Zabi na hannun dama na HPLC Mobile Passi yana da mahimmanci don cimma iyakar rabuwa da bincike game da ku samfurori. Bufers yana taimakawa wajen kula da ph, wanda yake da mahimmanci don sarrafa Iliazzation yanayin nazarin ku, don haka yana shafar lokacin riƙewa da siffar ganawa. A ƙasa akwai cikakken bayani game da yadda za a zabi mafi kyawun buffer don aikace-aikacen HPLC.

Shin kun san game da shirye-shiryen samfurin HPLC? Latsa wannan labarin don ƙarin koyo: "Duk abin da kuke buƙatar sani game da shirye-shiryen samfurin HPLC"

Muhimmancin zaɓin buffer

Buffer shine mafita wanda pH bai canza ba bayan ƙara karamin adadin acid ko tushe. Dangane da abun da suke ciki, za a iya rarrabe su kamar yadda acidic ko na asali. Tasirin da aka ƙaddara shi ta hanyar Pka, wanda ya fi dacewa ya kasance tsakanin ± 1 1 pH ya zama naúrar Mobile PH don ingantacciyar ikon tarko.

Mahimman dalilai don la'akari

1.ph. Wannan yana taimaka wajan kawar da acidic ko na asali, yana iyakance riƙe su akan shafi. Misali, idan mai bincike shi ne rauni a ɗan acid tare da PA na 4.5, ta amfani da Pay En da Ph kusan 2.0 zai ci gaba da kasancewa a cikin jihar da ba ta haihuwa ba.

2. Nau'in nau'ikan: buffers na kowa da aka yi amfani da shi a HPLC sun haɗa da:

Phasphate buffers: Inganci a cikin kewayon 2.0 zuwa 8.0, ana amfani da buppast na phosphate sosai sakamakon kwanciyar hankali da jituwa da yawa. Suna da dacewa sosai ga aikace-aikacen LC-UV.

ACETate buffers: Waɗannan suma sun shahara ga LC-UV Assays kuma ana iya amfani dashi a cikin wannan ph jeri kamar yadda phosphate buffers.

A cikin aikace-aikacen LC-MS, masu zuwa suna kamar tsari, Acetate, da Bicarbonate an fi son su a lokacin aiwatar da tsarin spectromy.

Baby maida hankali: Na saba fara maida hankali ga buffers a HPLC yana tsakanin 25-50 mm. Mafi girman maida hankali na iya inganta sifar ganɗuwa da inganci amma yana iya haifar da hazo lokacin da aka haɗe da haɗe da abubuwan da ke tattare da abubuwan sha. A bu mai kyau a ci gaba da maida hankali a kasa 25 mm don kauce wa rikice-rikice.

4. Nmiwical tsarkakakkiyar: Koyaushe amfani da babban-fina-finai na Chromatographic-sa buffers don hana gurbatawa da tabbatar da sakamako mai aminci. Rashin daidaituwa na iya haifar da ma'amala da ba tsammani da bambanci a cikin lokutan riƙewa.

Tasirin zazzabi: zazzabi na iya tasiri akan PH na mafita na mafita. Yana da mahimmanci a shirya da adana buffers a zazzabi mai sauƙi don kula da ingancinsu.

Kuna son sanin amsoshin 50 game da vials HPLC, don Allah a duba wannan labarin: 50 Mafi yawan tambayoyi akai-akai akan HPLC VIALS

Nasihu masu amfani don shirye-shiryen buffer

Shirin shiri: Za'a iya shirya abubuwa da yawa ta hanyar haɗawa da kayan haɗin acidic da na asali ko kuma mai taken bayani mai mahimmanci tare da acid. Guji daidaitawa da pH bayan ƙara abubuwan da aka gina kwayoyin cuta, saboda wannan na iya haifar da rashin daidaituwa sakamakon.

Sabbin hanyoyin: Shirya mafita mai amfani akai-akai don rage girman haɓakar ƙwayoyin cuta, wanda zai iya shafar aikin Chromatographic.

Guji lalata: wasu buffers, irin su citrate bakin karfe na iya cinye kayan karfe na HPLC. Tabbatar da cikakkiyar ƙwayar tsarin bayan amfani da irin wannan buppers don hana lalacewa.

Ƙarshe

Zabi mai da ya dace don wasan kwaikwayon ka na HPLC yana da matukar muhimmanci ga cimma sakamakon da aka amince da shi. Yi la'akari da ph, nau'in buffer, taro, da tsabta yayin zaɓar. Ta bin wannan, za ku iya inganta hanyar HPLC ɗinku don raba ta yadda ya kamata kuma ta bincika samfuran ku.

Kuna son sanin farashin HPLC VIals, don Allah a duba wannan labarin: Farashin HPLC Vials: 50 mafi yawan tambaya tambaya tambaya

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