High Performance Liquid Chromatography (HPLC) is an important analytical technique used in various fields such as pharmaceuticals, environmental analysis, and food safety. Ib qho tseem ceeb ntawm kev hplc system yog lub xeem septum, uas ua haujlwm ua kev kaw rau lub vial. Lub xeem septum yog qhov kaw tias cov ntsaws ruaj ruaj qhib, tso cai koob muab tso rau hauv kev tswj hwm kev nyab xeeb thiab evaporation. Lawv yuav tsum sib haum nrog cov kuab tshuaj ntsuam xyuas thiab yuav tsum tau tiv thaiv cov txheej txheem HPLC cov txheej txheem.

Qhov tseem ceeb ntawm kev xaiv txoj cai septa

Xaiv txoj cai septum yog qhov tseem ceeb rau:

Kev tiv thaiv kev ua kom tiv thaiv kev paug: cov tshuaj tsis sib xws tuaj yeem pom rau hauv cov qauv lossis kev hnov mob nrog cov kuab tshuaj.

Ua kom muaj lub cim kom tsim nyog: ib lub foob zoo tiv thaiv cov qauv poob thiab tswj hwm kev ncaj ncees.

Txhawb nqa ntau txoj kev txhaj tshuaj: qee qhov septa yog tsim los ua kom rov siv tau yog tsis muaj kev nyuaj siab.

Xav paub tag nrho cov kev paub txog PTFE \ / Silicone sterta, thov xyuas cov ntawv no: Txhua Yam Koj Yuav Tsum Tau Paub: 137 Pre-SLIT PTFE \ / Silicone Septa Soutra Faqs

HPLC Vial sainta khoom siv

Cov ntaub ntawv sib txawv muaj cov khoom sib txawv uas cuam tshuam rau lawv cov kev ua tau zoo hauv HPLC daim ntawv thov. Cov hauv qab no yog cov ntaub ntawv feem ntau siv hauv HPLC Vial sterta:

1. PTFE (polytetrafluoroethylene)

Nta: PTFE muaj cov tshuaj kuj zoo heev, yog qhov ua tsis tau zoo thiab tuaj yeem tiv taus cov kuab tshuaj nruj.

Siv: Tsis tsim nyog rau ntau qhov kev txhaj tshuaj lossis lub sijhawm khaws cia ntev rau nws qhov tsis muaj peev xwm rov qab ua tiav tom qab raug kaw, ua rau nws zoo rau ib leeg siv.

Kev kub siab kuj: withstands kub txog 260 ° C.

Tus nqi: Feem ntau kev lag luam piv rau lwm cov ntaub ntawv.

2. PTFE \ / Silicone Septa

Nta: sib txuas cov tshuaj lom neeg tiv thaiv PTFE nrog lub rov qab los ntawm silicone.

Siv: Pom zoo rau ntau qhov kev txhaj tshuaj thiab cov qauv cia vim nws qhov kev rov qab zoo dua.

Kub Ntau: Tau txais txiaj ntsig los ntawm -40 ° C txog 200 ° C.

Autoclavable: Haum rau cov txheej txheem kom tsis muaj menyuam.

3. Ua Ntej Txiav PTFE \ / Silicone Septa

Nta: Tsim los txo cov coring thaum siv blunt-tipped koob; Txo lub tshuab nqus tsev tsim nyob rau hauv vials.

Siv: Tsim nyog rau cov ntawv thov qhov twg nquag tiv toj yuav tsum tsis muaj kev sib txawv ntawm septum ncaj ncees.

Ntau yam: zoo ib yam li PTFE \ / Silicone, -40 ° C txog 200 ° C.

4. Butyl Roj Hmab

Cov Khoom: Kev txiav sealing zoo tshaj plaws, tab sis kev tshuaj tiv thaiv tsawg dua piv rau PTFE.

Siv: Tsim nyog rau cov kev daws teeb meem aqueous thiab tsawg dua cov kuab tshuaj; tuaj yeem siv rau hauv cov ntawv thov uas xav tau lub foob pob cua.

Kev kub siab kuj: zoo ntawm qis dua kub, tab sis tuaj yeem ua rau muaj kev cuam tshuam ntawm cov cua sov lossis nrog cov kuab tshuaj hnyav.

5. Koob hmoov

Cov Khoom: Muaj zog tiv thaiv kev txhoj puab heev thiab khaws qee lub peev xwm los rov ua haujlwm tom qab puncture.

Siv: suav tias yog lub sijhawm kawg vim yog tus nqi siab; Haum rau cov ntawv thov uas lwm cov ntaub ntawv tau ua tsis tiav.

Cov kub ua hauj kub ua kom tau: Zoo nyob rau ntawm qhov kub siab, tab sis yuav tsum tau ua tib zoo tuav vim nws lub rigidity.

Yam los txiav txim siab thaum xaiv HPLC Vial sentera

1. Tshuaj Zoo

Thawj qhov uas yuav tsum xav txog yog cov tshuaj sib xyaw ua ke ntawm cov khoom siv septum nrog cov kuab tshuaj koj npaj siv. Cov ntaub ntawv sib txawv muaj cov tshuaj tiv thaiv txawv rau cov tshuaj:

PTFE (Polytetrafluoroethylene): PTFE paub txog nws cov tshuaj lom neeg ua tau zoo thiab yog qhov haum rau ntau hom kuab tshuaj, suav nrog cov tshuaj corrosive. Nws feem ntau yog siv hauv kev sib xyaw nrog lwm cov ntaub ntawv.

Silicone: Silicone Sepico yog hloov tau thiab tuaj yeem rov qab tau tom qab rab koob nkag mus, ua rau lawv haum rau kev txo cov tshuaj. Txawm li cas los xij, lawv yuav tsis sib haum nrog txhua cov kuab tshuaj.

Butyl Roj Hmab: Cov khoom no muaj kev sib khi zoo, tab sis muaj cov tshuaj tiv thaiv kev tiv thaiv rau PTFE.

PTFE \ / Silicone Composite: ua ke cov txiaj ntsig ntawm ob cov ntaub ntawv, nrog tshuaj lom neeg zoo thiab rov ua dua.

2. Cov Hom Hom

Hom tshuaj siv yuav cuam tshuam rau kev xaiv Septum:

Cov tshuaj aqueous: rau cov kuab tshuaj-silicone, silicone lossis ptfe \ / Silicone sitta feem ntau pom zoo vim lawv zoo heev.

Organic cov kuab tshuaj: rau cov kuab tshuaj organic xws li Methanol lossis Acetoniturile, PTFE lossis PTFE lossis PTFE COMPOSites yog nyiam vim lawv cov tshuaj tiv thaiv zoo heev.

Corrosive tshuaj: rau cov kuab tshuaj muaj zog xws li muaj zog acids lossis hauv paus, PTFE feem ntau yog qhov kev xaiv zoo tshaj plaws.

3. Kev Ua Lag Luam

Lub peev xwm ntawm septum los pib tom qab rab koob puncture yog qhov tseem ceeb kom ua qauv ncaj qha:

Slit vs. Cov qauv kev tsis sib tw: qee qhov septa muaj kev sib tsoo kom pab txhawb koob ntxig, tab sis qhov no yuav cuam tshuam rau lub foob nyob rau lub sijhawm. Cov qauv tsis-hlais muab cov foob tig rov qab tab sis tej zaum yuav tsum tau quab yuam ntau dua thaum txhaj tshuaj.

Rov qab pib: Saib rau septa uas tuaj yeem rov qab zoo dua tom qab ntau rab koob kom tiv thaiv kev sib kis thiab evaporation.

Xav paub seb puas yuav xaiv septa nyob rau hauv pre-slit lossis tsis? Thov kos kab lus no: Yuav ua li cas xaiv Septa Pre-Slit lossis tsis?

4. Extractables thiab ntshagables

Thaum ua haujlwm nrog cov qauv rhiab, nws yog qhov tseem ceeb rau kev txiav txim siab cov extractables thiab leachables hauv cov khoom siv septum:

Tsawg Extractables: Xaiv Septa nrog cov khoom siv qis qis los txo qhov kev pheej hmoo ntawm kev paug. PTFE muaj cov khoom siv tsawg dua piv rau cov khoom siv roj hmab-burter.

Cov qauv xeem: Qee cov tuam ntxhab muab cov ntaub ntawv ntawm cov extractables; Txheeb xyuas cov specifications no thaum xaiv septum.

5. Kev kub siab kuj tsis kam

Xav txog qhov ntsuas kub uas koj lub zog HPLC yuav ua haujlwm:

Kev kub siab: Yog tias koj daim ntawv thov cuam tshuam nrog kev kub siab, nco ntsoov cov khoom siv septum tuaj yeem tiv thaiv cov mob no yam tsis muaj kev ua tsis txaus ntshai.

Cryogenic daim ntawv thov: rau cov ntawv thov cryogenic, xaiv cov khoom uas tswj hwm kev ncaj ncees ntawm qhov kub tsawg.

6. Lub cev muaj zog

Cov yam ntxwv ntawm lub cev kuj tseem yuav cuam tshuam kev ua tau zoo:

Thickness: Thicker septa yuav muab lub foob zoo dua tab sis yuav xav tau ntau zog rau lub zog kev nkag mus.

Cov khoom siv tawv tawv: Cov ntaub ntawv softer yuav muab cov khoom rov qab zoo dua tab sis yuav raug rau kev raug mob ntau dua thaum siv tas.

Xaiv txoj cai HPLC Vial Septum yuav tsum tau saib xyuas cov kev xav tau zoo, kev sib sau ua ke, muaj cov kev xav tau kub thiab cov kev xav tau. Los ntawm kev nkag siab cov khoom siv ntawm cov ntaub ntawv sib txawv thiab lawv cov ntawv thov, cov neeg tshawb xyuas tuaj yeem txhim kho qhov tseeb thiab kev ntseeg tau ntawm HPLC analyzes. Txoj cai xaiv tsis tsuas txhim kho kev ua tau zoo, tab sis kuj txo cov kev pheej hmoo cuam tshuam nrog piv txwv tsis ua haujlwm thiab kev paug.

Yav dhau los:

Vim li cas cov khoom muaj txiaj ntsig high-High

Tom ntej:

Flash chromatography vs. HPLC: Cov ncauj lus piav qhia ntxaws