A cikin babban mahimmin binciken bincike mai zurfi (ppb\/ ppt matakan), muna damuwa akan sinadarai na ginshiƙai da ƙimar ƙayyadaddun ƙima, duk da haka sau da yawa muna yin watsi da farkon saman samfurin mu ya taɓa: bangon vial. Ga duk wanda ke tafiyar da UHPLC-MS\/MS a cikin proteomics ko toxicology na asibiti, daidaitaccen gilashin gilashin ba akwati ba ne kawai ba - tarkon sinadarai ne wanda zai iya lalata sakamakonku kafin allurar farko.

Wataƙila kun ga alamun: raguwar 20% a cikin kololuwar yanki kawai saboda samfurin ya zauna a cikin autosampler na dare, ko hauka "kololuwar fatalwa" wanda bai kamata ya wanzu ba. Yawancin lokaci, mai laifi ba kayan aikinku bane; shine silanol kinetics a saman gilashin ku.

1. Ciwon kai na Adsorption: Me yasa Polar Analytes "Bace"

直观展示碱浸出。普通玻璃（左）会导致样品碱性漂移，而 Aijiren 硅烷化瓶（左）会导致样品碱性漂移，而 Aijiren 硅烷化瓶（烷化瓶！

Standard borosilicate gilashin, ko da high-karshenUSP Type 1 Glass, yayi nisa da rashin aiki. Tsarin masana'anta yana barin saman rarrafe tare da ƙungiyoyin Silanol masu amsawa (Si-OH).

Tarkon musanya na Ion: A pH> 3, silanols suna ɓarna kuma sun zama mummunan caji. Idan kuna aiki tare da magunguna na asali ko peptides, za su tsaya a bangon gilashin da wuya ba za su taɓa isa ga mai gano ku ba.
Kololuwar Tailing: Ko da a ƙananan pH, haɗin gwiwar hydrogen tsakanin gilashin da samfurin ku yana haifar da tsorokololuwar wutsiya. Ba ku ganin ainihin taro; kana ganin abin da ya rage bayan vial "ya yanke shi."

2. Tushen-Hanyar Haɓaka: Haƙiƙan Rushewar Haƙiƙa

Manta da arha "ruwan tsoma" vials. Rufe ruwa sau da yawa yana "puddles" a kasan vial - daidai inda 'yan ƙananan microliters na samfurin ku ke zama.

直观展示碱浸出。普通玻璃（左）会导致样品碱性漂移，而 Aijiren 硅烷化瓶（左）会导致样品碱性漂移，而 Aijiren 硅烷化瓶（烷化瓶！

A Aijiren, muna amfani da Jigilar Vapor Deposition (CVD). Mun buga gilashin tare da iskar organosilane a cikin injin daskarewa a babban yanayin zafi.

The "Invisibility Cloak": Wannan tsari yana haifar da haɗin gwiwa na dindindin, gina garkuwar jiki wanda zai hana samfurin ku "gani" gilashin gilashin mai amsawa.
Sifili Bleed: Wannan shine dalilin da ya sa ƙwararru ke nacewaSilanized HPLC Vials-Hanya ce kawai don tabbatar da samfurin da kuka shirya shine samfurin da kuke allura.

3. Real-World Data: LC-MS\/MS farfadowa da na'ura

A cikin bincike-bincike, rabon Sigina-to-Noise (S\/N) shine komai. Ƙananan gwangwani ba kawai cin siginar ku ba; suna zubar da ions wanda ke lalata tushen ku.

直观展示碱浸出。普通玻璃（左）会导致样品碱性漂移，而 Aijiren 硅烷化瓶（左）会导致样品碱性漂移，而 Aijiren 硅烷化瓶（烷化瓶！

Analyte (10 pb)	Std Borosilicate farfadowa da na'ura	Ajiren Silanized farfadowa da na'ura	Ingantawa
Magungunan Ƙarfi Mai Ƙarfi	68.4%	99.2%	+30.8%
Peptides na roba	45.1%	97.8%	+52.7%
PFAS (PFOA)	74.3%	98.5%	+24.2%

Lab Pro Tukwici: AmfaniLC-MS\/MS don gwajin ƙwayoyi? Silanization shine mafi kyawun kariyarku daga sodium (Na+) adducts wanda ke rikitar da siginar ku kuma yana danne siginar ku.

4. Kada a kama ta "pH Drift"

直观展示碱浸出。普通玻璃（左）会导致样品碱性漂移，而 Aijiren 硅烷化瓶（左）会导致样品碱性漂移，而 Aijiren 硅烷化瓶（烷化瓶！

Gilashin da ba a kula da shi ba yana zubar da ion alkali karfe. Ga masu nazari na hankali kamarcatecholamines ko esters, wannan motsi yana haifar da raguwa cikin sauri. Idan samfurin ku yana zaune a cikin jerin gwano, yana dafa abinci a cikin mahallin alkaline. Silanization yadda ya kamata yana rufe matrix gilashin.

5. Yaushe Ba Neman Tattaunawa?

Ba kwa buƙatar gilashin silanized don kowane gudu na yau da kullun. Amma kuna buƙatarsa sosai idan:

Abubuwan da aka tattara sune <1 μg\/mL: Asarar adsorption na mutuwa a waɗannan matakan.
Kuna amfaniLC-MS Vials: Don rage girman kololuwar fatalwa da hana ion.
AmfaniMicro-Inserts: Matsakaicin girman-zuwa-girma a nan yana da girma-idan gilashin yana aiki, samfurin ku ya tafi.
Yin ajiya tare da4ml Ajiya VialsKwanciyar kwanciyar hankali na dogon lokaci yana buƙatar jimlar tsaka tsakin sinadarai.

The Aijiren Bottom Line

Ba wai kawai mun gina vial ba; mun ƙirƙira ingantaccen yanayi don sinadarai na ku.

2 ml silanized Vials: Daidaitaccen injiniya don Agilent, Waters, da Shimadzu.
Certified Deactivation: Zero siloxane bleed into your mobile phase.
Tabbatar da Hydrophobicity: Kowane tsari an gwada kusurwar lamba don daidaitaccen ɗaukar hoto.

Dakatar da Rasa Samfuran ku - Nemi Kit ɗin Vial Silanized Kyauta Yanzu

Na baya:

Tsabtace Vial na Headspace & Sake amfani da: Gudun Aiki, Haɗarin Haɗari na Ƙirar da Za'a iya Yarwa da Sake Amfani da Vials

Na gaba:

Jagoran Kololuwa Madaidaici: Menene Reverse Phase HPLC da Yadda ake Hasashen odar Elution GC