Dab tsi yog qhov txawv ntawm GC-MS thiab GC-MS \ / ms?

Roj chromatography-mass spectrometry (GC-MS) thiab roj Chromatographes siv nyob rau hauv cov chaw ua haujlwm, ib puag ncig kev tshawb fawb, kev nyab xeeb ib puag ncig, kev nyab xeeb ntawm kev nyab xeeb, thiab muaj kev nyab xeeb. Ob cov txuj ci sib xyaw ua ke cov chromatographic sib cais nrog cov roj hluav taws xob ntau tab sis qhov txawv hauv kev ua haujlwm, rhiab heev, muaj feem tshwj xeeb, thiab kev siv.

1. Tus GC-MS yog dab tsi?

GC-Ms cov khub niam txiv chromatography, uas cais cov ntsiab lus tsis sib xws los ntawm cov chaw kub hnyiab, uas yog tus cim tau ntau yam kev sib txuas ua ke raws li huab hwm coj los them (m \ / z) sib piv

Cov khoom tseem ceeb:

• Roj Chromatograph: Cais sib txuas ntawm cov sijhawm khaws cia.

• Huab hwm coj hluav taws xob: ionizes cov tebchaw (feem ntau ntawm cov tshuab hluav taws xob ionization, EI) thiab tsim tawm Spectra
  

Kev Npaj Qauv:

Xws li kev lim tawm, khoom theem rho tawm (spe) lossis kua-kua extraction (lle), thiab derivatization kom teb gc-npaj.

2. Dab tsi yog GC-ms \ / ms?

GC-MS \ / MS qhia theem ob ntawm kev tshuaj ntsuam xyuas loj. Tom qab kev xaiv ion thawj zaug hauv thawj quadrupole (Q1), xaiv Eions Force kev sib tsoo - cid) hauv kev sib tsoo ntawm tes. Qhov nruab nrab ions yog ces tshuaj ntsuam los ntawm lub quadromole thib ob (q2). Qee lub tshuab yuav suav nrog tus thib peb quadrupole lossis tag nrho analyzer

Txhim Kho Kev Ua Tau:

• Qhov tshwj xeeb tshaj plaws thiab kev rhiab heev, tshwj xeeb los ntawm ntau ntawm ntau cov tshuaj tiv thaiv kev saib xyuas (MRM)

• Ua tau zoo nyob rau hauv cov nyom matrices, muab cov cim qhia meej uas ib qho GC-MS yuav tawm tsam
  
  

3. Kev thov

GC-MS:

Feem ntau siv rau:

• Cov Kev Ntsuam Xyuas Cov Lus Nug

• Forensic kev tshawb nrhiav

• Khoom Noj Khoom Noj Kom Zoo thiab qhabnias

• Kev ntsuam xyuas ib puag ncig ib puag ncig

GC-MS \ / MS:

Qhov zoo tagnrho rau:

• Qeb Quantification (piv txwv li, tshuaj tua kab ntawm PPT theem)

• Complex matrices qhov twg co-elution tshwm sim.

• High-fructed tshuaj tua cov tshuaj tua neeg siv mrm.

• Cov ntawv thov siab heev, xws li cov khoom noj ua kom muaj cov khoom noj thiab ib puag ncig txoj kab xev.
  
  

  

4. Qhov sib txawv tseem ceeb

5. Cov Lus Nug

Q1: Dab tsi yog qhov tseem ceeb tshwm sim ntawm GC-MS thiab GC-MS \ / ms?
A1: GC-MS \ / MS muaj kev txhim kho tshwj xeeb thiab rhiab los ntawm huab hwm coj ntxiv thiab cov theem zoo tagnrho rau cov qauv nrhiav kom pom

Q2: Thaum twg GC-MS nyiam?
A2: Kev tsom xam cov organic uas muaj kev xav tau thaum muaj kev xav tau yog qhov me me thiab matrices yog yooj yim.

Q3: Thaum twg kuv yuav tsum tau siv GC-MS \ / ms?
A3: Rau Ultra-trace kom paub tseeb hauv cov qauv sib piv, ib puag ncig, qib siab-kev tawm mus kuaj, thiab kev txheeb xyuas qhov tseeb.

Q4: Cov GC-MS Siv cov khoom sib txuas tsis muaj zog?
A4: tsis ncaj qha; derivatization yuav tsum. Rau qhov loj dua lossis thermally labile tebchaw, LC-MS lossis LC-MS \ / MS yuav tsim nyog dua.

Q5: Cov nqi sib piv li cas?
A5: GC-MS cov cuab yeej siv feem ntau tsawg dua kim (kaum tawm txhiab tus USD). GC-MS \ / MS systems (nrog QQQ lossis ion Tric kev teeb tsa) raug nqi ntau dua tab sis muaj kev ua haujlwm zoo dua rau kev ntsuas tshwj xeeb

GC-MS thiab GC-MS \ / ms ob qho tib si muaj kev sib zog ua kom muaj zog. GC-MS \ / MS Ntxiv kom zoo nkauj, xaiv tau, thiab kev pom muaj kev paub zoo-tshwj xeeb muaj txiaj ntsig hauv qib siab, kev ntsuas siab. Kev xaiv yuav tsum yog ua raws li cov khoom ntiag tug xws li cov hom phiaj anyalytes, cov qauv sib zog, kev txwv tsis pub dhau, dhau los yuav tsum muaj, thiab pob nyiaj siv.

Saib cov lus qhia tiavDab tsi yog qhov txawv ntawm GC-MS thiab GC-MS \ / ms?

1. Chromatographline. Flying high nrog kev xaiv thiab xaiv: GC-MS rau GC-MS \ / MS (chromatographyOnline.com)

2. Wiley Analytical Science. Cov kev sib piv ntawm kev sib piv ntawm GC-MS nrog EI (analyticalscenceJournals.onlinelibrary.wiley.com)

3. Agilent gc-ms faqs (Agilent.com)

4. Wily. Tandem Pawg Loj Spectrometry (en.wikipedia.org)

5. NCBI PMC. GC-MS \ / MS TSHUAJ TSHUAJ POV TSHUAJ NTAWM ATMOSPERCE PARDENTS (pmc.ncbi.nih.gov-gov)