Abin da ba a tantance abubuwan da ba a cikin ba ne ta GC-MS?

Gas din Chromatography-Mass Spectometry (GC-Ms) wani tsari ne mai mahimmanci wanda aka yi amfani da shi sosai don bincika mahadi da ƙwayoyin cuta. Koyaya, ana iya amfani dashi don bincika mahaɗan da ba avolatile ta hanyoyi daban-daban, gami da dernivatization. Wannan labarin yana binciken nau'ikan mahaɗan marasa ma'ana ta GC-Ms, mahimmancin su, da hanyoyin da ake amfani da su don gano su.

Kuna son ƙarin sani game da banbanci tsakanin LC-MS da GC-Ms, don Allah a duba wannan labarin:Menene banbanci tsakanin LC-MS da GC-MS?

Menene mahaɗan marasa amfani?

Abubuwan da ba a jera ba abubuwa ne waɗanda basa amfani da sauƙi a zazzabi a ɗakin. Sun kasance gaba ɗaya mafi girma kwayoyin ƙwayoyin nauyi da polarity, suna ba su ƙasa da nazarin kai tsaye ta hanyar GC-Ms ba tare da gyara ba. Misalai gama gari sun hada da:

Polymers da ƙari: abubuwa da aka yi amfani da su a cikin robobi da kayan marufi.

Biomolecules: kamar amino acid, sunadarai, da wasu lipids.

Manabbobin magunguna: Aiwatar da kayan masarufi (apis) da metabolites ɗinsu.

Picarancin muhalli: mai tawali'u gurɓataccen ƙwayar cuta (pops) da ƙarfe masu nauyi.

Dabarun lalata

Don bincika mahaɗan da ba ta dace ba ta amfani da GC-MS, sau da yawa ana buƙatar dervatization. Wannan tsari ya shafi gyara fili don ƙara haɓakar haɓakarsa ko kwanciyar hankali. Hanyoyi na gama gari sun hada da:

Silanization: Sauya aiki hydrogen atoms a cikin rukuni na aiki tare da rukuni na silicon (E.G., trimethylsl). Wannan hanyar tana da tasiri ga giya, Amines, da carboxylic acid.

Acylation: Wannan hanyar tana gabatar da kungiyoyin ACYL don haɓaka haɓakawa kuma ana yawanci amfani da su don mai kitse na mai da amino acid.

Methyn shaida: Wannan dabarar tana ƙara ƙungiyoyi methyl zuwa mahadi don ƙara yawan volatility da ganowa.

Wadannan dabarun lalata na iya canza abubuwan da ba a canza su ba cikin tsari wanda GC-Ms zai iya bincika shi ta hanyar GC-Ms da yadda ya kamata.

Don ƙarin bayani game da Autosampler Vials don gas Chromatography, koma zuwa wannan labarin:2 ml autosampler vials don gas chromatography

Menene mahadi marasa ma'ana da ba a cikin ba za'a iya amfani da GC-ms don bincika?

1.

An yi amfani da GC-MS sosai don nazarin abubuwa masu haɗari marasa ƙarfi da aka lissafa ta hanyar hukumomin muhalli. Misali, Amurka kare muhalli kariya (EPA) ta gabatar da hanyoyin nazarin fifiko kamar:

BIPHYHLANCELUSLS (kwastomomi): Anslicarancin masana'antu da aka sani don dagewa matsayin sa.

Magungunan rigakafi: sharan gona daga ayyukan gona waɗanda ke gurbata ƙasa da ruwa.

Iyakar ganewa saboda waɗannan mahadi yawanci suna tsakanin 1 zuwa 28 PPB, suna nuna babban abubuwan jinsi na GC-Ms lokacin da aka haɗa tare da dabarun hakar da suka dace kamar Micme).

2. Binciken aminci na abinci

A cikin yankin abinci na abinci, ana amfani da GC-ms don gano ɓoyayyen ɓoyayyen mara kyau wanda zai iya ƙaura daga kayan tattara kayan cikin abinci. Wadannan crass sun hada da:

Filin da aka kara: sunadarai sun kara wa robobi don kara sassauci; Misalai sun hada da kalmar sirri.

Don ƙari: Misali, antioxidants ko abubuwan da aka adana waɗanda zasu iya zuwa abinci.

Thearfin nazarin waɗannan mahadi yana da mahimmanci don tabbatar da amincin masu amfani da ka'idojin tsarin.

3. Hanyoyin magunguna

Binciken magunguna sau da yawa yana buƙatar gano nau'ikan kayan masarufi marasa ma'ana da metabolites ɗinsu. Misalai sun hada da:

Sinar da magunguna masu aiki (API): Ofishiniyar samar da kayan aikin da ke da alhakin sakamako na warkewa.

Metabolites: kayayyakin da aka kirkira yayin metabolism na magani a cikin tsarin nazarin halittu.

GC-Ms yana ba da damar cikakken bincike game da waɗannan mahadi, suna taimakawa a cikin tarihin magunguna da haɓaka ƙwayoyin cuta.

4. Samfurori na halitta

A cikin metabolomics, GC-ms ana amfani dashi don bincika abubuwan da ba a cikin ba a cikin samfuran ilimin halittu kamar fitsari ko jini. An yi nazari akai-akai sun hada da:

Amino acid: Ginin shinge na sunadarai, wanda zai iya nuna matsayin abinci mai gina jiki ko cuta na rayuwa.

Organic acid: metabolites da ke da hannu a cikin hanyoyi daban-daban na bitchemical.

Wannan aikace-aikacen yana da mahimmanci don fahimtar sa hannu na metabolic a cikin mahallin kiwon lafiya da cuta.

Hanyoyin GC-MS na tantancewa

Samfura Samfura

A lokacin da nazarin mahaɗan da ba mai canzawa ta amfani da GC-ms, ingantaccen samfurin yana da mahimmanci. Dabaru na iya haɗawa da:

Hakar ruwa-ruwa (lle): daban-daban na daban daga matririces matrices.

Mai ƙarfi lokaci na hakar (AYaki): Iya mai da hankali gaji daga hadaddun gaurawar kafin bincike.

Kayan aiki

Saitin saitin GC-ms ya hada da:

Gas Chomatograph: Raba kayan haɗin da aka samo asali bisa rarrabuwar kawunansu tsakanin tsararraki da na wayar salula.

Mass spectrometer: Gano mahadi dangane da taro-da-caja rabo (m \ / z), samar da bayanan tsari.

Bincike na bayanai

Da zarar an sami bakan da yawa, wanda aka bincika bayanai ya shafi tattara taro zuwa wani ɗakin karatu ko cibiyar bayanai don gano mahaɗan gano mahadi. Kayan aikin kayan aikin software na gaba yana sauƙaƙe wannan kwatancen, don haka yana haɓaka ganewa.

Shin ka san bambanci tsakanin HPLC VIals da GC Vials? Duba wannan labarin:Menene banbanci tsakanin vials vials da GC Vials?

Ƙarshe

Gas din Chromatography-Mass Spectometry ya kasance mafi mahimmancin fasaha a cikin masu ilmin sunadarai na rashin iya faruwa, da amincin abinci, da metabolomics. Yayinda nazarin kai tsaye na waɗannan mahadi suna ƙalubalan saboda kayan aikinsu na asali, dabarun lalata sun fadada ikon aikace-aikacen GC-MS. Kamar yadda hanyoyin nazarin ci gaba da samo asali, GC-ms zai iya buga muhimmin mahimmanci wajen tabbatar da aminci da kuma yarda da ci gaba a cikin binciken kimiyya.