Kev Npaj Qauv Qauv & Pom Kev: Ua kom zoo hauv cov chromatography

Cov qauv tsim nyog ua ntej cov thawv ntim khoom, tshem tawm cov partalulates, thiab, yog tias xav tau, ua rau muaj lub zog, ua kom muaj zog, ua kom muaj txiaj ntsig ntse thiab cov hauv paus ruaj khov. Ua tsis tiav ntawm cov txiaj ntsig hauv kev ua kom Tailing, Drifting hauv paus, lossis kab ntawv thaiv.

1. Kev paug tsis muaj kev cuam tshuam & matrix cuam tshuam

Teeb meem

• Baseline drift thiab rising lub suab nrov tsis pom kev me me.

• Kev npaj txhij txog peaks pom los ntawm leached impurities.

• Pluag reproducibility ntawm kev txhaj tshuaj.
  

  

Kev kom

• Cov chaw nyob hauv cov iav: tsis tiav cov kua qaub-ntxhua khaub ncaws cov thawv silicates; Cov khoom siv kom yooj yim tuaj yeem ua kom cov khoom ua kom huv.

• Kev tu cov neeg sawv cev: Khwv cov orgysics hauv Rinse cov kuab tshuaj nyob twj ywm adsorbed.

• Ib puag ncig cov particulates: lab air hmoov av thiab vocs adsorb mus rau qhov chaw qhib.
  

  

Lus teb

1. Nruj tu sop: Acid soak → Ultrasonic ntshiab-dej yaug → nitrogen qhuav rau cov iav; Dedicated xab npum rau plastics.

2. Kev ua ntej pom: Centrifuge ntawm 3000 teev rau 10min lossis siv 500M Prefilter ua ntej 0.22μm pom.

3. Tswj ib puag ncig: Ua cov khoom ua ntej hauv Laminar-ntws hood kom txo qis kev paug huab cua kom tsis huv.
   
   

2. Lim daig los ntawm cov ntxaij lim dej tsis sib xws

Teeb meem

• Autosampler siab tswb thiab nquag hloov kev hloov pauv.

• Txo tus nqi txaus, tsis sib xws thaj chaw.

• Txhawb cov rompture thiab kem inlet blockage.
  

  

Kev kom

• Membrane Mismatch: Nylon swells hauv cov kuab tshuaj organic; PVDF nkag rau ntawm qhov kub thiab txias.

• Tsis muaj tseeb lub siab loj: 0.22μm Lim Nkaum cov particulates tab sis txo qis; 0.45μm lim tej zaum tseem yuav txhaws nrog microcrystals.

Lus teb

1. Membrane-solvent matrix: Siv Hydrophilic Pes \ / MCE rau AQUOATE (PH4-8), Hydrophobic PTFE \ / PVDF rau theem organic; Zam kev cellulose acetate hauv cov acids muaj zog.

2. Xaiv Zoo Pore Loj: Siv 0.45μm rau kev niaj hnub HPLC, 0.22μm rau UHPLC lossis kab haujlwm ua haujlwm; Advilter High-voscority cov qauv mus txog 5μm.

3. Saib xyuas Δp: Taug qab lub siab sawv; Hloov cov lim thaum raispressiture nce ntxiv> 15%.
   
   

   

3. Kuab-Membrane Tshuaj Tsis Sib Tau

Teeb meem

• Cov qauv lim tshwm li huab lossis nqa cov lus.

• Cov suab nrov tshiab lossis dab Peaks Peaks-Pom Kev Pom Zoo.

• Txo kev ntsuas ntsuas ntsuas.
  

  

Kev kom

• Cov kuab ntshav o: Thf swells PTFE, hloov PEt loj thiab txaus.

• Membrane Leachables: Nylon hauv formic acid tso tawm oligomers uas nqus ntawm 214nm.

Lus teb

1. Kev Xeem Sib Xws: Soak lim hauv cov kuab tshuaj rau 24h ntawm 25 ° C; Soj rau o lossis hnyav hloov.

2. Pilot LC-MS TEST: Sib piv tag nrho ion ChromatoGrams ntawm lim thiab tsis ua qauv.

3. Siv cov lim lim dej tsawg: Ultrasonic-welded PTFE lossis pes tsis muaj cov nplaum.
   
   

Txheej Txheem Zoo: Kev Tiv Thaiv Kev Tiv Thaiv-Kho

Qhov tiv thaiv

Qauv cov sops kom ntxuav, pom, xaiv membrane xaiv tiv thaiv tsis raug.

Xyuas

Cov ntaub ntawv txaus txaus, rapperture, thiab dawb paug-piv txwv ntawm cov hauv paus los kuaj cov drift.

Kev kho

Tswj lub txim ntoo kev txheeb xyuas rau cov hauv paus hniav sai sai thiab kho kom raug.

"Kev tshawb xyuas precision pib nrog cuam tshuam cov me me particle."