okuholela ekubuyiselweni okuncishisiwe.
Cabanga ngezidingo zokuphepha zempumputhe lapho ugcina lezi zinhlaka ezihleliwe. Ezinye izakhi ezingazinzile noma ezibucayi zingadinga ukugcinwa emazingeni okushisa athile, njengesiqandisi noma ukuqanda, ukugwema ukonakala. Landela izimo zokugcina ezinconyiwe ukuqinisekisa ukunemba kwesikhathi eside kokuhlolwa. Isitoreji esingalungile, njengokuchayeka ekushiseni noma ekukhanyiseleni, kungahle kuholele ekushintsheni okungathandeki kokuhlola noma ukulahleka.
Nge-athikili kwisitoreji nokuphatha, sicela ubheke le ndatshana:Ungawugcina kanjani futhi uphathe ama-vials aphezulu we-1.5ml aphezulu aphezulu?