Sebakeng sa k'hemik'hale, chromatography ke mokhoa oa sekhukhuni o sebelisoang bakeng sa ho arola le ho sekaseka motsoako o rarahlang. Karolo e boima, karolo e mahlonoko ea ts'ebetso ea chromatographic, e sebetsa e le sejana sa sampole ho hlahlojoa. Mesebetsi ea kotlo, vaels e bile khetho e tloaelehileng ea likopo tsa lijalo tsa chutatograph tsa chromatography, ka lebaka la ho pepesetsoa pepenene le ho hloka toka. Leha ho le joalo, lilemong tsa morao tjena,Liakhisa tsa polasetiki tsa HelmatographyBa hlahile e le tlhokomelo e fapaneng, e khabisitsoeng ka melemo ea bona e ikhethang ho feta li-clues tsa khalase. Sehloohong sena, re kenella hare ho melemo e mene ea ho sebelisa li-grorams tsa polasetiki tsa chaboratoring.

1. Ts'ireletso e ntlafalitsoeng

Ho netefatsa tikoloho e bolokehileng ea laboratoring e bohlokoa haholo ho sireletsa bafuputsi le litsebi tsa lab tse tsoang likotsi tse ka bang teng. Ho pheha khalase ea khalase, leha e ne e sebelisoa ka mokhoa o sa tsotelleng ebile e kotsing ea ho khesoa. Kotsi ea khalase e felileng eseng e lebisang ho tahlehelo ea mehlala ea bohlokoa empa e boetse e baka kotsi ea likotsi. Taolo e nyonyehang, litabeng tsa polasetiki tsa li-chromama tsa li-chromatography li fana ka menyetla e fapaneng ho latela polokeho le ho letsa. Ho tsamaisoa ke lisebelisoa tse matla tse kang polypropingne kapa polyethylene, ho fokotsa monyetla oa ho arola ka laboratoring.

Ntle le moo,Li-vials tsa polasetikiba enjene ho mamella mefuta e mengata ea mocheso, e ba fe boikarabelo bo fapaneng ba chrotographic, ho kenyelletsa le likarohano tsa mocheso o phahameng. Ho hanyetsa ba lik'hemik'hale tse phahameng ho li sitisa ho senyeha, boloka botšepehi ba mohlala nakong ea polokelo le ho hlahlojoa. Ka litakatso tsa polasetiki tsa li-chromatography, bafuputsi ba ka tsepamisa likelello tse ngata mosebetsing oa bona ka kholiseho, ba tseba hore mehlala ea bona ea bohlokoa e ne e lula le sirelelitsoe.

Hlophisa tsebo ea ho itokisa haholo hoa charomatography ka mehato e 6 e bonolo bakeng sa tlhahlobo e nepahetseng le e tšepahalang. Bula liphiri joale! Mehato e 6 ho lokisa likhamati tsa chromiatography bakeng sa tlhahlobo

2. Khokahano ea lik'hemik'hale

Ho boloka bohloeki ba sampole ke karolo ea bohlokoa ea tlhahlobo e atlehileng ea chromographic. Liphaere tsa khalase, leha li sebelisoa hohle, li tsebisoa ho baka tšebelisano lipakeng tsa mohlala le boitsebiso bo boholo, bo lebisang ho nepahala ha sephetho sa tlhahlobo ea tlhaiso. Ka lehlakoreng le leng, likhama tsa polasetiki tsa li-chromatography li fumaneha ka lisebelisoa tse fapaneng, tse kang Polypropylene le Polyeth isathylene, e emelang lik'hemi tsa lik'hemik'hale tse ikhethang.

Ntho e ka bapisoang le lik'hemik'hale ea litsomi tsa polasetiki e thibela tšebeliso ea mehlala le boitsebiso bo boholo, kahoo ho boloka bo-mohlala, ho boloka mohlala oa mohlala mme ho bontša liphetho tse hlakileng. Karolo ena e ntle e etsa bafuputsi ho sebelisa mefuta e pharaletseng ea li-solvents ntle le likhathatso ka ho laetse kapa ho na le mathata. Ho hlophisoa ha lik'hemik'hale tse ngata tsa lik'hemi tsa polasetiki tsa chankana ea likheo tsa chankana le bafuputsi ba ho hlahloba lits'ebeletso tse thata.

3. TLHOKOMELISO EA METSOALLE

Mohlala oa ho adppippment ke ntho e bohlokoa ha e sebelisa li-viels tsa setso tsa khalase. Metsoako e meng, haholo-holo li-prosetis le liprotheine. Ho fapana le moo, likhama tsa polasetiki tsa chromagragraphy li na le sebaka sa ho rema semmuso.

Masene a ileng a fokola ka litsi tsa polasetiki a tiisa hore li-pokello ea sampole e se li fetohile, li hlahisa litholoana tse tšepahalang le tse sa fetoheng ea tlhahlobo e tšepahalang le tse sa fetoheng. Tšobotsi ena e ntle ke ea bohlokoa ka ho fetisisa ha e sebetsa ka mantsoe a manyane a mohlala kapa delpole ea bohlokoa, moo esita le tahlehelo e fokolang e ka ama haholo tšusumetso ea tlhahlobo ea tlhahlobo. Ka ho fokotsa ho rema mohlala oa mohlala,Li-vavers tsa polasetiki tsa polasetikiFana ka bafuputsi ba ntlafalitsoeng kelellong le ho tšepahala ho lipatlisiso tsa bona tsa chromaticgraphic.

Mehlamiti ea bohato bo felletseng ba 16 ho hloekisa li-racks tsa li-hplc tsa Hplc tse sa tsebeng letho Ma-Delec Mehato e leqepheng la 16

4. Sebetsa se theko e tlase

Boemong bofe kapa bofe ba laboratori, theko ho ne ho etsa qeto ea ho etsa liqeto tse ho etsa liqeto. Litabeng tsa polasetiki tsa chakolo ea polasetiki li hlahisa tharollo ea litšenyehelo tse theko e boima ha e bapisoa le balekane ba bona ba khalase. Mokhoa ona oa ho etsa lipolanete oa polasetiki ka kakaretso o sebetsa hantle hape o sa sebetse hantle, o tlatsetsa litšenyehelo tsa bona tse tlase. Ka lebaka leo, li-laboratories li ka fumana masela a maholo a polasetiki bakeng sa tekanyetso e tšoanang, e li etsa mesebetsi e metle bakeng sa merero ea lipatlisiso tse nyane le tse kholo.

Ho feta moo, pheko ea lintho tse litšila tsa polasetiki e fetolela bophelong bo bolelele ba sehatsetsing, ho khothaletsa likotlo tse ngata ntle le ho tlohela botšepehi ba bona ba sebopeho. Sena se atolosa bophelo bo botle ba Viales, o lebisang ho tse fokolang mme o kenya letsoho bakeng sa chelete e ngata ha nako e ntse e tsamaea. Motsoako oa ts'ebetso ea theko e boima le khetho e nang le molumo oa li-chromani tsa polasetiki bakeng sa li-laboratori tse batlang ho ntlafatsa ho nepahala ho nepahala.

Sephetho

Ka kakaretso, melemo ea ho sebelisa liliba tsa li-choratotograph tsa polasetiki tsa li-choratoto li le bohlokoa ebile li na le malinyane. Lintho tsena li khothaletsa polokeho ea ho ntlafatsoa, ho ba le mokhoa o kholisang ka lik'hemik'hale, leha mohlomong o fokotsa kotsi ea ho rema mohlala oa mohlala le ho fana ka tharollo ea litšenyehelo tse theko e boima. Ha tšimo ea k'hemistri ea sekaseka e ntse e tsoela pele e fetoha,Li-valc tsa polasetiki tsa HPLCBa hlahile e le khetho e khethiloeng bakeng sa bafuputsi le bo-rasaense ba batlang mohlala o tla ba oa ho qala nakong ea ho hlahloba chromatograk. Ho khahlisa tsoelo-pele ena theknoloji ea vaerano ho ka ntlafatsa tšebetso ea bona ea li-chromatograph, 'me u ntlafatse ho nepahala ha data, u lebisang ho tse ncha tsa ho sebelisa bohloeki ba saense.

Lekola tataiso ea mantlha ho li-pilti tsa HPLC, tse arabang 50 hangata tse botsoang khafetsa sehloohong sena se felletseng se ngotsoeng.Boholo ba 50 bo batloang khafetsa ho li-valc tse phehang

E fetileng:

Ke mang ea sebelisang lipitsa tsa likhahla tsa chromatography?

E 'ngoe:

Litlhahlobo tsa boleng ba Phatlalatso ea Litoro tsa Chromatography