I-LC-MS vs GC-MS: Yiliphi inqubo engcono kakhulu ocwaningweni lwakho?
Uma kukhulunywa ngokuhlaziya izingxube eziyinkimbinkimbi nokukhomba amakhompiyutha angaziwa, abacwaningi bavame ukuphenduka baphendukela kuma-chromatography-mass spectrometry (LC-MS) noma i-Chromatography (GC-MS). Womabili la makhono womabili anikela ngamakhono anamandla okuhlukaniswa kanye nokutholwa, kepha ukukhetha phakathi kwabo kuya ngezidingo ezithile zocwaningo lwakho. Ake sihlole ukwahluka okusemqoka futhi sikusize ekuboneni ukuthi iyiphi indlela elungele kangcono izidingo zakho.
1️⃣ Ukuvumelana okuvumelana
I-LC-MS iguquguqukayo ngokwengeziwe, njengoba ingaphatha uhla olubanzi lwamakhompiyutha e-polar ne-non-polar, kufaka phakathi lezo ezi-therrhally noma ezinama-molecular izinsimbi eziphakeme. Lokhu kwenza kube nengqondo yokuhlaziya ama-biomolekyuli, amakhambi emithi kanye nokungcola kwemvelo.
I-GC-MS, ngakolunye uhlangothi, ilungele kangcono ukuhlaziya amakhompiyutha ajwayelekile anobuthakathaka kanye ne-semi-turatile. Kudinga amasampula abe manzi aqine futhi akwazi ukuhlukaniswa ngaphandle kokubola.
2️⃣ ukuzwela nokukhetha
Womabili la masu anikela ngokuzwela okuphezulu nokukhetha, kepha amanani athile ancike kwindlela ye-ionization esetshenzisiwe. I-ElectrosPray Ionization (ESI) e-LC-MS ne-Electron Ionization (EI) ku-GC-MS ivame ukuqashwa.
I-GC-MS Ngokuvamile ihlinzeka ngokukhetha okungcono ngenxa yamandla aphezulu okuxazulula i-chromatography yegesi, kanti ama-LC-MS angafinyelela umkhawulo wokutholwa okuphansi kwezinye izinhlanganisela.
3️⃣ Ukulungiswa kwesampula
Ukulungiswa kwesampula kwe-LC-MS ngokuvamile kulula, njengoba isampula ingachithwa kwi-solvent efanelekile futhi ifakwe ngokuqondile ohlelweni. I-GC-MS ivame ukudinga izinyathelo ezingeziwe zokulungiselela isampula, njengokususelwa ekufuneni, ukuthuthukisa ukuguquguquka nokuqina okushisayo.
4️⃣ Isikhathi sokuhlaziya
I-GC-MS ngokuvamile ihlinzeka ngezikhathi zokuhlaziywa ngokushesha uma kuqhathaniswa ne-LC-MS, okwenza kube lula ukuthola izinhlelo zokusebenza eziphezulu. Kodwa-ke, isikhathi esiphelele sokuhlaziya futhi sincike ekubumbeni kwesampula kanye nokwahlukana okudingekayo.
Ukugcina
Lapho ukhetha phakathi kwe-LC-MS ne-GC-MS yokucwaninga kwakho, cabanga ngohlobo lwamasampula akho, ukuhlaziya okuhlosiwe, kanye nokuzwela okudingekayo nokukhetha. Uma amasampula akho ayizinzile futhi aguqukayo, i-GC-MS ingaba ukukhetha okungcono. Ukuze uhlaziye amakhompiyutha we-themally armially noma aphezulu, ama-LC-MS kungenzeka ukuthi yindlela efanelekile. Xhumana nochwepheshe emkhakheni wakho futhi ubheke izidingo ezithile zephrojekthi yakho yocwaningo ukwenza isinqumo esinolwazi.
