Koj puas paub txog daim ntawv chromatography?

Daim ntawv chromatography yog cov txheej txheem ntsuas dav hlau uas cais thiab paub txog cov tshuaj sib xyaw ua ke raws li cov theem sib txawv rau cov theem nyob ruaj khov (ntawv) thiab mob hnyav). Nws yog pab tshwj xeeb hauv Chemistry thiab biochemistry rau kev tshuaj xyuas cov tshuaj me xws li pigments, amino acids, thiab lwm cov organic sib sau.

Dab tsi yog daim ntawv chromaphy?

Daim ntawv chromatography yog ib hom kua chromatography nyob rau hauv uas yog daim ntawv ntawm cov ntawv lim thiab mobile theem yog cov lus qhia uas ntws los ntawm daim ntawv lim. Cov txheej txheem ntawm cov txheej txheem sib txawv ntawm cov sib txuas ntawm cov theem nyob nruab nrab (lim txheej txheem) thiab cov kuab). Raws li cov kuab tshuaj txav mus txog daim ntawv lim cov ntawv los ntawm capillary txiav txim, nws nqa cov khoom sib xyaw ntawm cov sib xyaw ntawm cov sib txawv ntawm ob ntu.

Cov ntsiab cai hauv qab daim ntawv chromatography tuaj yeem to taub los ntawm ob lub ntsiab lus tseem ceeb: muab faib cov chromatography thiab adsorption chratography.

Muab faib cov chromatography: hauv hom no, cov tshuaj tau faib ntawm ob theem ua kua. Theem nyob ruaj khov muaj cov dej hauv cov pores ntawm daim ntawv lim, thaum lub sij hawm mobile theem yog cov hnyav uas txav los ntawm cov hws no. Kev sib cais tshwm sim vim hais tias cov sib txawv muaj sib txawv nrog kev sib txawv rau lub tshooj lus nyob ruaj ruaj thiab ntsuas hluav taws xob.

Adsorption chrataphy: Ntawm no, cov khoom nto ntawm cov ntawv ua raws li theem nyob ruaj khov thiab cov kua kuab dej ua raws li lub xov xwm mobile. Cov Cheebtsam hauv cov dej sib tov tau sib cais raws lawv cov adsorption rau cov khoom saum npoo av thiab lawv cov solubility hauv cov theem ua kua.

Cheebtsam

Theem nyob ruaj ruaj: cov ntawv tshaj tawm, feem ntau yog ua los ntawm cellulose. Nws muab saum npoo rau cov khoom siv rau ua raws li.

Mobile theem: cov kuab tshuaj lossis sib xyaw ntawm cov kuab tshuaj uas txav ntawm daim ntawv los ntawm kev ua yeeb yam. Qhov kev xaiv ntawm cov kuab tshuaj tuaj yeem cuam tshuam qhov cuam tshuam ntau ntxiv cuam tshuam rau kev sib cais.

Qauv: Qhov sib xyaw kom sib xyaw kom raug tshuaj xyuas, feem ntau pom ntawm daim ntawv pib pib ntawm daim ntawv.

Xav paub ntau ntxiv txog HPLC qauv npaj, thov tshawb xyuas kab lus no: HPLC tus qauv npaj cov kev daws teeb meem rau cov txiaj ntsig zoo tshaj plaws

Daim ntawv chromatography cov txheej txheem

Kev npaj: xaiv ib daim ntawv lim dej zoo nrog lub porosity kom ntseeg tau tias kev sib cais zoo. Siv ib feem me me ntawm cov qauv sib xyaw ua ke rau cov hauv paus daim ntawv ntawm daim chromatography.

Qauv npaj: yaj cov qauv sib tov nyob rau hauv qhov tsim nyog hnyav ua ib feem ntawm lub mobile theem.

Spotting: Siv cov capillary lossis micropipette kom poob ib qho kev daws teeb meem me me ntawm cov hauv paus ntawm daim ntawv lim.

Kev loj hlob: Poob hauv qab ntug ntawm cov ntawv xeem rau hauv kev tsim cov kuab tshuaj (mobile theem). Raws li kev ua yeeb yam hauv capillary tshwm sim, cov kuab sawv los ntawm cov ntawv xeem, nqa cov qauv sib xyaw nrog nws.

Kev sib cais: raws li cov kuab tshuaj txav, sib txawv ntawm cov qauv yuav txav ntawm tus nqi sib txawv vim yog theem chaw nyob thiab sib txawv hauv cov theem mobile.

Kev pom tau: tom qab cov kuab tshuaj pem hauv ntej tau txav mus deb, daim ntawv raug tshem tawm ntawm cov kuab tshuaj thiab sab laug kom qhuav. Cov khoom siv tuaj yeem pom tau siv UV teeb, tshuaj lom neeg tshuaj lom neeg, lossis los ntawm kev tsom iav hloov.

Kev tsom xam: qhov kev ncua deb los ntawm txhua tus neeg txheeb ze rau cov kuab tshuaj yog ntsuas los laij cov nqi RF (RESENTENTION.

Cov ntawv thov ntawm daim ntawv chromatography

Daim ntawv chromatography muaj ntau cov kev siv hauv ntau thaj teb:

Biochemistry: Kev soj ntsuam ntawm cov amino acids, suab thaj, thiab lwm cov biomolecules.

Kws Tshuaj: Cim ntawm cov khoom xyaw nquag thiab cov kev tsis sib haum xeeb hauv cov qauv tshuaj. Siv los txheeb xyuas cov chaw ua haujlwm thiab kev tsis sib haum xeeb hauv cov qauv tshuaj thaum lub sijhawm kev txhim kho thiab kev tswj hwm kom zoo.

Kev tshawb fawb ib puag ncig: cov ntawv chromatography yog siv los tshawb xyuas cov khoom paug hauv cov dej paug thiab tshuaj pleev cov paug hauv av rho tawm.

Kev Lag Luam Khoom Noj: Cov txheej txheem kev pab hauv kev tshuaj ntxiv ntxiv, cov paug, thiab cov xim hauv cov khoom noj kom ua raws li kev nyab xeeb.

Qhov zoo ntawm daim ntawv chromatography

Tus nqi ua tau zoo: nws yuav tsum tau siv cov khoom siv tsawg heev thiab cov ntaub ntawv piv nrog rau lwm cov tswv yim chromatographic xws li HPLIraphic cov tswv yim zoo li cov chromatography). Simplicity: Cov txheej txheem yooj yim heev thiab tuaj yeem ua nrog cov cuab yeej ntawm cov chaw kuaj, yog li nws tuaj yeem siv rau kev kawm.

Cov qauv qis uas yuav tsum tau ua: tsuas yog cov qauv me me yog qhov yuav tsum tau ua rau kev tsom xam, ua rau nws zoo rau cov qauv muaj nqis lossis txwv.

Versatility: Nws tuaj yeem sib cais ntau cov tshuaj ntawm cov tshuaj raws ntawm cov tshuaj, suav nrog ncej polar, suav nrog cov ncej puab thiab tsis yog-polar

Kev txwv ntawm daim ntawv chromatography

Txawm hais tias nws zoo, ntawv chromatography muaj qee qhov kev txwv:

Muaj peev xwm tsawg tsawg: feem ntau haum rau cov qauv me me.

Kev daws teeb meem: Qhov kev daws teeb meem yuav tsis muaj siab npaum li qhov ua tiav los ntawm ntau cov txujci siab xws li HPLC lossis roj chromatography (GC).

Sij hawm siv tau: Vim tias qeeb qeeb tsiv teb tsaws, cov txheej txheem yuav siv sijhawm ntau dua rau lwm cov chromatographic.

Tsawg txoj kev tsom xam feem ntau: thaum cov txiaj ntsig kev tsim nyog tuaj yeem tau yooj yim, cov qauv ntsuas hluav taws xob ntxiv tuaj yeem ua rau muaj kev ntau ntau.

Daim ntawv chromatography tseem muaj cov txheej txheem tseem ceeb rau kev sib cais thiab kev txheeb xyuas cov kev sib xyaw ua ke hauv thaj chaw science. Nws yooj yim, nqi-ua hauj lwm zoo, thiab muaj peev xwm muab cov txiaj ntsig zoo ua kom nws cov cuab yeej tseem ceeb rau cov kws kho mob thiab cov kws tshawb nrhiav. Txawm hais tias nws cov kev txwv piv rau ntau dua cov kev siv cov tshiab

Xav paub 50 cov lus teb txog HPLC Vials, thov tshawb xyuas kab lus no: 50 Feem Ntau Cov Lus Nquag Nug ntawm HPLC Vials