HPLC samfurin prreatment - jagorar mataki-mataki-mataki

Sample prreatment

Sample pratestment ya hada da dukkan ayyukan kafin allura. Ban da yin nauyi, narkewa, da dilution, kuma ya ƙunshi matakai masu zuwa:

• Tanki
• Hakar
• Drivatization (pre-shafi derumn)
• Liquidography na ruwa (mai matsin lamba na matsin lamba)

Ana iya yin tsinkaye da hannu ko kuma sarrafa kansa. Shin ka san darajan samfurin samfuri?

Deseratization

Wasu samfures har yanzu ba za a iya allurai kai tsaye bayan sun faɗi ba. Ana buƙatar derivatization don yin ƙwayoyin cuta ba tare da ɗaukar hoto ba ko kyankyasawa ta hanyar UV ko masu ganowa masu kyalli.

• Inganta hankali
• Inganta rabuwa da (canji mai inganci)

Abubuwa masu yiwuwa sun haɗa da asarar samfurin, gurbatawa, basu dace ba, ko samfuran da yawa, wanda zai iya shafar daidaito ko kuma gabatar da kurakurai.

Sample Glatration

Samfuran LC dole ne su zama uniform da barbashi-free; barbashi na iya lalata na'urar da aka yi ko toshe shafi. Matakai sun hada da:

• Shake samfurin kafin allura kuma bincika barbashi, turbi, ko emulsions
• Idan an lura da barbashi, tace samfurin amfani da membrane wanda zai iya riƙe barbashi ≥0.15μm barbashi ≥0.15μm barbashi

M maganganu: gurbataccen samfurin samarwa, gurbataccen samarwa, rage haɓakawa mai narkewa, da kuma sauran ƙarfi ɓawo a haifar da kurakurai.

Hanyoyin hakar

Hadawa ya raba sunadarai daga haɗin haɗin kai tsaye ko kuma yana cire abubuwa masu hadewar (misali, sunadarai). Abubuwan da ke tattare da kwayoyin cuta yakamata su kasance mai ƙarancin guba, maras tabbas, ƙasa da ƙazanta, kuma narke samfurin.

Hanyoyi na yau da kullun: difyl oneher, ethyl acetate, Dichloromethane, Chiloroflororm, Benzene, ko gaurayawan.

Bayan hakar, samfurori za a iya allura kai tsaye ko mai da hankali ga ƙara nazarin taro da hankali.

Hakar ruwa mai narkewa

1. Abubuwan haɗin acidic da salts: ire-iren impurities tare da abubuwan kwayar halitta, daidaitawa da acidic, sannan cirewa ko a cire; ko bushe a karkashin n₂ kuma narke a cikin abubuwan da ya dace kafin allura.
2. Abubuwan da aka gyara na asali da salts: cirewa impurities tare da abubuwan kwayar halitta, daidaita zuwa asali, sannan cirewa ko allurar; ko bushe a karkashin n₂ kuma narke a cikin abubuwan da ya dace kafin allura.
3. Abubuwan tsawaitarwa na tsaka tsaki: cire rashin ingancin kwayar halitta, sannan na bincika kai tsaye ta hanyar juyawa-lokaci juyawa-lokaci juyawa-lokaci juyawa-lokaci juyawa-lokaci juyawa-lokaci juyawa na chromatography.

Hakar mai mai narkewa

Cirewa tare da abubuwan da ke tattare da cuta ko kuma bajectory kai tsaye; ko bushe a karkashin n₂ kuma narke a cikin abubuwan da ya dace kafin allura.

Mazaunin shiga

Yawan wadatattun hanyoyin gurbata a cikin bincike:

Tsarin Tsarin bayani

• Shirya misali mafita na ciki da farko; Yi amfani da tsari iri ɗaya cikin ƙa'idodi da samfurori don rage kurakurai
• Abubuwa dole ne su zama chromatography-aji da barga
• Adana daidaitattun hanyoyin sadarwa a cikin kwalaben launin ruwan kasa a ƙarancin zafin jiki
• Don bitamin, amfani da launin ruwan kasa mai launin shuɗi ko kare daga haske

Matsaloli na yau da kullun a cikin protreatment da mafita