Samfura Samfura & Flimration: Tabbatar da inganci a cikin Chromatography

Abubuwan da suka dace na Samfura na Samfura, Cire nazarin, Enrich Doke, kuma, in da ake buƙata, kawar da tsaftacewa da matsin lamba, tabbatar da karama, tabbatar da karama. Gazawa a nan yana haifar da tsantsa hargitsi, ko kuma katangar shafi.

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Al'amari

• Baselon Drift da kuma tashin amo Unscure karamin kololuwa.

• Kololuwa da ba a tsammani sun bayyana daga rashin haƙuri.

• Rashin daidaituwa tsakanin allura.
  

  

Dalili

• Residims a cikin gilashin gilashi: Ba a cika shi ba wanke-wanke-wanke-wanke-wanke; Filastik na iya maganin filastik.

• Masu tsabtace jami'ai: Gangarin kwayoyin a cikin kurkukun da aka rage.

• Kamfanin muhalli na muhalli: ƙura na iska da kuma muryar iska Adsorb a buɗe samfuran bude samfuri.
  

  

Bayani

1. Tsaftacewa mai tsaftacewa: Acid jiƙa → Ultrasonic Rufe ruwa mai tsarkakakken ruwa → nitrogen bushe don gilashin gilashi; sadaukar da kayan maye don robobi.

2. Pre-filtration: Centrifuge a 3000rpm na 10min ko amfani da prefilter 5μm kafin kilomita na karshe 0.22μm.

3. Ma'ana mai sarrafawa: Yi tsari a cikin hoodarfin-flowed hood don rage karamar iska.
   
   

2. Tace clogging daga m tace

Al'amari

• Autosampler matsa lamba ararms da kuma sauyawa allura akai-akai.

• Rage raguwar kwarara, ganyen yanki.

• Daukaka mai tallafawa da shafin intanet na Column.
  

  

Dalili

• Membrane mismatch: nailan swees a cikin kwayoyin halitta; Pvdf creeps a cikin zafin jiki.

• Girman Pore Girma: 0.22μm tarket tarko mai kyau batsa amma rage kwarara; 0.45μm matakai na iya har yanzu clog tare da microcrystals.

Bayani

1. Membrane-Subvent Matrix: Yi amfani da pes: ac / mce don mai ruwa ruwa (ph4-8), ptfe hyfe \ / pvdf don matakan kwayoyin halitta; Guji cin ganyayyaki a cikin acid mai ƙarfi.

2. Zaɓi Girman Pore Mai dacewa: Yi amfani da 0.45μm don yau da kullun HPLC, 0.22μm na Uhplc ko aiki na yau da kullun; Samfuran Prefilter mai girma-Sanarwa ta hanyar 5μm.

3. Saka idanu Δp: Bibiya hauhawar matsin lamba; Sauya tacewa lokacin da mai ba da tallafi yana ƙaruwa> 15%.
   
   

   

3. Masu amfani-membrai na kwayar cuta

Al'amari

• Samfuran sikelint ɗin ya bayyana girgije ko yana ɗaukar barbashi.

• Sabuwar hayaniya ta Baseline ko fatalwa ta posting posting.

• Rage dawo da dawowa.
  

  

Dalili

• Subvent kumbura: thf kumbura PTFE, canza girman pore da gudana.

• Membrane leadarina: nailan a cikin tsari a cikin acid saki nilomomers da ke sha kashi 214nm.

Bayani

1. Gwajin dacewa: Jiƙa tacewa a cikin abubuwan da aka shirya don 24h a 25 ° C; Yi bincike don kumburi ko canjin nauyi.

2. Matashin Pilot LC-MS: Kwatanta tote ion chomomatogram na tace samfurin vs. m samfurin vs.

3. Yi amfani da manyan leach matattara: Ultrasonic-welded ptfe ko peres ba tare da adhereves ba.
   
   

Tsarin inganci: Gyaran Kulawa mai Kulawa

Tsaron gida

Daidaita Sanda don Tsabtace, Filmtration, da zaɓin membrane don hana kurakurai.

Saka idanu

Rikodin rarar, da baya, da kuma blank-samfurin buplines don gano drift.

Gyara

Kula da kuskure na tantanin Itace na Rapid-dalili da kuma gyara matakin.

"Tsarin nazarin yana farawa da hadawa da mafi ƙarancin barbashi."