ʻO ka hoʻomaʻamaʻa ʻana i ka Peak Precision: He aha ka Reverse Phase HPLC a pehea e wānana ai i ka GC Elution Order

I loko o ke keʻena hoʻokolohua analytical hou, ʻoi aku ka nui o ka chromatography ma mua o ka protocol maʻamau; he hulahula koʻikoʻi o ka pilina molekala. Inā ʻoe e helu ana i nā haumia lāʻau lapaʻau a i ʻole ka ʻike ʻana i nā mea haumia kaiapuni ma nā pae trace, ʻo ka hoʻomaopopo ʻana i ka physics kumu o kou kaʻawale ʻana ʻo ia ka mea e hoʻokaʻawale ai i kahi ʻenehana mai kahi haku chromatographer.

ʻAʻole loaʻa mai ka lanakila ma ka lab mai nā hoʻonohonoho mea kani kiʻekiʻe. Noho ʻo ia i ka synergy ma waena o kāu ʻano kemika a me nā mea hoʻohana kiʻekiʻe e pale ai i kāu hōʻoia pono. No ka poʻe e hoʻokele ana i ka paʻakikī o HPLC vs LC-MS: ʻO ka mea e koho ai , a i ʻole e hakakā nei me nā baseline paʻa ʻole, kaʻana kēia alakaʻi i nā mea huna o ka ʻaoʻao o ka pae i ka loaʻa ʻana o ka ʻikepili papa honua a me ka hoʻonui ʻana i ka manawa hana.

Ka hoomaopopo ana i ka Hydrophobic Handshake: He aha ka Reverse Phase Chromatography?

No ka pane ʻana i ka nīnau kumu - he aha ka reverse phase chromatography - pono e nānā kekahi ma waho o ka wehewehe puke. Ma kona kumu, he hoʻokaʻawale ia e pili ana i ka "makaʻu i ka wai" (hydrophobicity). Ma ka ʻaoʻao hoʻohuli kiʻekiʻe kiʻekiʻe kiʻekiʻe chromatography wai (RP-HPLC), hoʻohana mākou i kahi pae paʻa ʻole polar a me kahi ʻāpana polar mobile e hoʻokaʻawale i nā molekala ma ko lākou ʻano hydrophobic.

ʻO ke Kemika o ke kaulahao C18 (Octadecyl).

ʻO ka māhele paʻa maʻamau e pili ana i nā ʻāpana silica i hoʻopaʻa ʻia me nā kaulahao C18 (octadecyl). Mai koʻu ʻike, ʻo ka nui o kēia mau kaulahao C18 a me ka maikaʻi o ke kākoʻo silica e hoʻoholo ai i ke koʻikoʻi o kāu ʻano. Nui ka poʻe loiloi e hālāwai me ka huelo kiʻekiʻe i ka wā e nānā ana i nā pūhui kumu. Hoʻokumu pinepine ʻia kēia e nā pūʻulu silanol koena ma ka ʻili silica e hana ana ma ke ʻano he kahua hoʻololi ion-makemake ʻole.

No ka hoʻoponopono ʻana i kēia, hoʻohana mākou i kahi kolamu i hoʻopaʻa ʻia i ka hopena, kahi e "mask" ai nā silanes liʻiliʻi i kēia mau wahi hana. Eia nō naʻe, ʻaʻole hiki i ke kolamu maikaʻi ke hoʻoponopono i kahi ʻinikua maikaʻi ʻole. Inā ʻano ʻino kāu piko chromatographic ma mua o ka pā ʻana i ke kolamu, e nānā i kāu hue. Ke hoʻohana nei i ka maʻemaʻe kiʻekiʻe 9mm Pokole Uila Uila me Universal Fit e hōʻoia ʻaʻole e hoʻopili ʻia kāu analyte i nā paia vial ma mua o ka lawe ʻana i ka nila.

ʻO ke Kiʻi o ka Mea Hoʻololi Organic ma HPLC Method Development

Hoʻomalu ʻia ka manawa paʻa o kāu mau analytes e ka hoʻololi hoʻololi kūlohelohe HPLC. Ma ka hoʻololi ʻana i ka ratio o Acetonitrile a i ʻole Methanol i kāu paʻa wai, hoʻokani ʻoe i ka "ikaika elution" o ka pae paʻa.

ʻO kahi pitfall maʻamau aʻu i ʻike ai ma ka hoʻololi ʻana i ke ʻano, ʻo ia ka hoʻomaopopo ʻole ʻia o ka gradient elution curve. Inā wikiwiki ʻoe e hoʻololi i kāu mea hoʻololi organik, nalowale ʻoe i ka hoʻonā; lohi loa, a ua pilikia ʻoe i ka hoʻonui ʻana i ka piko a me ka hoʻoheheʻe ʻia. Ke hoʻololi i waena ʻIkepili vs Hoʻomākaukau HPLC , 'o ka ho'omalu 'ana i ka gradient slope ke kumu nui o ka ho'onui 'ana i ka holomua. E hoʻomanaʻo, hāʻawi ʻo Acetonitrile i ka viscosity haʻahaʻa a me ka ikaika elution kiʻekiʻe, ʻoiai hiki i ka Methanol ke hāʻawi i nā koho koho ʻokoʻa no nā pūhui polar i hala ʻole ai ʻo ACN.

Ka Hoʻokaʻawale ʻana i ka GC Elution Order: Predicting Compound Separation

ʻO ka wānana ʻana i ke kauoha elution gc kekahi o nā luʻi ʻoi loa no ka chromatographer kinoea. ʻAʻole like me HPLC, kahi i hoʻoheheʻe ʻia ka mea hoʻoheheʻe kemika ka lever mua, ʻo ka hoʻokaʻawale ʻana o GC e kuhikuhi ʻia e ka "Trinity of Separation": kahi paila, polarity molecular, a me ka papahana wela kolamu.

ʻO Thermodynamics vs. Dipole Interactions

Ma kahi ʻāpana kū ʻole polar, ʻo ke kauoha gc elution e hahai pono i ka helu paila. ʻO nā mea hoʻohui like ʻole e puka mua. Eia naʻe, loli ka pāʻani ke hoʻohana ʻoe i kahi kolamu polar e like me PEG / Wax. Ua lawelawe au i kēia manawa i kahi papahana e pili ana i nā isomers kahi i ʻaneʻane like nā wahi paila. Ma ka hoʻololi ʻana i kahi ʻāpana polar, ua ʻae nā pilina dipole-dipole iā mākou e hoʻokaʻawale i kēia mau pūhui e pili ana i kā lākou hana uila ma mua o ko lākou nui.

No ka hana kiʻekiʻe e like me ka nānā ʻana i ka trace kaiapuni, ʻo ka pono o kāu ipu laʻana ka mea nui. Ke hoʻohana nei 20mm Crimp Top Headspace Vials pale i ka nalowale ana o nā analytes volatile, e hōʻoia ana i kou nuipa spectral deconvolution ua hoʻokumu 'ia ma luna o ka 'oiaʻiʻo hōʻike o kou hāpana, 'aʻole he leaked hakina.

HPLC vs LC-MS: Ke hoʻokele nei i ka Hoʻololi Manaʻo

ʻO kahi nīnau pinepine i kā mākou lab: "I ka manawa hea mākou e neʻe ai mai HPLC vs LC-MS?" Aia ka pane i kāu ʻike pono. ʻOiai maikaʻi loa ka HPLC me ka ʻike UV no ka QC maʻamau ma nā pae microgram, pono ʻo LC-MS inā pono ʻoe e ʻike i nā pae picogram a i ʻole e ʻike i nā mea ʻike ʻole i nā matrices paʻakikī.

Inā e neʻe ana ʻoe i LC-MS, ʻoi aku ka koʻikoʻi o kāu koho ʻana i nā hue. Hiki i nā pāpale maʻamau ke hoʻoheheʻe i nā plasticizers i kāu hana paʻa, e hana ana i "Ghost Peaks" e hauʻoli i kāu chromatogram. ʻO kēia ke kumu o Aijiren Paʻa Paʻa Paʻa He mea koʻikoʻi ia i nā labs i hōʻoia ʻia e MS-e hoʻopau lākou i ka pilikia o ka hāʻule ʻana o ka septa i loko o ka vial a i ʻole ka hoʻolei ʻana i nā mea haumia i loko o ka mea ʻike.

ʻO ke Anatomy o kahi kiʻekiʻe Chromatographic Pono

ʻO kēlā me kēia peak chromatographic kahi mea hana diagnostic. Hōʻike ʻia kahi kiʻekiʻe Gaussian ʻoiʻoi a symmetrical ua hoʻomaikaʻi ʻia kāu ʻōnaehana. ʻO ka "poʻohiwi" a i ʻole ka "huelo" e haʻi iā ʻoe ua hewa kekahi mea, pili pinepine i ka hāmeʻa a i ʻole ka leo make.

E hoopau ana i ka Volume Make a me ka Volume Extra-Column

ʻO kekahi o nā mea pepehi leo o ka hoʻonā ʻana he leo make. Inā loaʻa kekahi ʻokoʻa ma waena o kāu nila a me ka lalo o ka hue, hoʻopuehu ka hāpana. No ka micro-sampling, paipai mau au i ka hoʻohana ʻana i Conical Inserts. Hoʻokomo kēia mau mea hoʻokomo i ka hāpana i kahi ala haiki a kū pololei, e hōʻoia ana i kahi hoʻokomo "koi". Hoʻopuka pololei kēia i kahi piko ʻoi aku ka maikaʻi a ʻoi aku ka maikaʻi o ka hōʻailona-a-noise ratio, ʻoi aku hoʻi i nā ʻōnaehana kiʻekiʻe kahi e hoʻopuehu ai ka ʻenemi.

ʻO ka hoʻoponopono ʻana e like me ka Pro: Mai ka Baseline Noise a Septa Coring

I kaʻu mau makahiki o ka hoʻoponopono ʻana, ua ʻike wau he 70% o nā pilikia mea hana he mau pilikia hoʻopau.

1. Baseline Noise & Ghost Peaks: Inā ʻike ʻoe i nā piko ma kahi ʻaʻohe mea, ʻo kāu kānana paha ka mea hewa. E nānā mau ʻO ke alakaʻi piha i nā kānana 0.22 Micron ma mua o ke koho ʻana i kāu mau kānana syringe.

2. Septa Coring: Inā ʻike ʻoe i nā ʻāpana o ke silikoni i loko o kāu hue, ʻo kou nila ke "hoʻokiʻoki" i ka septa. Hana ʻia kēia me nā pāpale maikaʻi ʻole. ʻO kā mākou ND11 Crimp Caps a me 10-425 Screw Caps i hana pololei ʻia e pale i nā punctures me ka ʻole o ka ʻāpana.

3. Paʻa i ka mālama ʻana: No nā ʻano EPA a i ʻole ka mālama ʻana i ka wā lōʻihi, me ka hoʻohana ʻana 24-400 EPA Vials i hana ʻia me ke aniani borosilicate e hōʻoiaʻiʻo i ka nalowale o ka solvent a me ka contamination ʻole.
   
   

No ke aha e hoʻomaka ai ka pono o ka ʻikepili me ka mea hoʻohana

Hoʻohana pinepine mākou i ka 50,000 USD ma kahi ʻōnaehana kiʻekiʻe, a laila e hoʻāʻo e mālama i nā peni ma ka vial. I koʻu ʻike, ʻo ka sub-par vial ka mea kūʻai nui loa i kāu lab no ka mea e alakaʻi i ka "holo hou" a me nā "hōʻoia ʻole."

Inā makemake ʻoe i 1ml Shell Vials no ka chromatography maʻalahi a i ʻole 18mm Screw Thread Headspace Vials no GC automated, hāʻawi ʻo Aijiren i ka kūlike a nā poʻe loea e koi ai. Hoʻopaʻa mākou e hana hou ʻia kāu kauoha gc elution i kēlā me kēia lā, a ʻoi aku ka ʻoi o kou piko chromatographic e like me ka lā āu i kūkulu ai i ke ʻano.

Kākoʻo a me nā nīnau nīnau: Ke alo nei ʻoe i nā hopena like ʻole a i ʻole e hakakā nei me ka hoʻomohala ʻana i ke ʻano? E hoʻoikaika pū kāua i kāu kaʻina hana. E kelepona mai iaʻu no ke kūkākūkā ʻenehana:

• WhatsApp:+86 18338832256

• leka uila:boonemi@aijirenvial.com

Manaʻo Manaʻo ʻO ka hoʻomaʻamaʻa ʻana i ka pae hope a me ka hoʻomaʻamaʻa ʻana i nā nuances o kāu mea kani he huakaʻi o ke aʻo mau ʻana. Ma ke koho ʻana i ka HPLC hoʻololi kūlohelohe kūpono a me nā mea hoʻohana maikaʻi loa i ka papa, ke kālele nei ʻoe i ka ʻoiaʻiʻo o kāu ʻikepili. Mai ʻae i kahi hue sub-par e hōʻino i kahi ala maikaʻi āu i hoʻopau ai i nā hebedoma e ulu ana.