vials-adsoriking vials, vial surface jiyya, silanized vials, peg mai rufi vials, pfdcs shafi

A cikin manyan-sarkin da aka yi nazarin, asarar adsorption akan saman shimfidar vial na iya iyakance daidaitawar ganowa. Groupsungiyoyin Silanol (Si-Oh) da kuma gano rashin ƙarfe na ƙarfe a cikin gilashin hydrogen tare da kwayoyin cuta, impar polar ko compled polar da aka caje a jikin bango. Borosiated borosilate vials sau da yawa yawan amfanin miyagun ƙwayoyi ko magungunan biomolecule da ke ƙasa da 80%, da kuma samar da kayan aiki na atomatik suna fama da mummunan yanayin zancensu. Millelors suna ba da shawarar siilanized gilashin vials don nazarin alamomin PLAR, da kuma nazarin sun nuna har ma samfuran PPB sun rasa sigina a cikin gilashin da ba latsawa ba. Sabili da haka, pastarwar farfajiya ko shafi yana da mahimmanci don daidaitaccen matakin matakin daidaito.

2. Gilashin wuraren aiki da hanyoyin adsorption

a. Kungiyoyin Silanol da Ions Karfe
  i. Kungiyoyin surface Si-Oh tare da aka yi wa annan nazarin
  II. Gano karfe ions kafa alamomin lantarki tare da cajin kwayoyin

b. M girgiza
  i. Abubuwan da Kwayoyin cuta (E.G., ACN, Meha) na iya lalata yadudduka, bayyanar da sabon shafuka

c. M gurbatawa
  i. Resultual Commed ko kwayoyin halitta a kan bango suna haifar da koguna na fatalwa a cikin biyun

d. Tasirin tsarin sarrafa kansa
  i. Maimaita allura a cikin manyan tsarin ingancin karuwa na polar ko kuma tantancewa
  II. An bayar da rahoton asarar siginar sau da yawa ya wuce 10% akan lokaci

3. Ka'idojin jiyya na farfajiya: Kashewa Vs. shafi

3.1 Kayayyakin gargajiya

a. High-zafin jiki yana sauri (~ 800 ° C)
  i. Tsabtace wasu Si-Oh amma ya bar ions na karfe m

b. Acid Wanke (E.G., 6 m HCL)
  i. Yana cire ions karfe amma gilashin roughens farfajiya

c. A wanke (E.G., 1 m noh)
  i. Yana haifar da ƙarin sites si-o⁻, mai haifar da matsala

d. Iyakance
  i. Kawai wani sashi bangare ne na wuraren aiki a kan substrate

3.2 Silanization

a. Jiyya na kwayoyin halitta a karkashin matattara
  i. Orgases
  II. Haifar da katangar ruwa mai hydrophobic wanda ya tsuda zafi, acids, da tushe
  III. Lowers a saman tashin hankali da kuma mayar da mai binciken Polar zuwa sama da 90%

b. Misalan masu siyarwa
  i. "DV" silanized vials ga bincike na polar-foction (Ruwa)

3.3 Aiki mai aiki

a. Edluordecyltrichlortrihlorosilasi
  i. Monolayerded Monollayer yana ba da isasshen surface
  II. Mafi dacewa ga PAHs da LIPID-SLURBLANCT

b. Polyethylene glycol (peg)
  i. Hydrophilic Chainins ya ji sunadarai, Peptiges, da kuma tantance ruwa mai narkewa
  II. Yana ba da kariya mafi girma ga biomolecules

4. Ikon Shafin AdSorption da bayanai

a. Tasirin pastivation
  i. Silane yadudduka Silender Grand Graphobobic, Tarewa Polar daurin Polar
  II. Tsawaita bayan nutsarwa a cikin ACN ko Meoh

b. Dawo da aikin
  i. Silanized vials Kula da kusa da-100% dawo da 1 ppb Dexepin akan lokaci
  II. Val-da aka rufe vials cimma nasarar 97-99% na Polar सAR-Lactams sama da 72 H
  III. PFDCs vials ya wuce 90% dawo da Pahs Idan aka kwatanta da ƙananan dabi'u akan gilashi

c. Matsalar Adsorption
  i. An tantance Polar: PEG> Silanized ≈ PFDCS> Kashe
  II. Rashin daidaitawa: PFDCS> Silancized> Kashe> Peg

5. Zabin aikace-aikace da mafi kyawun ayyuka

a. Yi daidai da magani ga samfurin sunadarai
  i. Kwayoyi Polar, sunadarai, sunadarai, carbohydrates): Yi amfani da silanized ko peg gashi
  II. Ordungiyoyin da ba a Nasara (PAHs, Lipophilic gubobi): Yi amfani da sutturar pfdcs
  III. Samu samfurori: Silanization yana ba da daidaitawa

b. Yi la'akari da sauran ƙarfi da muhalli
  i. Silane Solein ya yi haƙuri da ph 1-12 da yawancin kwayoyin halitta
  II. Polymer mayu na iya karkatar da ƙasa a ƙarƙashin maye gurbin oxidiz ko babban zafi; la'akari da ptfe da aka saka ko volypropylene vials don matsanancin yanayi

c. Yankunan samfurin da Tsarin allura
  i. Don microvolumes (<100 μL) ko maimaita samfuri, yi amfani da sutura mai dorewa
  II. Kula da Haɗin Haɗin kai tsaye Ta Faɗin Taya (> Mats 10 ° Matsarancin Garuwar Rashin Rashin Gano) Kuma Blank Res (Seloxane Gudun A M \ / Z 207, 281)

d. Kasafin kuɗi
  i. Kashewa: Mafi tsada farashi, dace da koyarwa ko kuma hotunan hoto
  II. Silanized vials: Kudin tsakiyar kewayon, babban HPLC \ / LC-MS Aikace-aikace
  III. PEG \ / PFDCS Cooks: Farmakiyar farashi, dacewa da mahimmancin civiollyes da kuma gano gwajin muhalli

6. Kammalawa: daga jirgin sama mai zuwa ga mai aiki

Kamar yadda bincike na bincike ya kai PPB \ / PTPT matakan, samfurin vials ya zama mai amfani da aiki a maimakon kwantena masu amfani. An yi niyya da low-adsorving jasin da suka sauya asara cikin sigogin da ke sarrafawa. Zaɓin Vial da Jiki na samaniya sune mahimman abubuwan cikin ƙima kaɗan. Ta hanyar daidaitawa da ke tattare da fasahar sunadarai, dakunan sunadarai, juya vials cikin kayan aikin takamaiman, inganta inganta daidaito da haifuwa a cikin binciken binciken.

Mahimmin aiki

1. Don nazarin bincike mai zurfi, amfani da vasssivated vials

2. Dakatar da POLARIRT: Silanized \ / PEG don Hydrophilic, PFDCs don Hydrophobic

3. Saka idanu: Ci gaba da tsabta, ɗakunan lamba kusoshi, gudanar da blanks, maye gurbin rashin nasara

4. Balance Cost vs. Halin bayanai: Premium Smalling Rage Risuns da Kwarewa na karya