Tufafin shirye-shiryen samfurin gama gari don GC-ms

Gas din Chromatography-Mass Spectrometry (GC-Ms) dabara ce mai ƙarfi wanda aka yi amfani da shi don bincika m mahadi da ƙwayoyin setivolatile. Ya danganta da yanayin samfurin da masu bincike na bincike, ana iya amfani da dabarun dabaru da yawa don shirya samfurin yadda ya kamata samfurin. Wadannan fasahohi na yau da kullun suna amfani da samfuran donBinciken GC-MS:

Kuna son ƙarin sani game da banbanci tsakanin LC-MS da GC-Ms, don Allah a duba wannan labarin:Menene banbanci tsakanin LC-MS da GC-MS?

1. Ruwa samfurin shiri

Ana yin dilutuka na ruwa guda na ruwa a cikin ƙaramin tafasasshen ruwa kamar methanol, acetone, ko Dichloromethane don cimma nasarar kimanin 0.1 zuwa 1 mg \ / ml. Wannan yana tabbatar da cewa samfurin ya dace da tsarin GC kuma yana rage haɗarin cleting ɗin.

Filurtawa: Kafin bincike, ya kamata a tace samfurin don cire duk wani barbashi waɗanda zasu iya tsayar da bincike. A0.22 μM Filteryawanci ana amfani dashi.

Centrifugation: Don samfurori waɗanda zasu iya ƙunsar da daskararru, centrifugation na iya taimakawa raba ruwa daga kowane abu da aka ƙididdige da canja wurin zuwa Vial.

2. M samfurin shiri

Rikita: Dole ne a narkar da samfurori masu ƙarfi a cikin ƙarancin tafasasshen ruwa. Addara karamin adadin ('yan hatsi) na m ga m na vial na sauti da sau da yawa don tabbatar da cikakkiyar rushewa.

Drivatization: Ga Semi-maras kyau ko Pullatile na Polar, Deerivatization na iya zama dole don haɓaka haɓakar haɓakawa da haɓaka tunanin ganowa. Wannan ya shafi inganta masu bincike don sanya shi ƙarin amenable zuwa binciken GC.

3. Binciken HeadSpace

Headspace na Static: A wannan hanyar, an rufe shi da aka rufe da shi a cikin zafin jiki a akai zazzabi don yaduwa cikin kai tsaye samfurin sama da samfurin. Da zarar ma'auni ya kai, ana iya samin wannan headspace don bincike ta amfani da sirinji mai girman gas.

Hernamic Headpace (purge da tarko): Wannan dabarar ta ƙunshi iskar gas a cikin samfurin don haɓaka hakar kayan masarufi a cikin kai. Wannan hanyar tana ƙaruwa da hankali ta hanyar mai da hankali mai narkewa kafin bincike.

Kuna son ƙarin sani game da dalilin da yasa ake amfani da hotunan kai na kai a cikin cututtukan cututtukan cututtukan fata? Me yasa ake amfani da vials kanun headspace a cikin cututtukan cututtukan fata? 12 Angles

4. Fasahohin hakar

Sosai Matsi na MIXTraction (Spme): Spme yana amfani da zaren zare tare da tsarin hakar don ɗaukar mai bincike daga cikin ruwa ko tsarin gas. Wannan dabarar tana ba da damar yin samammen kai tsaye ba tare da bukatar karuwa ba kuma yana da matukar amfani ga mahadi mahadi.

Haɗin ruwa-ruwa (lle) da kuma tsayayyen lokaci (LE): Waɗannan hanyoyin ana amfani dasu don tsabtace samfuran shiga cikin matsalolin da ke tattare da abubuwan haɗin kai a gabanin GC-MS.

5. Tips don mai da hankali

Ana amfani da wannan dabarar don mai da hankali ga samfuran ƙwayoyin cuta a ƙarƙashin rafin nitrogen, wanda ke taimaka wajan yin ƙididdigewa.

Sample shiri la'akari

Tabbatar cewa duk abubuwan da aka yi amfani da su suna da olatatila kuma sun dace da GC-ms; ruwa da rashin ƙarfi da ba su guji ba.

Samfurori dole ne su ƙunshi kowane ɗumbin acid, bots, salts, ko wasu ƙyallen da zasu iya lalata shafin GC na GC ko tsoma baki tare da bincike.

Samfurori na ƙarshe ya kamata ya kasance kyauta na barbashi kuma ana iya shirya shi a cikiGilashin vials don hana lakabin kayan daga filastik.

Don ƙarin bayani game da Autosampler Vials don gas Chromatography, koma zuwa wannan labarin: 2 ml autosampler vials don gas chromatography

Ƙarshe

Ingantattun samfuran shirye-shiryen samfuri suna da mahimmanci don binciken GC-MS. Kowace hanya tana da fa'idodi kuma tana da takamaiman aikace-aikace dangane da yanayin samfurin da kuma manufa ta. Ta hanyar amfani da waɗannan dabaru, manazarta na iya haɓaka daidaito, hankali, da haifuwa na bayanan da suka dogara da filaye daban-daban kamar su.