Dauko kayan m vials shine maɓallin key. Yin amfani da su sosai, ma. Suna ƙayyade yadda amintacce ne da cikakken bincike na HPLC shine. Versionarin girman mutum na iya tasirin samfurin ingancin. Hakanan yana shafar daidaito na bayanan. Wannan labarin yana bincika dabarun don inganta haɓakar HPLC Autosamppler Volume. Manufar shine a yanke asarar samfurin kuma ci gaba da bincike cikakke.

Fahimtar abu ɗaya

Akwai daidaitattun girma na HPLC na HPLC na HPLC autosampler Vials, kamar 1.5-2, 4 ml, da 150-300 μL micro abun ciki. Mafi yawan amfani da shi da iya biyan bukatun gwajin yau da kullun sune vials ml 1.5-2. Lokacin da samfuran buƙata mafi girma fiye da 3 ml, muna amfani da mafi girma 4 ml. Wadannan manyan vials na iya riƙe fayil ɗin da ke cikin su. Ana amfani da waɗannan don ƙanana samfurin samfurin, tsakanin 150 da 300 μL.

Sanin halaye da kuma amfani da amfani da waɗannan masu girman kai na HPLC autosampler vial masu girma dabam shine maɓalli. Zabi girman madaidaiciya mai mahimmanci yana da mahimmanci. Yakamata ya dace da girman samfurin. Wannan yana taimaka rage asarar samfurin kuma ci gaba da samfurin lokacin bincike.

Kuna son ƙarin sani game da abubuwan haɗin HPLC, don Allah a duba wannan labarin HPLC vealss: Inganta daidai da samfurin mutuntaka

Yadda za a zabi bisa ga samfurin

Zabi girman vial da ya dace yana da mahimmanci don rage asarar samfurin. A matsayinka na gaba ɗaya mulkin, ƙara samfurin ya kamata ya dace da ikon vial. Amfani da vial wanda ya yi yawa sosai don samfurin na iya ƙirƙirar kai da yawa. Wannan yana kara hadarin fitar ruwa, musamman ga nazarin masu canzawa. Tattaunawa, zabar vial wanda yake maɗaukaki na iya haifar da samfurin overflow da asara.

1.Shsess da samfurin girma:

Aunawa ko kimanta ƙarar samfurin HPLC.

Yi la'akari da canje-canje na da ake buƙata. Wadannan canje-canje na iya zama saboda dilution, sake sarrafawa, ko sauran matakan shirye-shiryen.

2.Match da samfurin girma zuwa ga masu girma dabam na gama gari:

Don kananan Samfuran Samfura (150-300 μL), shigar da shigar micro a cikin babban vial (E.G., 2 ml) ana bada shawarar.

Don matsakaiciyar samfurin (0.5-2 ml), daidaitaccen 1.5-2 ml vials yawanci shine mafi kyawun zaɓi.

Don mafi girman samfurin sample (har zuwa 3 ml), 4 ml vials na iya zama mafi dacewa.

3.CONIDSPACE:

Nufin rage girman kai (girma mara komai) a cikin Vial.

Headspace wuce gona da iri na iya ƙara haɗarin ƙwararrun samfurin samfurin, musamman don nazarin masu canzawa.

Yanke girma ya kamata ya kasance a kusa da lambar septum ko hula don ƙirƙirar hatimi mai sauƙi.

4.Alasan bukatar da aka saka:

Don kankanin samfurin, micro saka a cikin babban vial zai iya rage kai tsaye. Wannan saitin ya kuma rage girman bayyanar samfurin.

Micro mai saka hannu sun zo a cikin kundin daban-daban, kamar 150-300 μL. Zasu iya dacewa da masu girma dabam dabam.

Kuna son sanin yadda ake zaɓar nau'in nau'ikan micro-saka don cratomatography vials Yadda za a zabi nau'in hannun da ya dace don vials din chromatography vials

5.Test ka tabbatar:

Gudanar da gwaje-gwajen farko ta amfani da girman vial da aka zaɓa da duk wani abu.

Saka idanu da samfurin murmurewa, kwanciyar hankali, da kuma duk alamun rashin ruwa ko lalata.

Daidaita girman vial. Ko, yi amfani da abubuwan da ake buƙata. Wannan zai inganta tsarin samfurin da kuma adanawa.

Bi wadannan matakan. Kuna iya samun girman HPLC na HPLC don girman samfurin ku. Hakanan zai taimaka wajen rage asarar samfurin. Ka tuna yin la'akari da dukkan samfurin kayan aikin gaba ɗaya. Hakanan, la'akari da kowane bincike na musamman yana buƙatar lokacin da ka zaba.

Kuna son sanin yadda za a zabi hula mai kyau don vial din ku na chracomatography vial, duba wannan labarin Yadda za a zabi nau'in da ya dace don vials ɗinku

Rage kai tsaye da bayyanawa

Abu mafi mahimmanci shine zai iya zaɓar ƙarar vial da ya fi dacewa da yawan samfurori. Wannan yana taimaka rage rage kan headspace kuma ya rage yankin yanki wanda aka fallasa ga yanayin kewaye. Ta hanyar daidaita girman vial zuwa ƙarar samfurin, zaku iya rage asarar asara saboda lalacewa ko ɓarna.

Idan samfurin ya zama ƙanana da mafi girma vial, da babu yawa ko wofi girma za a ƙirƙira a cikin Vial, wanda zai hanzarta samfurin rashin ruwa da sulhu samfurin. Don rage wannan haɗarin, mafi ƙarancin vial girman da zai iya ɗaukar ƙarar samfurin yayin da har yanzu ana buƙatar sa ido da kulawa da kulawa. Tabbatar da cewa samfurin yana cikin hulɗa kai tsaye tare da mai septum ko hula yana taimakawa ƙirƙirar hatimin ƙasa kuma yana rage fallasa yanki.

Leverging vins

Abubuwan da ke cikin vial, musamman Micro Insits, na iya samar da ƙarin Layer na kariya don karamin Samfuran Samfura. Wadannan abubuwan shigarwar da suka dace a cikin manyan masu girma dabam, yadda ya kamata, rage haɓakar kai da rage girman bayyanar. Misali, zaku iya tabbatar da amincin ƙananan ƙananan samfurin samfurin ta saka abubuwan da aka shigar a cikin Vials ml.

Ƙarshe

Zabi wanda ya dace HPLC Autosampler vigaid yana da matukar mahimmanci don kiyaye amincin bincike na bincike na samfurin ku. A cikin wannan labarin, mun koya game da kundin vial gama gari, yadda za a zabi m vial, kuma me yasa suke da mahimmanci. Zamu zabi vial vial don rage asarar samfurin kuma samar da abin dogara data.

Binciken aikace-aikacen Chratomatography vials a cikin abinci da kuma abin sha na gwaji, bincika mahimmancin matsayin muhimmiyar don tabbatar da aminci da ƙimar ƙa'idodi:HPLC samfurin shirye-shiryen shirye-shirye don kyakkyawan sakamako

A baya:

Shin kun taɓa san farashin HPLC Vials?

Mahimmancin hadin gwiwar HPLC Vials don cikakken bincike na samfurin