Shin kun san game da ƙwayar ƙwayar cuta?

Takardar Chromatography dabaru ne wanda aka yi amfani da shi wanda ke raba shi da gano ga ga abubuwa daban-daban dangane da wani tsararru na tsararru (takarda) da kuma sashen hannu (da sauri). Yana da amfani musamman a cikin ilmin sunadarai da nazarin halittu don nazarin ɗimbin abubuwa masu yawa kamar alamu, amino acid, da sauran mahadi na kwayoyin.

Menene takarda microomatography?

Takarda Chromatography wani nau'in gurbi ne mai ruwa wanda matakin farko shine takardar tace takarda da kuma lambar lokaci shine sauran ƙarfi wanda ke gudana ta hanyar takarda tace. Hukumar ta dogara da bambancin mahaɗan tsakanin tsararren lokaci (takarda talla) da kuma sangare). Kamar yadda sauran hanyoyin motsa saman takarda ta hanyar daukar mataki, sai ya ɗauki abubuwan da aka cakuda cakuda a matsayinsu daban-daban saboda matsugensu biyu.

Ana iya fahimtar ka'idodin bayan da aka gano takarda ta hanyar manyan abubuwan biyu: bangare na chromography.

Bangaren Chromography: A cikin wannan nau'in, abubuwa ana rarraba su tsakanin matakai biyu na ruwa. A tsaye daga matakin ya ƙunshi ruwa a cikin pores na takarda tace, yayin da Madau shine sauran ƙarfi wanda ke motsawa cikin waɗannan pores. Rackaractaka na faruwa ne saboda mahadi daban-daban suna da alƙarya daban-daban don tsararrun tsitattun abubuwa.

Adsorption Churchption: Anan, m farfajiya na takarda yana aiki azaman mai tsayayye kuma ruwa mai ƙarfi yana aiki azaman matakin hannu. An gyara kayan a cikin cakuda dangane da adsorption zuwa m farfajiya da kuma siyarwarsu a cikin ruwa.

Abubuwan haɗin

Takalma mai tsayi: takarda chromatographic, yawanci ana yin shi da selulose. Yana bayar da farfajiya don abubuwan da zasu bi.

Mayoshi: Masu ƙarfi ko cakuda abubuwan sha da ke motsawa a kan takarda ta hanyar ɗaukar mataki. Zabi na sauran ƙarfi na iya shafar tsarin rabuwa.

Samfura: cakuda da za a bincika, yawanci an hango shi a wani farkon farawa akan takarda.

Kuna son ƙarin sani game da shirye-shiryen samfurin HPLC, don Allah a duba wannan labarin: HPLC samfurin shirye-shiryen shirye-shirye don kyakkyawan sakamako

Tsarin micramography tsari

Shiri: Zaɓi takarda tace tace tare da wani pamorosalli don tabbatar da ingantaccen rabuwa. Aiwatar da karamin sashi na samfurin cakuda zuwa tushe na takarda chromatography takarda.

Samfura Samfura: narke samfurin cakuda a cikin abubuwan da suka dace a zaman wani ɓangare na wayar hannu.

Spotting: Yi amfani da prillary ko micropipette don sauke karamin adadin samfurin samfurin akan jigon a kan takarda tace.

Haɓaka: tsoma bakin takarda na gwajin a cikin haɓakar haɓakawa (Nan gaba). A matsayina na printry mataki yana faruwa, da maxvent ya hau ta hanyar gwajin takarda, dauke da samfuran samfurin tare da shi.

Rarraba: Kamar yadda sauran hanyoyin motsawa, abubuwan da aka tsara daban-daban na samfurin za su motsa a ƙimarsu daban-daban saboda ma'amala daban-daban.

Abun gani: Bayan sauran ƙarfi gaba ya koma nesa nesa, an cire takarda daga sauran ƙarfi kuma an bar shi ya bushe. Za'a iya ganin abubuwan da aka kera su ta amfani da UV, ko kuma na karɓar sunadarai, ko ta hanyar kallon canje-canjen launi.

Bincike: Distance tafiya da kowane bangon dangi zuwa ga sauran ƙarfi dangi da aka auna don ƙididdige darajar RF (riƙewa) don tantancewa.

Aikace-aikace na takarda

Takarda Churomatognography yana da kewayon aikace-aikace da yawa a fannoni daban-daban:

Biochemistry: Binciko na amino acid, sugars, da sauran biomolecules.

Man Pharmaceuticals: Share na kayan aiki masu aiki da impurities a cikin tsarin miyagun ƙwayoyi. Amfani da shi don gano mahadi masu aiki da impurities a cikin tsarin magani yayin ci gaba da kulawa mai inganci.

Ana amfani da kimiyyar muhalli: An yi amfani da Chromatography don gano gurbata cikin samfuran ruwa da nazarin gurbatawar ƙasa a cikin ƙasa.

Masana'antar Abinci: Kwarewar tana taimakawa wajen yin nazari kan ƙari, gurbata, da danko don abinci don tabbatar da bin ka'idodin aminci.

Abvantbuwan amfãni na kamshi

Ingantaccen Kudin: Yana buƙatar kayan aiki kaɗan da kayan idan aka kwatanta da sauran dabaru na chromatographic kamar HPLC (babban aiki ruwa na ƙwayar cuta). Sauki: Tsarin yana da sauƙin sauƙin gaske kuma ana iya yin shi da kayan aikin ɗakunan ajiya na asali, don haka za'a iya amfani dashi don dalilai na ilimi.

Bukatar muryar ƙara: Ana buƙatar ɗan ƙaramin samfurin ne don bincike, yana sa ya dace da ƙimar samfuri.

Askar: zai iya raba abubuwa da yawa dangane da kaddarorin sunadarai, gami da polar da ba zato ba tsammani

Iyakantarwa na takarda chromatography

Duk da fa'idodinsa, takaddun kwamfutocin cututtukan fata yana da wasu iyakoki:

Iyaka mai iyaka: gabaɗaya ya dace da ƙaramin adadin samfurin.

Rediriti: ƙudurin ƙila ba zai zama babba kamar yadda manyan dabaru da aka samu kamar su HPLC ko gas ba (GC).

Lokaci yana cinyewa: Saboda ƙarancin ƙaura na ruwa, tsari na iya ɗaukar lokaci mai tsawo idan aka kwatanta da sauran hanyoyin chromatographann.

Karatun adadi na adadi: Yayinda za'a iya samun sakamako mai sauƙi, ana iya buƙatar ƙarin hanyoyin da za'a iya amfani da ƙarin hanyoyin calibration na iya buƙatar taƙaita taro.

Takarda Chromatography ya kasance wata dabara mai mahimmanci don rabuwa da kuma nazarin kamawa a fannoni daban-daban. Sauƙi, tasiri-da-tasiri, da ikon samar da sakamako mai zurfi game da kayan aiki mai mahimmanci don masu kimantawa masu kimantawa da masu binciken. Duk da iyakokinta idan aka kwatanta da manyan dabarun chromatographic, har yanzu ana amfani dashi sosai don dalilai na ilimi da kuma bincike na farko a cikin dakunan gwaje-gwaje.

Kuna son sanin amsoshin 50 game da vials HPLC, don Allah a duba wannan labarin: 50 Mafi yawan tambayoyi akai-akai akan HPLC VIALS