Xaiv txoj cai tsis txaus rau koj HPLC Mobile theem yog qhov tseem ceeb kom ua tiav kev sib cais zoo tshaj plaws thiab kev tshuaj ntsuam ntawm koj kuajCov. Buffers pab tswj cov pH ruaj khov, uas yog qhov tseem ceeb rau kev tswj cov ionization xeev ntawm koj cov kws tshuaj ntsuam, zoo li lub sijhawm. Hauv qab no yog cov ncauj lus kom ntxaws saib ntawm yuav ua li cas xaiv qhov zoo tshaj plaws tsis rau koj cov ntawv HplC.

Koj puas paub txog HPLC qauv npaj? Nyem tsab xov xwm no los kawm ntxiv: "Txhua yam koj yuav tsum paub txog HPLC qauv npaj"

Qhov tseem ceeb ntawm Kev Xaiv Tsis

Ib tus ntas yog kev daws teeb meem uas nws lub v tsis hloov tom qab ntxiv cov kua qaub me me ntawm cov kua qaub lossis hauv paus. Raws li lawv cov muaj pes tsawg leeg, buffers tuaj yeem raug cais raws li cov kua qaub lossis theem pib. Qhov ua tau zoo ntawm kev ua tsis tau zoo yog txiav txim siab los ntawm nws PKA, uas yuav tsum muaj kev cuam tshuam nrog theem 1 Phem ntawm cov khoom lag luam txawb p rau kev ua kom zoo.

Qhov tseem ceeb los xav txog

1. Kab mob hauv ph ntawm lub sam thiaj yuav tsum muaj tsawg kawg 2 PHIB units deb ntawm Pka ntawm Analalte. Qhov no pab tau txhawm rau txhawm rau txhim kho ionization ntawm acidic lossis cov tshuaj ntsuam yooj yim, ua kom tiav lawv txoj kev tuav cia rau ntawm kem. Piv txwv li, yog tias tus kws tshuaj ntsuam yog cov kua qaub tsis muaj zog nrog PKA ntawm 4,5, siv cov theem mobile nrog PH nyob ib ncig ntawm 2.0 yuav khaws nws hauv lub xeev tsis yog-ionized, txhim kho lub xeev, txhim kho kev ua haujlwm.

2.Buffer Hom: nquag buffers siv hauv HPLC suav nrog:

Phosphate Buffers: Siv tau zoo nyob rau hauv lub Ph Thaj Tsam ntawm 2.0 rau 8.0, phosphate buffers tau siv dav vim lawv cov kev ruaj khov thiab kev sib raug zoo nrog ntau tus kws tshuaj. Lawv tsim tshwj xeeb rau LC-UV daim ntawv thov.

Acetate Buffers: Cov no tseem tuaj yeem nrov rau LC-UV xav tau thiab tuaj yeem siv rau cov phosphate zoo li cov txiv neej.

Volatile Buffers: rau cov ntawv thov lc, volatile buffers nyiam ua qauv, acetate, thiab ammonium bicarbonate yog lawv tuaj yeem muab tshem tawm yooj yim thaum lub cev cov txheej txheem loj.

3.buffer concentration: ib txwm pib qhov kev xav tau rau buffers nyob rau hauv HPLC yog nruab nrab ntawm 25-50 hli. Cov kev pab ntau dua tuaj yeem txhim kho cov duab siab zoo thiab ua tau zoo tab sis kuj tseem yuav ua rau muaj nag lossis daus thaum sib xyaw nrog cov kuab tshuaj organic. Nws raug nquahu kom ua kom lub tsis zoo hauv qab 25 hli kom tsis txhob muaj kev phom sij.

4. DiCemical purity: ib txwm siv high-puricaphic-buffers kom tiv thaiv kev sib kis thiab ua kom muaj txiaj ntsig zoo. Impurities tuaj yeem ua rau tsis muaj kev cuam tshuam thiab hloov siab sib txawv hauv kev khaws cia lub sijhawm.

Qhov cuam tshuam kub: Qhov ntsuas kub tuaj yeem cuam tshuam rau PH ntawm cov kev daws teeb meem. Nws yog ib qho tseem ceeb uas yuav tau npaj thiab khaws cov buffers ntawm qhov kub thiab txias kom tswj tau lawv cov hauj lwm zoo.

Xav paub 50 cov lus teb txog HPLC Vials, thov tshawb xyuas kab lus no: 50 Feem Ntau Cov Lus Nquag Nug ntawm HPLC Vials

Cov lus qhia ua tswv yim rau kev npaj ua ntas

Kev npaj kev qauv: Buffers tuaj yeem npaj los ntawm kev sib xyaw ua ke cov nyiaj ntawm lawv cov acidic thiab theem pib ntawm cov kua qaub. Zam txhob kho cov pH tom qab ntxiv cov kuab tshuaj organic, raws li qhov no tuaj yeem ua rau cov txiaj ntsig tau tshwm sim.

Cov kev daws teeb meem tshiab: Npaj cov kev daws teeb meem tshiab tsis tu ncua kom txo qis microbial kev loj hlob thiab qhov tsis txaus ntseeg ntawm cov khoom siv, uas tuaj yeem cuam tshuam rau cov kev ua tau zoo li cov ntxhiab.

Zam kev xeb: qee yam buffers, xws li crorate, tuaj yeem corrate stainless hlau Cheebtsam ntawm HPLNS. Xyuas kom meej meej flushing ntawm cov kab ke tom qab siv cov buffers los tiv thaiv kev puas tsuaj.

Tag

Xaiv txoj cai tsis rau koj HPLC Mobile theem yog qhov tseem ceeb rau kev ua tiav kev txhim khu kev qha thiab cov txiaj ntsig tau zoo. Txiav txim siab ph, hom kev ua yeeb yam, cov kev xav, thiab kev ua kom huv thaum xaiv. Los ntawm kev ua raws li cov lus qhia no, koj tuaj yeem ua kom zoo dua koj txoj kev HPLC kom muaj kev sib cais thiab txheeb xyuas koj cov qauv.

Xav paub tus nqi ntawm HPLC vials, thov tshawb xyuas kab lus no: HPLC Vials nqe: 50 Feem Ntau Cov Lus Nug

Yav dhau los:

Yuav ua li cas siv cov leeg ntsuas kom zoo rau cov dej ua haujlwm zoo?

Tom ntej:

Yuav ua li cas kom tsis txhob lim li ntawm cov kua lim